7t1n - Revision history

OCA at 06:54, 14 September 2022

OCA — Wed, 14 Sep 2022 06:54:45 GMT

←Older revision		Revision as of 06:54, 14 September 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7t1n is ~~ON HOLD until Paper~~ Publication	+	==Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif==
		+	<StructureSection load='7t1n' size='340' side='right'caption='[[7t1n]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7t1n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T1N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T1N FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t1n OCA], [https://pdbe.org/7t1n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t1n RCSB], [https://www.ebi.ac.uk/pdbsum/7t1n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t1n ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Productive transcriptional elongation of many cellular and viral mRNAs requires transcriptional factors to extract pTEFb from the 7SK snRNP by modulating the association between HEXIM and 7SK snRNA. In HIV-1, Tat binds to 7SK by displacing HEXIM. However, without the structure of the 7SK-HEXIM complex, the constraints that must be overcome for displacement remain unknown. Furthermore, while structure details of the Tat(NL4-3)-7SK complex have been elucidated, it is unclear how subtypes with more HEXIM-like Tat sequences accomplish displacement. Here we report the structures of HEXIM, Tat(G), and Tat(Fin) arginine rich motifs in complex with the apical stemloop-1 of 7SK. While most interactions between 7SK with HEXIM and Tat are similar, critical differences exist that guide function. First, the conformational plasticity of 7SK enables the formation of three different base pair configurations at a critical remodeling site, which allows for the modulation required for HEXIM binding and its subsequent displacement by Tat. Furthermore, the specific sequence variations observed in various Tat subtypes all converge on remodeling 7SK at this region. Second, we show that HEXIM primes its own displacement by causing specific local destabilization upon binding - a feature that is then exploited by Tat to bind 7SK more efficiently.

-	~~Authors: Pham, V.V~~., ~~Gao~~, M., D''Souza~~, V~~.M.	+	A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA.,Pham VV, Gao M, Meagher JL, Smith JL, D'Souza VM Commun Biol. 2022 Aug 15;5(1):819. doi: 10.1038/s42003-022-03734-w. PMID:35970937<ref>PMID:35970937</ref>

-	~~Description: Solution structure~~ of ~~7SK stem~~-~~loop 1 with HEXIM Arginine Rich Motif~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: D''Souza~~, V.M~~]]	+	<div class="pdbe-citations 7t1n" style="background-color:#fffaf0;"></div>
-	[[Category: Gao, M]]	+	== References ==
-	[[Category: Pham~~, V.V~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: D'Souza VM]]
		+	[[Category: Gao M]]
		+	[[Category: Pham VV]]

OCA at 06:37, 22 June 2022

OCA — Wed, 22 Jun 2022 06:37:55 GMT

←Older revision		Revision as of 06:37, 22 June 2022
Line 3:		Line 3:
	The entry 7t1n is ON HOLD until Paper Publication		The entry 7t1n is ON HOLD until Paper Publication

-	Authors: Pham, V.V., Gao, M., D'Souza, V.M.	+	Authors: Pham, V.V., Gao, M., D''Souza, V.M.

	Description: Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif		Description: Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: D'Souza, V.M]]	+	[[Category: D''Souza, V.M]]
	[[Category: Gao, M]]		[[Category: Gao, M]]
	[[Category: Pham, V.V]]		[[Category: Pham, V.V]]

OCA at 09:19, 5 January 2022

OCA — Wed, 05 Jan 2022 09:19:40 GMT

←Older revision		Revision as of 09:19, 5 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7t1n is ON HOLD	+	The entry 7t1n is ON HOLD until Paper Publication

	Authors: Pham, V.V., Gao, M., D'Souza, V.M.		Authors: Pham, V.V., Gao, M., D'Souza, V.M.
Line 7:		Line 7:
	Description: Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif		Description: Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: D'Souza, V.M]]
	[[Category: Gao, M]]		[[Category: Gao, M]]
	[[Category: Pham, V.V]]		[[Category: Pham, V.V]]
-	[[Category: D'Souza, V.M]]

OCA at 12:01, 29 December 2021

OCA — Wed, 29 Dec 2021 12:01:56 GMT

←Older revision		Revision as of 12:01, 29 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7t1n is ON HOLD ~~until sometime in the future~~	+	The entry 7t1n is ON HOLD

	Authors: Pham, V.V., Gao, M., D'Souza, V.M.		Authors: Pham, V.V., Gao, M., D'Souza, V.M.
Line 7:		Line 7:
	Description: Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif		Description: Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: D'Souza, V.M]]
	[[Category: Gao, M]]		[[Category: Gao, M]]
	[[Category: Pham, V.V]]		[[Category: Pham, V.V]]
		+	[[Category: D'Souza, V.M]]

OCA at 15:41, 15 December 2021

OCA — Wed, 15 Dec 2021 15:41:01 GMT

←Older revision		Revision as of 15:41, 15 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7t1n is ON HOLD	+	The entry 7t1n is ON HOLD until sometime in the future

	Authors: Pham, V.V., Gao, M., D'Souza, V.M.		Authors: Pham, V.V., Gao, M., D'Souza, V.M.

OCA: Protected "7t1n" [edit=sysop:move=sysop]

OCA — Wed, 08 Dec 2021 08:39:27 GMT

Protected "7t1n" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:39, 8 December 2021

OCA: New page: '''Unreleased structure''' The entry 7t1n is ON HOLD Authors: Pham, V.V., Gao, M., D'Souza, V.M. Description: Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif [[Cat...

OCA — Wed, 08 Dec 2021 08:39:26 GMT

New page: '''Unreleased structure''' The entry 7t1n is ON HOLD Authors: Pham, V.V., Gao, M., D'Souza, V.M. Description: Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif [[Cat...

New page

'''Unreleased structure'''

The entry 7t1n is ON HOLD

Authors: Pham, V.V., Gao, M., D'Souza, V.M.

Description: Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif
[[Category: Unreleased Structures]]
[[Category: D'Souza, V.M]]
[[Category: Gao, M]]
[[Category: Pham, V.V]]