7toi - Revision history

OCA at 07:34, 3 November 2022

OCA — Thu, 03 Nov 2022 07:34:10 GMT

←Older revision		Revision as of 07:34, 3 November 2022
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	==Crystal structure of carbohydrate esterase PbeAcXE, in complex with acetate==		==Crystal structure of carbohydrate esterase PbeAcXE, in complex with acetate==
-	<StructureSection load='7toi' size='340' side='right'caption='[[7toi]]' scene=''>	+	<StructureSection load='7toi' size='340' side='right'caption='[[7toi]], [[Resolution\|resolution]] 1.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TOI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7toi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Prolixibacter_bellariivorans Prolixibacter bellariivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TOI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7toi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7toi OCA], [https://pdbe.org/7toi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7toi RCSB], [https://www.ebi.ac.uk/pdbsum/7toi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7toi ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7toi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7toi OCA], [https://pdbe.org/7toi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7toi RCSB], [https://www.ebi.ac.uk/pdbsum/7toi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7toi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A5M4AV20_9BACT A0A5M4AV20_9BACT]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acetylated glucuronoxylan is one of the most common types of hemicellulose in nature. The structure is formed by a beta-(1-->4)-linked D-xylopyranosyl (Xylp) backbone that can be substituted with an acetyl group at O-2 and O-3 positions, and alpha-(1-->2)-linked 4-O-methylglucopyranosyluronic acid (MeGlcpA). Acetyl xylan esterases (AcXE) that target mono- or doubly acetylated Xylp are well characterized; however, the previously studied AcXE from Flavobacterium johnsoniae (FjoAcXE) was the first to remove the acetyl group from 2-O-MeGlcpA-3-O-acetyl-substituted Xylp units, yet structural characteristics of these enzymes remain unspecified. Here, six homologs of FjoAcXE were produced and three crystal structures of the enzymes were solved. Two of them are complex structures, one with bound MeGlcpA and another with acetate. All homologs were confirmed to release acetate from 2-O-MeGlcpA-3-O-acetyl-substituted xylan, and the crystal structures point to key structural elements that might serve as defining features of this unclassified carbohydrate esterase family. Enzymes comprised two domains: N-terminal CBM domain and a C-terminal SGNH domain. In FjoAcXE and all studied homologs, the sequence motif around the catalytic serine is Gly-Asn-Ser-Ile (GNSI), which differs from other SGNH hydrolases. Binding by the MeGlcpA-Xylp ligand is directed by positively charged and highly conserved residues at the interface of the CBM and SGNH domains of the enzyme.
		+
		+	Elucidating Sequence and Structural Determinants of Carbohydrate Esterases for Complete Deacetylation of Substituted Xylans.,Penttinen L, Kouhi V, Faure R, Skarina T, Stogios P, Master E, Jurak E Molecules. 2022 Apr 20;27(9). pii: molecules27092655. doi:, 10.3390/molecules27092655. PMID:35566004<ref>PMID:35566004</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7toi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Prolixibacter bellariivorans]]
	[[Category: Di Leo R]]		[[Category: Di Leo R]]
	[[Category: Jurak E]]		[[Category: Jurak E]]

OCA at 09:48, 13 April 2022

OCA — Wed, 13 Apr 2022 09:48:22 GMT

←Older revision		Revision as of 09:48, 13 April 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7toi ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of carbohydrate esterase PbeAcXE, in complex with acetate==
-		+	<StructureSection load='7toi' size='340' side='right'caption='[[7toi]]' scene=''>
-	~~Authors~~: ~~Stogios~~, P.J., ~~Skarina, T~~., ~~Di Leo, R~~., ~~Jurak, E~~., ~~Master, E~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TOI FirstGlance]. <br>
-	~~Description: Crystal structure of carbohydrate esterase PbeAcXE, in complex with acetate~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7toi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7toi OCA], [https://pdbe.org/7toi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7toi RCSB], [https://www.ebi.ac.uk/pdbsum/7toi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7toi ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Di Leo, R]]	+	__TOC__
-	[[Category: Jurak, E]]	+	</StructureSection>
-	[[Category: ~~Stogios, P.J~~]]	+	[[Category: Large Structures]]
-	[[Category: Skarina, T]]	+	[[Category: Di Leo R]]
-	[[Category: ~~Master, E~~]]	+	[[Category: Jurak E]]
		+	[[Category: Master E]]
		+	[[Category: Skarina T]]
		+	[[Category: Stogios PJ]]

OCA at 05:03, 2 March 2022

OCA — Wed, 02 Mar 2022 05:03:56 GMT

←Older revision		Revision as of 05:03, 2 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7toi is ON HOLD	+	The entry 7toi is ON HOLD until Paper Publication

	Authors: Stogios, P.J., Skarina, T., Di Leo, R., Jurak, E., Master, E.		Authors: Stogios, P.J., Skarina, T., Di Leo, R., Jurak, E., Master, E.
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	Description: Crystal structure of carbohydrate esterase PbeAcXE, in complex with acetate		Description: Crystal structure of carbohydrate esterase PbeAcXE, in complex with acetate
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Stogios, P.J]]
-	[[Category: Skarina, T]]
	[[Category: Di Leo, R]]		[[Category: Di Leo, R]]
	[[Category: Jurak, E]]		[[Category: Jurak, E]]
		+	[[Category: Stogios, P.J]]
		+	[[Category: Skarina, T]]
	[[Category: Master, E]]		[[Category: Master, E]]

OCA: Protected "7toi" [edit=sysop:move=sysop]

OCA — Wed, 02 Feb 2022 05:39:08 GMT

Protected "7toi" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:39, 2 February 2022

OCA: New page: '''Unreleased structure''' The entry 7toi is ON HOLD Authors: Stogios, P.J., Skarina, T., Di Leo, R., Jurak, E., Master, E. Description: Crystal structure of carbohydrate esterase PbeA...

OCA — Wed, 02 Feb 2022 05:39:07 GMT

New page: '''Unreleased structure''' The entry 7toi is ON HOLD Authors: Stogios, P.J., Skarina, T., Di Leo, R., Jurak, E., Master, E. Description: Crystal structure of carbohydrate esterase PbeA...

New page

'''Unreleased structure'''

The entry 7toi is ON HOLD

Authors: Stogios, P.J., Skarina, T., Di Leo, R., Jurak, E., Master, E.

Description: Crystal structure of carbohydrate esterase PbeAcXE, in complex with acetate
[[Category: Unreleased Structures]]
[[Category: Stogios, P.J]]
[[Category: Skarina, T]]
[[Category: Di Leo, R]]
[[Category: Jurak, E]]
[[Category: Master, E]]