7t51 - Revision history

OCA at 17:00, 18 October 2023

OCA — Wed, 18 Oct 2023 17:00:07 GMT

←Older revision		Revision as of 17:00, 18 October 2023
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	<StructureSection load='7t51' size='340' side='right'caption='[[7t51]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='7t51' size='340' side='right'caption='[[7t51]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7t51]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T51 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7t51]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PA1 Pseudomonas aeruginosa PA1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T51 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height~~: ~~3em;~~'>~~[[7t4z\|7t4z]]~~, ~~[[7t50\|7t50]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t51 OCA], [https://pdbe.org/7t51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t51 RCSB], [https://www.ebi.ac.uk/pdbsum/7t51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t51 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t51 OCA], [https://pdbe.org/7t51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t51 RCSB], [https://www.ebi.ac.uk/pdbsum/7t51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t51 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MODA_PSEAE MODA_PSEAE] Part of the ABC transporter complex ModABC involved in the transport of molybdenum into the cell (Probable). Binds tungstate and molybdate (PubMed:31627455, PubMed:25172858). Can also bind chromate, with lower affinity (PubMed:31627455). Plays an essential role in recruitment of molybdate for nitrate reduction (PubMed:25172858).<ref>PMID:25172858</ref> <ref>PMID:31627455</ref> <ref>PMID:25172858</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Kobe, B~~]]	+	[[Category: Pseudomonas aeruginosa PA1]]
-	[[Category: ~~Lim,~~ B ~~Y.J~~]]	+	[[Category: Kobe B]]
-	[[Category: ~~Luo, Z~~]]	+	[[Category: Lim BYJ]]
-	[[Category: ~~Ngu, D H.Y~~]]	+	[[Category: Luo Z]]
-	[[Category: ~~Metal binding protein]]~~	+	[[Category: Ngu DHY]]
-	~~[[Category: Molybdate-binding periplasmic protein~~]]	+

OCA at 07:34, 29 June 2022

OCA — Wed, 29 Jun 2022 07:34:19 GMT

←Older revision		Revision as of 07:34, 29 June 2022
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	==Crystal structure of the molybdate-binding periplasmic protein ModA from the bacteria Pseudomonsa aeruginosa in molybdate-bound form==		==Crystal structure of the molybdate-binding periplasmic protein ModA from the bacteria Pseudomonsa aeruginosa in molybdate-bound form==
-	<StructureSection load='7t51' size='340' side='right'caption='[[7t51]]' scene=''>	+	<StructureSection load='7t51' size='340' side='right'caption='[[7t51]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T51 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7t51]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T51 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t51 OCA], [https://pdbe.org/7t51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t51 RCSB], [https://www.ebi.ac.uk/pdbsum/7t51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t51 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7t4z\|7t4z]], [[7t50\|7t50]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t51 OCA], [https://pdbe.org/7t51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t51 RCSB], [https://www.ebi.ac.uk/pdbsum/7t51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t51 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acquisition of the trace-element molybdenum via the high-affinity ATP-binding cassette permease ModABC is essential for Pseudomonas aeruginosa respiration in anaerobic and microaerophilic environments. This study determined the X-ray crystal structures of the molybdenum-recruiting solute-binding protein ModA from P. aeruginosa PAO1 in the metal-free state and bound to the group 6 metal oxyanions molybdate, tungstate, and chromate. Pseudomonas aeruginosa PAO1 ModA has a non-contiguous dual-hinged bilobal structure with a single metal-binding site positioned between the two domains. Metal binding results in a 22 degrees relative rotation of the two lobes with the oxyanions coordinated by four residues, that contribute six hydrogen bonds, distinct from ModA orthologues that feature an additional oxyanion-binding residue. Analysis of 485 Pseudomonas ModA sequences revealed conservation of the metal-binding residues and beta-sheet structural elements, highlighting their contribution to protein structure and function. Despite the capacity of ModA to bind chromate, deletion of modA did not affect P. aeruginosa PAO1 sensitivity to chromate toxicity nor impact cellular accumulation of chromate. Exposure to sub-inhibitory concentrations of chromate broadly perturbed P. aeruginosa metal homeostasis and, unexpectedly, was associated with an increase in ModA-mediated molybdenum uptake. Elemental analyses of the proteome from anaerobically grown P. aeruginosa revealed that, despite the increase in cellular molybdenum upon chromate exposure, distribution of the metal within the proteome was substantially perturbed. This suggested that molybdoprotein cofactor acquisition may be disrupted, consistent with the potent toxicity of chromate under anaerobic conditions. Collectively, these data reveal a complex relationship between chromate toxicity, molybdenum homeostasis and anaerobic respiration.
		+
		+	The Impact of Chromate on Pseudomonas aeruginosa Molybdenum Homeostasis.,Maunders EA, Ngu DHY, Ganio K, Hossain SI, Lim BYJ, Leeming MG, Luo Z, Tan A, Deplazes E, Kobe B, McDevitt CA Front Microbiol. 2022 May 24;13:903146. doi: 10.3389/fmicb.2022.903146., eCollection 2022. PMID:35685933<ref>PMID:35685933</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7t51" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kobe B]]	+	[[Category: Kobe, B]]
-	[[Category: Lim ~~BYJ~~]]	+	[[Category: Lim, B Y.J]]
-	[[Category: Luo Z]]	+	[[Category: Luo, Z]]
-	[[Category: Ngu ~~DHY~~]]	+	[[Category: Ngu, D H.Y]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Molybdate-binding periplasmic protein]]

OCA at 12:50, 4 May 2022

OCA — Wed, 04 May 2022 12:50:44 GMT

←Older revision		Revision as of 12:50, 4 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7t51 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the molybdate-binding periplasmic protein ModA from the bacteria Pseudomonsa aeruginosa in molybdate-bound form==
-		+	<StructureSection load='7t51' size='340' side='right'caption='[[7t51]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T51 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t51 OCA], [https://pdbe.org/7t51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t51 RCSB], [https://www.ebi.ac.uk/pdbsum/7t51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t51 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kobe B]]
		+	[[Category: Lim BYJ]]
		+	[[Category: Luo Z]]
		+	[[Category: Ngu DHY]]

OCA at 12:02, 29 December 2021

OCA — Wed, 29 Dec 2021 12:02:28 GMT

←Older revision		Revision as of 12:02, 29 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7t51 is ON HOLD	+	The entry 7t51 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7t51" [edit=sysop:move=sysop]

OCA — Wed, 22 Dec 2021 14:04:25 GMT

Protected "7t51" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:04, 22 December 2021

OCA: New page: '''Unreleased structure''' The entry 7t51 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Dec 2021 14:04:24 GMT

New page: '''Unreleased structure''' The entry 7t51 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7t51 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]