7t03 - Revision history

OCA at 11:21, 14 June 2023

OCA — Wed, 14 Jun 2023 11:21:03 GMT

←Older revision		Revision as of 11:21, 14 June 2023
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	<StructureSection load='7t03' size='340' side='right'caption='[[7t03]]' scene=''>		<StructureSection load='7t03' size='340' side='right'caption='[[7t03]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T03 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7t03]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T03 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t03 OCA], [https://pdbe.org/7t03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t03 RCSB], [https://www.ebi.ac.uk/pdbsum/7t03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t03 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t03 OCA], [https://pdbe.org/7t03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t03 RCSB], [https://www.ebi.ac.uk/pdbsum/7t03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t03 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Understanding protein folding is crucial for protein sciences. The conformational spaces and energy landscapes of cold (unfolded) protein states, as well as the associated transitions, are hardly explored. Furthermore, it is not known how structure relates to the cooperativity of cold transitions, if cold and heat unfolded states are thermodynamically similar, and if cold states play important roles for protein function. We created the cold unfolding 4-helix bundle DCUB1 with a de novo designed bipartite hydrophilic/hydrophobic core featuring a hydrogen bond network which extends across the bundle in order to study the relative importance of hydrophobic versus hydrophilic protein-water interactions for cold unfolding. Structural and thermodynamic characterization resulted in the discovery of a complex energy landscape for cold transitions, while the heat unfolded state is a random coil. Below approximately 0 degrees C, the core of DCUB1 disintegrates in a largely cooperative manner, while a near-native helical content is retained. The resulting cold core-unfolded state is compact and features extensive internal dynamics. Below -5 degrees C, two additional cold transitions are seen, that is, (i) the formation of a water-mediated, compact, and highly dynamic dimer, and (ii) the onset of cold helix unfolding decoupled from cold core unfolding. Our results suggest that cold unfolding is initiated by the intrusion of water into the hydrophilic core network and that cooperativity can be tuned by varying the number of core hydrogen bond networks. Protein design has proven to be invaluable to explore the energy landscapes of cold states and to robustly test related theories.
		+
		+	From Protein Design to the Energy Landscape of a Cold Unfolding Protein.,Pulavarti SVSRK, Maguire JB, Yuen S, Harrison JS, Griffin J, Premkumar L, Esposito EA, Makhatadze GI, Garcia AE, Weiss TM, Snell EH, Kuhlman B, Szyperski T J Phys Chem B. 2022 Feb 17;126(6):1212-1231. doi: 10.1021/acs.jpcb.1c10750. Epub , 2022 Feb 7. PMID:35128921<ref>PMID:35128921</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7t03" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
	[[Category: Griffin J]]		[[Category: Griffin J]]
	[[Category: Kuhlman B]]		[[Category: Kuhlman B]]

OCA at 06:22, 2 March 2022

OCA — Wed, 02 Mar 2022 06:22:13 GMT

←Older revision		Revision as of 06:22, 2 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7t03 ~~is ON HOLD until Nov 29 2023~~	+	==NMR structure of a designed cold unfolding four helix bundle==
-		+	<StructureSection load='7t03' size='340' side='right'caption='[[7t03]]' scene=''>
-	~~Authors~~: ~~Pulavarti, S~~., ~~Szyperski, T~~., ~~Yuen, S~~., ~~Maguire, J~~., ~~Griffin, J~~., ~~Kuhlman, B~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T03 FirstGlance]. <br>
-	~~Description: NMR structure of a designed cold unfolding four helix bundle~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t03 OCA], [https://pdbe.org/7t03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t03 RCSB], [https://www.ebi.ac.uk/pdbsum/7t03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t03 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Pulavarti, S~~]]	+	__TOC__
-	[[Category: ~~Yuen, S~~]]	+	</StructureSection>
-	[[Category: ~~Griffin,~~ J]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kuhlman, B~~]]	+	[[Category: Griffin J]]
-	[[Category: Szyperski, T]]	+	[[Category: Kuhlman B]]
-	[[Category: ~~Maguire, J~~]]	+	[[Category: Maguire J]]
		+	[[Category: Pulavarti S]]
		+	[[Category: Szyperski T]]
		+	[[Category: Yuen S]]

OCA at 09:18, 5 January 2022

OCA — Wed, 05 Jan 2022 09:18:21 GMT

←Older revision		Revision as of 09:18, 5 January 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7t03 is ON HOLD	+	The entry 7t03 is ON HOLD until Nov 29 2023

	Authors: Pulavarti, S., Szyperski, T., Yuen, S., Maguire, J., Griffin, J., Kuhlman, B.		Authors: Pulavarti, S., Szyperski, T., Yuen, S., Maguire, J., Griffin, J., Kuhlman, B.

OCA: Protected "7t03" [edit=sysop:move=sysop]

OCA — Wed, 08 Dec 2021 08:36:27 GMT

Protected "7t03" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:36, 8 December 2021

OCA: New page: '''Unreleased structure''' The entry 7t03 is ON HOLD Authors: Pulavarti, S., Szyperski, T., Yuen, S., Maguire, J., Griffin, J., Kuhlman, B. Description: NMR structure of a designed col...

OCA — Wed, 08 Dec 2021 08:36:26 GMT

New page: '''Unreleased structure''' The entry 7t03 is ON HOLD Authors: Pulavarti, S., Szyperski, T., Yuen, S., Maguire, J., Griffin, J., Kuhlman, B. Description: NMR structure of a designed col...

New page

'''Unreleased structure'''

The entry 7t03 is ON HOLD

Authors: Pulavarti, S., Szyperski, T., Yuen, S., Maguire, J., Griffin, J., Kuhlman, B.

Description: NMR structure of a designed cold unfolding four helix bundle
[[Category: Unreleased Structures]]
[[Category: Pulavarti, S]]
[[Category: Yuen, S]]
[[Category: Griffin, J]]
[[Category: Kuhlman, B]]
[[Category: Szyperski, T]]
[[Category: Maguire, J]]