7tfs - Revision history

OCA at 20:24, 16 November 2022

OCA — Wed, 16 Nov 2022 20:24:47 GMT

←Older revision		Revision as of 20:24, 16 November 2022
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	==Cryo-EM of the OmcE nanowires from Geobacter sulfurreducens==		==Cryo-EM of the OmcE nanowires from Geobacter sulfurreducens==
-	<StructureSection load='7tfs' size='340' side='right'caption='[[7tfs]]' scene=''>	+	<StructureSection load='7tfs' size='340' side='right'caption='[[7tfs]], [[Resolution\|resolution]] 4.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TFS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7tfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TFS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tfs OCA], [https://pdbe.org/7tfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tfs RCSB], [https://www.ebi.ac.uk/pdbsum/7tfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tfs ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tfs OCA], [https://pdbe.org/7tfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tfs RCSB], [https://www.ebi.ac.uk/pdbsum/7tfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tfs ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q74FJ0_GEOSL Q74FJ0_GEOSL]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens were first observed two decades ago, with genetic and biochemical data suggesting that conductive fibres were type IV pili. Recently, an extracellular conductive filament of G. sulfurreducens was found to contain polymerized c-type cytochrome OmcS subunits, not pilin subunits. Here we report that G. sulfurreducens also produces a second, thinner appendage comprised of cytochrome OmcE subunits and solve its structure using cryo-electron microscopy at ~4.3 A resolution. Although OmcE and OmcS subunits have no overall sequence or structural similarities, upon polymerization both form filaments that share a conserved haem packing arrangement in which haems are coordinated by histidines in adjacent subunits. Unlike OmcS filaments, OmcE filaments are highly glycosylated. In extracellular fractions from G. sulfurreducens, we detected type IV pili comprising PilA-N and -C chains, along with abundant B-DNA. OmcE is the second cytochrome filament to be characterized using structural and biophysical methods. We propose that there is a broad class of conductive bacterial appendages with conserved haem packing (rather than sequence homology) that enable long-distance electron transport to chemicals or other microbial cells.
		+
		+	Cryo-EM structure of an extracellular Geobacter OmcE cytochrome filament reveals tetrahaem packing.,Wang F, Mustafa K, Suciu V, Joshi K, Chan CH, Choi S, Su Z, Si D, Hochbaum AI, Egelman EH, Bond DR Nat Microbiol. 2022 Aug;7(8):1291-1300. doi: 10.1038/s41564-022-01159-z. Epub, 2022 Jul 7. PMID:35798889<ref>PMID:35798889</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7tfs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Geobacter sulfurreducens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bond DR]]		[[Category: Bond DR]]

OCA at 12:51, 4 May 2022

OCA — Wed, 04 May 2022 12:51:39 GMT

←Older revision		Revision as of 12:51, 4 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7tfs ~~is ON HOLD until Paper Publication~~	+	==Cryo-EM of the OmcE nanowires from Geobacter sulfurreducens==
-		+	<StructureSection load='7tfs' size='340' side='right'caption='[[7tfs]]' scene=''>
-	~~Authors~~: ~~Wang, F~~.~~, Mustafa, K~~., ~~Chan, C~~.H., ~~Joshi, K~~., ~~Hochbaum, A~~.I., ~~Bond, D~~.R., ~~Egelman, E~~.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TFS FirstGlance]. <br>
-	~~Description: Cryo-EM of the OmcE nanowires from Geobacter sulfurreducens~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tfs OCA], [https://pdbe.org/7tfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tfs RCSB], [https://www.ebi.ac.uk/pdbsum/7tfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tfs ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Hochbaum, A.I~~]]	+	__TOC__
-	[[Category: ~~Egelman, E.H~~]]	+	</StructureSection>
-	[[Category: ~~Wang, F~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Bond, D.R~~]]	+	[[Category: Bond DR]]
-	[[Category: ~~Chan, C.H~~]]	+	[[Category: Chan CH]]
-	[[Category: ~~Joshi,~~ K]]	+	[[Category: Egelman EH]]
-	[[Category: ~~Mustafa, K~~]]	+	[[Category: Hochbaum AI]]
		+	[[Category: Joshi K]]
		+	[[Category: Mustafa K]]
		+	[[Category: Wang F]]

OCA at 09:06, 16 February 2022

OCA — Wed, 16 Feb 2022 09:06:37 GMT

←Older revision		Revision as of 09:06, 16 February 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7tfs is ON HOLD	+	The entry 7tfs is ON HOLD until Paper Publication

	Authors: Wang, F., Mustafa, K., Chan, C.H., Joshi, K., Hochbaum, A.I., Bond, D.R., Egelman, E.H.		Authors: Wang, F., Mustafa, K., Chan, C.H., Joshi, K., Hochbaum, A.I., Bond, D.R., Egelman, E.H.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Hochbaum, A.I]]		[[Category: Hochbaum, A.I]]
-	[[Category: Bond, D.R]]
-	[[Category: Chan, C.H]]
-	[[Category: Mustafa, K]]
	[[Category: Egelman, E.H]]		[[Category: Egelman, E.H]]
	[[Category: Wang, F]]		[[Category: Wang, F]]
		+	[[Category: Bond, D.R]]
		+	[[Category: Chan, C.H]]
	[[Category: Joshi, K]]		[[Category: Joshi, K]]
		+	[[Category: Mustafa, K]]

OCA: Protected "7tfs" [edit=sysop:move=sysop]

OCA — Wed, 19 Jan 2022 05:59:23 GMT

Protected "7tfs" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:59, 19 January 2022

OCA: New page: '''Unreleased structure''' The entry 7tfs is ON HOLD Authors: Wang, F., Mustafa, K., Chan, C.H., Joshi, K., Hochbaum, A.I., Bond, D.R., Egelman, E.H. Description: Cryo-EM of the OmcE n...

OCA — Wed, 19 Jan 2022 05:59:21 GMT

New page: '''Unreleased structure''' The entry 7tfs is ON HOLD Authors: Wang, F., Mustafa, K., Chan, C.H., Joshi, K., Hochbaum, A.I., Bond, D.R., Egelman, E.H. Description: Cryo-EM of the OmcE n...

New page

'''Unreleased structure'''

The entry 7tfs is ON HOLD

Authors: Wang, F., Mustafa, K., Chan, C.H., Joshi, K., Hochbaum, A.I., Bond, D.R., Egelman, E.H.

Description: Cryo-EM of the OmcE nanowires from Geobacter sulfurreducens
[[Category: Unreleased Structures]]
[[Category: Hochbaum, A.I]]
[[Category: Bond, D.R]]
[[Category: Chan, C.H]]
[[Category: Mustafa, K]]
[[Category: Egelman, E.H]]
[[Category: Wang, F]]
[[Category: Joshi, K]]