7t81 - Revision history

OCA at 13:07, 1 March 2024

OCA — Fri, 01 Mar 2024 13:07:37 GMT

←Older revision		Revision as of 13:07, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='7t81' size='340' side='right'caption='[[7t81]], [[Resolution\|resolution]] 10.00Å' scene=''>		<StructureSection load='7t81' size='340' side='right'caption='[[7t81]], [[Resolution\|resolution]] 10.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7t81]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T81 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7t81]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T81 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[7t7c\|7t7c]]~~, [[~~7t7r~~\|~~7t7r~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 10Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t81 OCA], [https://pdbe.org/7t81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t81 RCSB], [https://www.ebi.ac.uk/pdbsum/7t81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t81 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t81 OCA], [https://pdbe.org/7t81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t81 RCSB], [https://www.ebi.ac.uk/pdbsum/7t81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t81 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/UN13A_RAT UN13A_RAT]] Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Also involved in secretory granule priming in insulin secretion.<ref>PMID:9697857</ref> <ref>PMID:11343654</ref> <ref>PMID:11792326</ref>	+	[https://www.uniprot.org/uniprot/UN13A_RAT UN13A_RAT] Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Also involved in secretory granule priming in insulin secretion.<ref>PMID:9697857</ref> <ref>PMID:11343654</ref> <ref>PMID:11792326</ref> [https://www.uniprot.org/uniprot/UN13A_MOUSE UN13A_MOUSE] Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Facilitates neuronal dense core vesicles fusion as well as controls the location and efficiency of their synaptic release (PubMed:23229896). Also involved in secretory granule priming in insulin secretion. Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB:Q62768][UniProtKB:Q9UPW8]<ref>PMID:10440375</ref> <ref>PMID:12070347</ref> <ref>PMID:15988013</ref> <ref>PMID:16644700</ref> <ref>PMID:16697276</ref> <ref>PMID:17267576</ref> <ref>PMID:17639022</ref> <ref>PMID:23229896</ref>
-	~~<div style="background-color~~:~~#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~How can exactly six SNARE complexes be assembled under each synaptic~~ vesicle~~? Here we report cryo-EM crystal structures~~ of the ~~core domain of Munc13, the key chaperone that initiates SNAREpin assembly~~. ~~The functional core of Munc13, consisting of C1-C2B-MUN-C2C (Munc13C) spontaneously crystallizes between phosphatidylserine-rich bilayers~~ in ~~two distinct conformations, each in a radically different oligomeric state. In the open conformation (state 1), Munc13C forms upright trimers that link the two bilayers, separating them~~ by ~~approximately 21 nm. In the closed conformation, six copies of Munc13C interact~~ to ~~form a lateral hexamer elevated approximately 14 nm above the bilayer. Open~~ and ~~closed conformations differ only by a rigid body rotation around a flexible hinge, which when performed cooperatively assembles Munc13 into a lateral hexamer (state 2)~~ in ~~which~~ the ~~key SNARE assembly~~-~~activating site~~ of ~~Munc13 is autoinhibited by its neighbor~~. We propose that each Munc13 in the lateral hexamer ultimately assembles a single SNAREpin, explaining how only and exactly six SNARE complexes are templated. We suggest that state 1 and state 2 may represent two successive states in the synaptic vesicle ~~supply chain leading to "primed" ready~~-~~release~~ vesicles ~~in which SNAREpins are clamped~~ and ~~ready to~~ release (~~state 3~~).	+
-		+
-	~~Munc13 structural transitions and oligomers that may choreograph successive stages~~ in ~~vesicle~~ priming ~~for neurotransmitter release~~.~~,Grushin K, Kalyana Sundaram RV, Sindelar CV, Rothman JE Proc Natl Acad Sci U S A. 2022 Feb 15;119~~(7). ~~pii~~: ~~2121259119. doi~~:~~, 10.1073~~/~~pnas.2121259119.~~ PMID:~~35135883~~<ref>PMID:~~35135883~~</ref>	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 7t81" style="background-color~~:~~#fffaf0;"~~></~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Grushin, K~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: ~~Sindelar, C V~~]]	+	[[Category: Grushin K]]
-	[[Category: ~~Exocytosis]]~~	+	[[Category: Sindelar CV]]
-	~~[[Category: Membrane fusion]]~~	+
-	~~[[Category: Munc13]]~~	+
-	~~[[Category: Synaptic transmission~~]]	+

OCA at 08:47, 23 February 2022

OCA — Wed, 23 Feb 2022 08:47:36 GMT

←Older revision		Revision as of 08:47, 23 February 2022
Line 1:		Line 1:

	==Model of Munc13-1 C1-C2B-MUN-C2C 2D crystal between lipid bilayers.==		==Model of Munc13-1 C1-C2B-MUN-C2C 2D crystal between lipid bilayers.==
-	<StructureSection load='7t81' size='340' side='right'caption='[[7t81]]' scene=''>	+	<StructureSection load='7t81' size='340' side='right'caption='[[7t81]], [[Resolution\|resolution]] 10.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T81 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7t81]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T81 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t81 OCA], [https://pdbe.org/7t81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t81 RCSB], [https://www.ebi.ac.uk/pdbsum/7t81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t81 ProSAT]</span></td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7t7c\|7t7c]], [[7t7r\|7t7r]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t81 OCA], [https://pdbe.org/7t81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t81 RCSB], [https://www.ebi.ac.uk/pdbsum/7t81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t81 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/UN13A_RAT UN13A_RAT]] Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Also involved in secretory granule priming in insulin secretion.<ref>PMID:9697857</ref> <ref>PMID:11343654</ref> <ref>PMID:11792326</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	How can exactly six SNARE complexes be assembled under each synaptic vesicle? Here we report cryo-EM crystal structures of the core domain of Munc13, the key chaperone that initiates SNAREpin assembly. The functional core of Munc13, consisting of C1-C2B-MUN-C2C (Munc13C) spontaneously crystallizes between phosphatidylserine-rich bilayers in two distinct conformations, each in a radically different oligomeric state. In the open conformation (state 1), Munc13C forms upright trimers that link the two bilayers, separating them by approximately 21 nm. In the closed conformation, six copies of Munc13C interact to form a lateral hexamer elevated approximately 14 nm above the bilayer. Open and closed conformations differ only by a rigid body rotation around a flexible hinge, which when performed cooperatively assembles Munc13 into a lateral hexamer (state 2) in which the key SNARE assembly-activating site of Munc13 is autoinhibited by its neighbor. We propose that each Munc13 in the lateral hexamer ultimately assembles a single SNAREpin, explaining how only and exactly six SNARE complexes are templated. We suggest that state 1 and state 2 may represent two successive states in the synaptic vesicle supply chain leading to "primed" ready-release vesicles in which SNAREpins are clamped and ready to release (state 3).
		+
		+	Munc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release.,Grushin K, Kalyana Sundaram RV, Sindelar CV, Rothman JE Proc Natl Acad Sci U S A. 2022 Feb 15;119(7). pii: 2121259119. doi:, 10.1073/pnas.2121259119. PMID:35135883<ref>PMID:35135883</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7t81" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Grushin K]]	+	[[Category: Grushin, K]]
-	[[Category: Sindelar CV]]	+	[[Category: Sindelar, C V]]
		+	[[Category: Exocytosis]]
		+	[[Category: Membrane fusion]]
		+	[[Category: Munc13]]
		+	[[Category: Synaptic transmission]]

OCA at 07:26, 9 February 2022

OCA — Wed, 09 Feb 2022 07:26:27 GMT

←Older revision		Revision as of 07:26, 9 February 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7t81 is ON HOLD~~	+	==Model of Munc13-1 C1-C2B-MUN-C2C 2D crystal between lipid bilayers.==
-		+	<StructureSection load='7t81' size='340' side='right'caption='[[7t81]]' scene=''>
-	~~Authors: Grushin, K., Sindelar, C.V.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T81 FirstGlance]. <br>
-	~~Description:~~ Model of Munc13-1 C1-C2B-MUN-C2C 2D crystal between lipid bilayers.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t81 OCA], [https://pdbe.org/7t81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t81 RCSB], [https://www.ebi.ac.uk/pdbsum/7t81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t81 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Sindelar~~, C.V]]	+	__TOC__
-	[[Category: Grushin, K]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Grushin K]]
		+	[[Category: Sindelar CV]]

OCA: Protected "7t81" [edit=sysop:move=sysop]

OCA — Wed, 22 Dec 2021 14:09:49 GMT

Protected "7t81" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:09, 22 December 2021

OCA: New page: '''Unreleased structure''' The entry 7t81 is ON HOLD Authors: Grushin, K., Sindelar, C.V. Description: Model of Munc13-1 C1-C2B-MUN-C2C 2D crystal between lipid bilayers. [[Category: U...

OCA — Wed, 22 Dec 2021 14:09:47 GMT

New page: '''Unreleased structure''' The entry 7t81 is ON HOLD Authors: Grushin, K., Sindelar, C.V. Description: Model of Munc13-1 C1-C2B-MUN-C2C 2D crystal between lipid bilayers. [[Category: U...

New page

'''Unreleased structure'''

The entry 7t81 is ON HOLD

Authors: Grushin, K., Sindelar, C.V.

Description: Model of Munc13-1 C1-C2B-MUN-C2C 2D crystal between lipid bilayers.
[[Category: Unreleased Structures]]
[[Category: Sindelar, C.V]]
[[Category: Grushin, K]]