OCA at 16:56, 18 October 2023

OCA — Wed, 18 Oct 2023 16:56:32 GMT

←Older revision		Revision as of 16:56, 18 October 2023
Line 1:		Line 1:

-	====	+	==Structure of EmrE-D3 mutant in complex with monobody L10 and benzyltrimethylammonium==
-	<StructureSection load='7t00' size='340' side='right'caption='[[7t00]]' scene=''>	+	<StructureSection load='7t00' size='340' side='right'caption='[[7t00]], [[Resolution\|resolution]] 3.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7t00]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T00 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t00 OCA], [https://pdbe.org/7t00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t00 RCSB], [https://www.ebi.ac.uk/pdbsum/7t00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t00 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.91Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E00:benzyltrimethylammonium'>E00</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t00 OCA], [https://pdbe.org/7t00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t00 RCSB], [https://www.ebi.ac.uk/pdbsum/7t00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t00 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins from the bacterial small multidrug resistance (SMR) family are proton-coupled exporters of diverse antiseptics and antimicrobials, including polyaromatic cations and quaternary ammonium compounds. The transport mechanism of the Escherichia coli transporter, EmrE, has been studied extensively, but a lack of high-resolution structural information has impeded a structural description of its molecular mechanism. Here, we apply a novel approach, multipurpose crystallization chaperones, to solve several structures of EmrE, including a 2.9 A structure at low pH without substrate. We report five additional structures in complex with structurally diverse transported substrates, including quaternary phosphonium, quaternary ammonium, and planar polyaromatic compounds. These structures show that binding site tryptophan and glutamate residues adopt different rotamers to conform to disparate structures without requiring major rearrangements of the backbone structure. Structural and functional comparison to Gdx-Clo, an SMR protein that transports a much narrower spectrum of substrates, suggests that in EmrE, a relatively sparse hydrogen bond network among binding site residues permits increased sidechain flexibility.
		+
		+	Crystal structures of bacterial small multidrug resistance transporter EmrE in complex with structurally diverse substrates.,Kermani AA, Burata OE, Koff BB, Koide A, Koide S, Stockbridge RB Elife. 2022 Mar 7;11:e76766. doi: 10.7554/eLife.76766. PMID:35254261<ref>PMID:35254261</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7t00" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Kermani AA]]
		+	[[Category: Stockbridge RB]]

OCA at 06:22, 2 March 2022

OCA — Wed, 02 Mar 2022 06:22:11 GMT

←Older revision		Revision as of 06:22, 2 March 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7t00 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7t00' size='340' side='right'caption='[[7t00]]' scene=''>
-	~~Authors~~: ~~Kermani, A~~.A.~~, Stockbridge, R~~.B.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Structure of EmrE~~-~~D3 mutant in complex with monobody L10 and benzyltrimethylammonium~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t00 OCA], [https://pdbe.org/7t00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t00 RCSB], [https://www.ebi.ac.uk/pdbsum/7t00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t00 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Stockbridge, R.B~~]]	+	__TOC__
-	[[Category: ~~Kermani, A.A~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7t00" [edit=sysop:move=sysop]

OCA — Wed, 08 Dec 2021 08:36:17 GMT

Protected "7t00" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:36, 8 December 2021

OCA: New page: '''Unreleased structure''' The entry 7t00 is ON HOLD until Paper Publication Authors: Kermani, A.A., Stockbridge, R.B. Description: Structure of EmrE-D3 mutant in complex with monobody...

OCA — Wed, 08 Dec 2021 08:36:16 GMT

New page: '''Unreleased structure''' The entry 7t00 is ON HOLD until Paper Publication Authors: Kermani, A.A., Stockbridge, R.B. Description: Structure of EmrE-D3 mutant in complex with monobody...

New page

'''Unreleased structure'''

The entry 7t00 is ON HOLD until Paper Publication

Authors: Kermani, A.A., Stockbridge, R.B.

Description: Structure of EmrE-D3 mutant in complex with monobody L10 and benzyltrimethylammonium
[[Category: Unreleased Structures]]
[[Category: Stockbridge, R.B]]
[[Category: Kermani, A.A]]

7t00 - Revision history

OCA at 16:56, 18 October 2023

OCA at 06:22, 2 March 2022

OCA: Protected "7t00" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7t00 is ON HOLD until Paper Publication Authors: Kermani, A.A., Stockbridge, R.B. Description: Structure of EmrE-D3 mutant in complex with monobody...