7t4l - Revision history

OCA at 08:46, 23 February 2022

OCA — Wed, 23 Feb 2022 08:46:05 GMT

←Older revision		Revision as of 08:46, 23 February 2022
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	==Structure of dimeric phosphorylated Pediculus humanus (Ph) PINK1 with extended alpha-C helix in chain B==		==Structure of dimeric phosphorylated Pediculus humanus (Ph) PINK1 with extended alpha-C helix in chain B==
-	<StructureSection load='7t4l' size='340' side='right'caption='[[7t4l]]' scene=''>	+	<StructureSection load='7t4l' size='340' side='right'caption='[[7t4l]], [[Resolution\|resolution]] 3.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T4L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7t4l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T4L FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t4l OCA], [https://pdbe.org/7t4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t4l RCSB], [https://www.ebi.ac.uk/pdbsum/7t4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t4l ProSAT]</span></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t4l OCA], [https://pdbe.org/7t4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t4l RCSB], [https://www.ebi.ac.uk/pdbsum/7t4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t4l ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mutations in the protein kinase PINK1 lead to defects in mitophagy and cause autosomal recessive early onset Parkinson's Disease (EOPD)(1,2). PINK1 has many unique features that enable it to phosphorylate ubiquitin and the ubiquitin-like domain of Parkin(3-9). Structural analysis of PINK1 from diverse insect species(10-12) with and without ubiquitin provided snapshots of distinct structural states yet did not explain how PINK1 is activated. We here elucidate the activation mechanism of PINK1 by crystallography and cryo-EM. A crystal structure of unphosphorylated Pediculus humanus corporis (Ph) PINK1 resolves a previously omitted N-terminal helix revealing how unphosphorylated yet active PINK1 is oriented on mitochondria. We further reveal a 2.35 A cryo-EM structure of a symmetric PhPINK1 dimer trapped during the process of trans-autophosphorylation, and a 3.1 A cryo-EM structure of phosphorylated PhPINK1 in the process of undergoing a conformational change to become an active ubiquitin kinase. Structures and phosphorylation studies further identify a role for regulatory PINK1 oxidation. Together, our work delineates the complete activation mechanism of PINK1, illuminates how PINK1 interacts with the mitochondrial outer membrane, and reveals how PINK1 activity may be modulated by mitochondrial reactive oxygen species.
		+
		+	Activation mechanism of PINK1.,Gan ZY, Callegari S, Cobbold SA, Cotton TR, Mlodzianoski MJ, Schubert AF, Geoghegan ND, Rogers KL, Leis A, Dewson G, Glukhova A, Komander D Nature. 2021 Dec 21. pii: 10.1038/s41586-021-04340-2. doi:, 10.1038/s41586-021-04340-2. PMID:34933320<ref>PMID:34933320</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7t4l" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dewson G]]	+	[[Category: Non-specific serine/threonine protein kinase]]
-	[[Category: Gan ZY]]	+	[[Category: Dewson, G]]
-	[[Category: Glukhova A]]	+	[[Category: Gan, Z Y]]
-	[[Category: Komander D]]	+	[[Category: Glukhova, A]]
-	[[Category: Leis A]]	+	[[Category: Komander, D]]
		+	[[Category: Leis, A]]
		+	[[Category: Kinase]]
		+	[[Category: Mitophagy]]
		+	[[Category: Parkinson's disease]]
		+	[[Category: Phospho-ubiquitin]]
		+	[[Category: Phosphorylation]]
		+	[[Category: Pink1]]
		+	[[Category: Transferase]]
		+	[[Category: Ubiquitin]]

OCA at 10:00, 12 January 2022

OCA — Wed, 12 Jan 2022 10:00:16 GMT

←Older revision		Revision as of 10:00, 12 January 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7t4l is ON HOLD~~	+	==Structure of dimeric phosphorylated Pediculus humanus (Ph) PINK1 with extended alpha-C helix in chain B==
-		+	<StructureSection load='7t4l' size='340' side='right'caption='[[7t4l]]' scene=''>
-	~~Authors: Gan, Z.Y., Leis, A., Dewson, G., Glukhova, A., Komander, D.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T4L FirstGlance]. <br>
-	~~Description:~~ Structure of dimeric phosphorylated Pediculus humanus (Ph) PINK1 with extended alpha-C helix in chain B	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t4l OCA], [https://pdbe.org/7t4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t4l RCSB], [https://www.ebi.ac.uk/pdbsum/7t4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t4l ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Dewson, G]]	+	__TOC__
-	[[Category: ~~Leis, A~~]]	+	</StructureSection>
-	[[Category: ~~Komander, D~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Glukhova, A~~]]	+	[[Category: Dewson G]]
-	[[Category: ~~Gan, Z.Y~~]]	+	[[Category: Gan ZY]]
		+	[[Category: Glukhova A]]
		+	[[Category: Komander D]]
		+	[[Category: Leis A]]

OCA: Protected "7t4l" [edit=sysop:move=sysop]

OCA — Wed, 15 Dec 2021 15:45:59 GMT

Protected "7t4l" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:45, 15 December 2021

OCA: New page: '''Unreleased structure''' The entry 7t4l is ON HOLD Authors: Gan, Z.Y., Leis, A., Dewson, G., Glukhova, A., Komander, D. Description: Structure of dimeric phosphorylated Pediculus hum...

OCA — Wed, 15 Dec 2021 15:45:58 GMT

New page: '''Unreleased structure''' The entry 7t4l is ON HOLD Authors: Gan, Z.Y., Leis, A., Dewson, G., Glukhova, A., Komander, D. Description: Structure of dimeric phosphorylated Pediculus hum...

New page

'''Unreleased structure'''

The entry 7t4l is ON HOLD

Authors: Gan, Z.Y., Leis, A., Dewson, G., Glukhova, A., Komander, D.

Description: Structure of dimeric phosphorylated Pediculus humanus (Ph) PINK1 with extended alpha-C helix in chain B
[[Category: Unreleased Structures]]
[[Category: Dewson, G]]
[[Category: Leis, A]]
[[Category: Komander, D]]
[[Category: Glukhova, A]]
[[Category: Gan, Z.Y]]