7t3i - Revision history

OCA at 10:01, 15 March 2023

OCA — Wed, 15 Mar 2023 10:01:26 GMT

←Older revision		Revision as of 10:01, 15 March 2023
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-	====	+	==CryoEM structure of the Rix7 D2 Walker B mutant==
-	<StructureSection load='7t3i' size='340' side='right'caption='[[7t3i]]' scene=''>	+	<StructureSection load='7t3i' size='340' side='right'caption='[[7t3i]], [[Resolution\|resolution]] 4.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7t3i]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T3I FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t3i OCA], [https://pdbe.org/7t3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t3i RCSB], [https://www.ebi.ac.uk/pdbsum/7t3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t3i ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t3i OCA], [https://pdbe.org/7t3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t3i RCSB], [https://www.ebi.ac.uk/pdbsum/7t3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t3i ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G0RZG1_CHATD G0RZG1_CHATD]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rix7 is an essential AAA+ ATPase that functions during the early stages of ribosome biogenesis. Rix7 is composed of three domains including an N-terminal domain (NTD) and two AAA+ domains (D1 and D2) that assemble into an asymmetric stacked hexamer. It was recently established that Rix7 is a presumed protein translocase that removes substrates from preribosomes by translocating them through its central pore. However, how the different domains of Rix7 coordinate their activities within the overall hexameric structure was unknown. We captured cryo-electron microscopy (EM) structures of single and double Walker B variants of full length Rix7. The disordered NTD was not visible in the cryo-EM reconstructions, but cross-linking mass spectrometry revealed that the NTD can associate with the central channel in vitro. Deletion of the disordered NTD enabled us to obtain a structure of the Rix7 hexamer to 2.9 A resolution, providing high resolution details of critical motifs involved in substrate translocation and interdomain communication. This structure coupled with cell-based assays established that the linker connecting the D1 and D2 domains as well as the pore loops lining the central channel are essential for formation of the large ribosomal subunit. Together, our work shows that Rix7 utilizes a complex communication network to drive ribosome biogenesis.
		+
		+	Communication network within the essential AAA-ATPase Rix7 drives ribosome assembly.,Kocaman S, Lo YH, Krahn JM, Sobhany M, Dandey VP, Petrovich ML, Etigunta SK, Williams JG, Deterding LJ, Borgnia MJ, Stanley RE PNAS Nexus. 2022 Jul 21;1(4):pgac118. doi: 10.1093/pnasnexus/pgac118. eCollection , 2022 Sep. PMID:36090660<ref>PMID:36090660</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7t3i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chaetomium thermophilum]]
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Kocaman S]]
		+	[[Category: Stanley RE]]

OCA at 06:22, 2 March 2022

OCA — Wed, 02 Mar 2022 06:22:14 GMT

←Older revision		Revision as of 06:22, 2 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7t3i ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7t3i' size='340' side='right'caption='[[7t3i]]' scene=''>
-	~~Authors~~: ~~Kocaman, S~~.~~, Stanley, R~~.E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: CryoEM structure of~~ the ~~Rix7 D2 Walker B mutant~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t3i OCA], [https://pdbe.org/7t3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t3i RCSB], [https://www.ebi.ac.uk/pdbsum/7t3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t3i ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Kocaman, S~~]]	+	__TOC__
-	[[Category: ~~Stanley, R.E~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:35, 12 January 2022

OCA — Wed, 12 Jan 2022 08:35:30 GMT

←Older revision		Revision as of 08:35, 12 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7t3i is ON HOLD	+	The entry 7t3i is ON HOLD until Paper Publication

	Authors: Kocaman, S., Stanley, R.E.		Authors: Kocaman, S., Stanley, R.E.

OCA: Protected "7t3i" [edit=sysop:move=sysop]

OCA — Wed, 15 Dec 2021 15:44:10 GMT

Protected "7t3i" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:44, 15 December 2021

OCA: New page: '''Unreleased structure''' The entry 7t3i is ON HOLD Authors: Kocaman, S., Stanley, R.E. Description: CryoEM structure of the Rix7 D2 Walker B mutant [[Category: Unreleased Structures]...

OCA — Wed, 15 Dec 2021 15:44:09 GMT

New page: '''Unreleased structure''' The entry 7t3i is ON HOLD Authors: Kocaman, S., Stanley, R.E. Description: CryoEM structure of the Rix7 D2 Walker B mutant [[Category: Unreleased Structures]...

New page

'''Unreleased structure'''

The entry 7t3i is ON HOLD

Authors: Kocaman, S., Stanley, R.E.

Description: CryoEM structure of the Rix7 D2 Walker B mutant
[[Category: Unreleased Structures]]
[[Category: Kocaman, S]]
[[Category: Stanley, R.E]]