7tfi - Revision history

OCA at 20:05, 16 November 2022

OCA — Wed, 16 Nov 2022 20:05:02 GMT

←Older revision		Revision as of 20:05, 16 November 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7tfi is ~~ON HOLD until Paper~~ Publication	+	==Atomic model of the S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to DNA with an open clamp==
		+	<StructureSection load='7tfi' size='340' side='right'caption='[[7tfi]], [[Resolution\|resolution]] 3.41Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7tfi]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TFI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tfi OCA], [https://pdbe.org/7tfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tfi RCSB], [https://www.ebi.ac.uk/pdbsum/7tfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tfi ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RFC1_YEAST RFC1_YEAST] Component of the ATP-dependent clamp loader RFC complex for the POL30/PCNA homotrimer DNA clamp. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Replication factor C (RFC or activator 1) complex acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RFC uses ATP to assemble PCNA onto primed sites for replicative DNA polymerases delta and epsilon. The RFC pentamer forms a central chamber that binds 3' ss/ds DNA junctions to load PCNA onto DNA during replication. We show here five structures that identify a second DNA binding site in RFC that binds a 5' duplex. This 5' DNA site is located between the N-terminal BRCT domain and AAA+ module of the large Rfc1 subunit. Our structures reveal ideal binding to a 7-nt gap, which includes 2 bp unwound by the clamp loader. Biochemical studies show enhanced binding to 5 and 10 nt gaps, consistent with the structural results. Because both 3' and 5' ends are present at a ssDNA gap, we propose that the 5' site facilitates RFC's PCNA loading activity at a DNA damage-induced gap to recruit gap-filling polymerases. These findings are consistent with genetic studies showing that base excision repair of gaps greater than 1 base requires PCNA and involves the 5' DNA binding domain of Rfc1. We further observe that a 5' end facilitates PCNA loading at an RPA coated 30-nt gap, suggesting a potential role of the RFC 5'-DNA site in lagging strand DNA synthesis.

-	~~Authors: Zheng~~, F., Georgescu, R., Yao, ~~Y.N.~~, O''Donnell~~, M~~.E.~~, Li, H~~.	+	Cryo-EM structures reveal that RFC recognizes both the 3'- and 5'-DNA ends to load PCNA onto gaps for DNA repair.,Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME Elife. 2022 Jul 13;11. pii: 77469. doi: 10.7554/eLife.77469. PMID:35829698<ref>PMID:35829698</ref>

-	~~Description: Atomic model~~ of the S. ~~cerevisiae clamp~~-~~clamp loader complex PCNA~~-~~RFC bound to DNA with an open clamp~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Li, H~~]]	+	<div class="pdbe-citations 7tfi" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Zheng, F~~]]	+	== References ==
-	[[Category: O''Donnell~~, M.E~~]]	+	<references/>
-	[[Category: ~~Georgescu, R~~]]	+	__TOC__
-	[[Category: ~~Yao, Y.N~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Georgescu R]]
		+	[[Category: Li H]]
		+	[[Category: O'Donnell ME]]
		+	[[Category: Yao YN]]
		+	[[Category: Zheng F]]

OCA at 06:38, 22 June 2022

OCA — Wed, 22 Jun 2022 06:38:51 GMT

←Older revision		Revision as of 06:38, 22 June 2022
Line 3:		Line 3:
	The entry 7tfi is ON HOLD until Paper Publication		The entry 7tfi is ON HOLD until Paper Publication

-	Authors: Zheng, F., Georgescu, R., Yao, Y.N., O'Donnell, M.E., Li, H.	+	Authors: Zheng, F., Georgescu, R., Yao, Y.N., O''Donnell, M.E., Li, H.

	Description: Atomic model of the S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to DNA with an open clamp		Description: Atomic model of the S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to DNA with an open clamp
Line 9:		Line 9:
	[[Category: Li, H]]		[[Category: Li, H]]
	[[Category: Zheng, F]]		[[Category: Zheng, F]]
		+	[[Category: O''Donnell, M.E]]
	[[Category: Georgescu, R]]		[[Category: Georgescu, R]]
-	[[Category: O'Donnell, M.E]]
	[[Category: Yao, Y.N]]		[[Category: Yao, Y.N]]

OCA at 05:59, 19 January 2022

OCA — Wed, 19 Jan 2022 05:59:11 GMT

←Older revision		Revision as of 05:59, 19 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7tfi is ON HOLD	+	The entry 7tfi is ON HOLD until Paper Publication

	Authors: Zheng, F., Georgescu, R., Yao, Y.N., O'Donnell, M.E., Li, H.		Authors: Zheng, F., Georgescu, R., Yao, Y.N., O'Donnell, M.E., Li, H.
Line 7:		Line 7:
	Description: Atomic model of the S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to DNA with an open clamp		Description: Atomic model of the S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to DNA with an open clamp
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Georgescu, R]]
	[[Category: Li, H]]		[[Category: Li, H]]
	[[Category: Zheng, F]]		[[Category: Zheng, F]]
		+	[[Category: Georgescu, R]]
	[[Category: O'Donnell, M.E]]		[[Category: O'Donnell, M.E]]
	[[Category: Yao, Y.N]]		[[Category: Yao, Y.N]]

OCA: Protected "7tfi" [edit=sysop:move=sysop]

OCA — Wed, 12 Jan 2022 08:40:41 GMT

Protected "7tfi" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:40, 12 January 2022

OCA: New page: '''Unreleased structure''' The entry 7tfi is ON HOLD Authors: Zheng, F., Georgescu, R., Yao, Y.N., O'Donnell, M.E., Li, H. Description: Atomic model of the S. cerevisiae clamp-clamp lo...

OCA — Wed, 12 Jan 2022 08:40:40 GMT

New page: '''Unreleased structure''' The entry 7tfi is ON HOLD Authors: Zheng, F., Georgescu, R., Yao, Y.N., O'Donnell, M.E., Li, H. Description: Atomic model of the S. cerevisiae clamp-clamp lo...

New page

'''Unreleased structure'''

The entry 7tfi is ON HOLD

Authors: Zheng, F., Georgescu, R., Yao, Y.N., O'Donnell, M.E., Li, H.

Description: Atomic model of the S. cerevisiae clamp-clamp loader complex PCNA-RFC bound to DNA with an open clamp
[[Category: Unreleased Structures]]
[[Category: Georgescu, R]]
[[Category: Li, H]]
[[Category: Zheng, F]]
[[Category: O'Donnell, M.E]]
[[Category: Yao, Y.N]]