OCA at 08:52, 28 December 2022

OCA — Wed, 28 Dec 2022 08:52:40 GMT

←Older revision		Revision as of 08:52, 28 December 2022
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-	'''Unreleased structure'''

-	The ~~entry~~ 7tub is ~~ON HOLD until Paper~~ Publication	+	==The beta-tubulin folding intermediate IV==
		+	<StructureSection load='7tub' size='340' side='right'caption='[[7tub]], [[Resolution\|resolution]] 3.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7tub]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TUB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tub OCA], [https://pdbe.org/7tub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tub RCSB], [https://www.ebi.ac.uk/pdbsum/7tub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tub ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TBB5_HUMAN TBB5_HUMAN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ATP-dependent ring-shaped chaperonin TRiC/CCT is essential for cellular proteostasis. To uncover why some eukaryotic proteins can only fold with TRiC assistance, we reconstituted the folding of beta-tubulin using human prefoldin and TRiC. We find unstructured beta-tubulin is delivered by prefoldin to the open TRiC chamber followed by ATP-dependent chamber closure. Cryo-EM resolves four near-atomic-resolution structures containing progressively folded beta-tubulin intermediates within the closed TRiC chamber, culminating in native tubulin. This substrate folding pathway appears closely guided by site-specific interactions with conserved regions in the TRiC chamber. Initial electrostatic interactions between the TRiC interior wall and both the folded tubulin N domain and its C-terminal E-hook tail establish the native substrate topology, thus enabling C-domain folding. Intrinsically disordered CCT C termini within the chamber promote subsequent folding of tubulin's core and middle domains and GTP-binding. Thus, TRiC's chamber provides chemical and topological directives that shape the folding landscape of its obligate substrates.

-	~~Authors~~:	+	Structural visualization of the tubulin folding pathway directed by human chaperonin TRiC/CCT.,Gestaut D, Zhao Y, Park J, Ma B, Leitner A, Collier M, Pintilie G, Roh SH, Chiu W, Frydman J Cell. 2022 Dec 8;185(25):4770-4787.e20. doi: 10.1016/j.cell.2022.11.014. PMID:36493755<ref>PMID:36493755</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7tub" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Chiu W]]
		+	[[Category: Frydman J]]
		+	[[Category: Zhao Y]]

OCA at 04:55, 7 December 2022

OCA — Wed, 07 Dec 2022 04:55:17 GMT

←Older revision		Revision as of 04:55, 7 December 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7tub is ON HOLD	+	The entry 7tub is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7tub" [edit=sysop:move=sysop]

OCA — Wed, 09 Feb 2022 06:22:22 GMT

Protected "7tub" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:22, 9 February 2022

OCA: New page: '''Unreleased structure''' The entry 7tub is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 Feb 2022 06:22:21 GMT

New page: '''Unreleased structure''' The entry 7tub is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7tub is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7tub - Revision history

OCA at 08:52, 28 December 2022

OCA at 04:55, 7 December 2022

OCA: Protected "7tub" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7tub is ON HOLD Authors: Description: Category: Unreleased Structures