OCA at 08:11, 7 December 2022

OCA — Wed, 07 Dec 2022 08:11:59 GMT

←Older revision		Revision as of 08:11, 7 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7t2g is ~~ON HOLD until Paper~~ Publication	+	==CryoEM structure of mu-opioid receptor - Gi protein complex bound to mitragynine pseudoindoxyl (MP)==
		+	<StructureSection load='7t2g' size='340' side='right'caption='[[7t2g]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7t2g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T2G FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=EIG:Mitragynine+pseudoindoxyl'>EIG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t2g OCA], [https://pdbe.org/7t2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t2g RCSB], [https://www.ebi.ac.uk/pdbsum/7t2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t2g ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GNAI1_HUMAN GNAI1_HUMAN] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:17635935</ref> <ref>PMID:17264214</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Drugs targeting the mu-opioid receptor (muOR) are the most effective analgesics available but are also associated with fatal respiratory depression through a pathway that remains unclear. Here we investigated the mechanistic basis of action of lofentanil (LFT) and mitragynine pseudoindoxyl (MP), two muOR agonists with different safety profiles. LFT, one of the most lethal opioids, and MP, a kratom plant derivative with reduced respiratory depression in animal studies, exhibited markedly different efficacy profiles for G protein subtype activation and beta-arrestin recruitment. Cryo-EM structures of muOR-Gi1 complex with MP (2.5 A) and LFT (3.2 A) revealed that the two ligands engage distinct subpockets, and molecular dynamics simulations showed additional differences in the binding site that promote distinct active-state conformations on the intracellular side of the receptor where G proteins and beta-arrestins bind. These observations highlight how drugs engaging different parts of the muOR orthosteric pocket can lead to distinct signaling outcomes.

-	~~Authors~~:	+	Insights into distinct signaling profiles of the microOR activated by diverse agonists.,Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G Nat Chem Biol. 2022 Nov 21. doi: 10.1038/s41589-022-01208-y. PMID:36411392<ref>PMID:36411392</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7t2g" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
		+	[[Category: Kobilka BK]]
		+	[[Category: Qu Q]]
		+	[[Category: Robertson MJ]]
		+	[[Category: Seven AB]]
		+	[[Category: Wang H]]

OCA at 08:34, 12 January 2022

OCA — Wed, 12 Jan 2022 08:34:47 GMT

←Older revision		Revision as of 08:34, 12 January 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7t2g is ON HOLD	+	The entry 7t2g is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7t2g" [edit=sysop:move=sysop]

OCA — Wed, 15 Dec 2021 15:42:01 GMT

Protected "7t2g" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:42, 15 December 2021

OCA: New page: '''Unreleased structure''' The entry 7t2g is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Dec 2021 15:41:59 GMT

New page: '''Unreleased structure''' The entry 7t2g is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7t2g is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7t2g - Revision history

OCA at 08:11, 7 December 2022

OCA at 08:34, 12 January 2022

OCA: Protected "7t2g" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7t2g is ON HOLD Authors: Description: Category: Unreleased Structures