OCA at 10:32, 2 August 2023

OCA — Wed, 02 Aug 2023 10:32:29 GMT

←Older revision		Revision as of 10:32, 2 August 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7tcn is ON HOLD~~	+	==Cryo-EM structure of CH235.12 in complex with HIV-1 Env trimer CH505TF.N279K.SOSIP.664 with high-mannose glycans==
-		+	<StructureSection load='7tcn' size='340' side='right'caption='[[7tcn]], [[Resolution\|resolution]] 4.10Å' scene=''>
-	~~Authors: Manne, K., Acharya, P.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[7tcn]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TCN FirstGlance]. <br>
-	~~Description:~~ Cryo-EM structure of CH235.12 in complex with HIV-1 Env trimer CH505TF.N279K.SOSIP.664 with high-mannose glycans	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.1Å</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[Category: Acharya, P]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tcn OCA], [https://pdbe.org/7tcn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tcn RCSB], [https://www.ebi.ac.uk/pdbsum/7tcn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tcn ProSAT]</span></td></tr>
-	[[Category: Manne, K]]	+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/M4M0W3_9HIV1 M4M0W3_9HIV1] Envelope glycoprotein gp160: Oligomerizes in the host endoplasmic reticulum into predominantly trimers. In a second time, gp160 transits in the host Golgi, where glycosylation is completed. The precursor is then proteolytically cleaved in the trans-Golgi and thereby activated by cellular furin or furin-like proteases to produce gp120 and gp41.[HAMAP-Rule:MF_04083] Surface protein gp120: Attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. This interaction induces a structural rearrangement creating a high affinity binding site for a chemokine coreceptor like CXCR4 and/or CCR5. Acts as a ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses. These interactions allow capture of viral particles at mucosal surfaces by these cells and subsequent transmission to permissive cells. HIV subverts the migration properties of dendritic cells to gain access to CD4+ T-cells in lymph nodes. Virus transmission to permissive T-cells occurs either in trans (without DCs infection, through viral capture and transmission), or in cis (following DCs productive infection, through the usual CD4-gp120 interaction), thereby inducing a robust infection. In trans infection, bound virions remain infectious over days and it is proposed that they are not degraded, but protected in non-lysosomal acidic organelles within the DCs close to the cell membrane thus contributing to the viral infectious potential during DCs' migration from the periphery to the lymphoid tissues. On arrival at lymphoid tissues, intact virions recycle back to DCs' cell surface allowing virus transmission to CD4+ T-cells.[HAMAP-Rule:MF_04083] Transmembrane protein gp41: Acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane. The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes. Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.[HAMAP-Rule:MF_04083]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Human immunodeficiency virus 1]]
		+	[[Category: Large Structures]]
		+	[[Category: Acharya P]]
		+	[[Category: Henderson R]]
		+	[[Category: Manne K]]

OCA at 03:49, 8 June 2022

OCA — Wed, 08 Jun 2022 03:49:32 GMT

←Older revision		Revision as of 03:49, 8 June 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7tcn is ON HOLD ~~until Paper Publication~~	+	The entry 7tcn is ON HOLD

	Authors: Manne, K., Acharya, P.		Authors: Manne, K., Acharya, P.

OCA at 06:16, 9 February 2022

OCA — Wed, 09 Feb 2022 06:16:15 GMT

←Older revision		Revision as of 06:16, 9 February 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7tcn is ON HOLD	+	The entry 7tcn is ON HOLD until Paper Publication

	Authors: Manne, K., Acharya, P.		Authors: Manne, K., Acharya, P.

OCA: Protected "7tcn" [edit=sysop:move=sysop]

OCA — Wed, 05 Jan 2022 09:20:59 GMT

Protected "7tcn" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:20, 5 January 2022

OCA: New page: '''Unreleased structure''' The entry 7tcn is ON HOLD Authors: Manne, K., Acharya, P. Description: Cryo-EM structure of CH235.12 in complex with HIV-1 Env trimer CH505TF.N279K.SOSIP.664...

OCA — Wed, 05 Jan 2022 09:20:58 GMT

New page: '''Unreleased structure''' The entry 7tcn is ON HOLD Authors: Manne, K., Acharya, P. Description: Cryo-EM structure of CH235.12 in complex with HIV-1 Env trimer CH505TF.N279K.SOSIP.664...

New page

'''Unreleased structure'''

The entry 7tcn is ON HOLD

Authors: Manne, K., Acharya, P.

Description: Cryo-EM structure of CH235.12 in complex with HIV-1 Env trimer CH505TF.N279K.SOSIP.664 with high-mannose glycans
[[Category: Unreleased Structures]]
[[Category: Acharya, P]]
[[Category: Manne, K]]

7tcn - Revision history

OCA at 10:32, 2 August 2023

OCA at 03:49, 8 June 2022

OCA at 06:16, 9 February 2022

OCA: Protected "7tcn" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7tcn is ON HOLD Authors: Manne, K., Acharya, P. Description: Cryo-EM structure of CH235.12 in complex with HIV-1 Env trimer CH505TF.N279K.SOSIP.664...