7tv8 - Revision history

OCA at 11:16, 15 November 2023

OCA — Wed, 15 Nov 2023 11:16:44 GMT

←Older revision		Revision as of 11:16, 15 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7tv8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lasioglossum_laticeps Lasioglossum laticeps]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TV8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7tv8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lasioglossum_laticeps Lasioglossum laticeps]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TV8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1VR:(3R)-3-AMINO-4-METHYLPENTANOIC+ACID'>1VR</scene>, <scene name='pdbligand=B3K:(3S)-3,7-DIAMINOHEPTANOIC+ACID'>B3K</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=XCP:(1S,2S)-2-AMINOCYCLOPENTANECARBOXYLIC+ACID'>XCP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1VR:(3R)-3-AMINO-4-METHYLPENTANOIC+ACID'>1VR</scene>, <scene name='pdbligand=B3K:(3S)-3,7-DIAMINOHEPTANOIC+ACID'>B3K</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=XCP:(1S,2S)-2-AMINOCYCLOPENTANECARBOXYLIC+ACID'>XCP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tv8 OCA], [https://pdbe.org/7tv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tv8 RCSB], [https://www.ebi.ac.uk/pdbsum/7tv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tv8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tv8 OCA], [https://pdbe.org/7tv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tv8 RCSB], [https://www.ebi.ac.uk/pdbsum/7tv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tv8 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:21, 14 June 2023

OCA — Wed, 14 Jun 2023 11:21:21 GMT

←Older revision		Revision as of 11:21, 14 June 2023
Line 3:		Line 3:
	<StructureSection load='7tv8' size='340' side='right'caption='[[7tv8]]' scene=''>		<StructureSection load='7tv8' size='340' side='right'caption='[[7tv8]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TV8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7tv8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lasioglossum_laticeps Lasioglossum laticeps]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TV8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tv8 OCA], [https://pdbe.org/7tv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tv8 RCSB], [https://www.ebi.ac.uk/pdbsum/7tv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tv8 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1VR:(3R)-3-AMINO-4-METHYLPENTANOIC+ACID'>1VR</scene>, <scene name='pdbligand=B3K:(3S)-3,7-DIAMINOHEPTANOIC+ACID'>B3K</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=XCP:(1S,2S)-2-AMINOCYCLOPENTANECARBOXYLIC+ACID'>XCP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tv8 OCA], [https://pdbe.org/7tv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tv8 RCSB], [https://www.ebi.ac.uk/pdbsum/7tv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tv8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/W5IDB3_LASLA W5IDB3_LASLA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The emergence of resistance to clinically used antibiotics by bacteria presents a significant problem in public health. Natural antimicrobial peptides (AMPs) are a valuable source of antibiotics that act by a mechanism less prone to the evolutionary development of resistance. In an effort to realize the promise of AMPs while overcoming limitations such as poor biostability, researchers have sought sequence-defined oligomers with artificial amide-based backbones that show membrane-disrupting functions similar to natural agents. Most of this precedent has focused on short peptidomimetic analogues of unstructured chains or secondary folds; however, the natural antimicrobial arsenal includes a number of small- and medium-sized proteins that act via an ordered tertiary structure. Generating proteomimetic analogues of these scaffolds poses a challenge due to the increased complexity of the target for mimicry. Here, we report the development of heterogeneous-backbone variants of lasiocepsin, a 27-residue disulfide-rich AMP found in bee venom that adopts a compact tertiary fold. Iterative cycles of design, synthesis, and biological evaluation yielded analogues of the natural domain with approximately 30 to 40% artificial backbone content, comparable antibacterial activity, reduced host cell toxicity, and improved stability to proteolytic degradation. High-resolution structures determined for several variants by NMR provide insights into the interplay among backbone composition, tertiary fold, and biological properties. Collectively, the results reported here broaden the scope of protein functional mimicry by artificial backbone analogues of tertiary folding patterns and suggest protein backbone engineering as a means to tune protein function by exerting site-specific control over protein folded structure.
		+
		+	Heterogeneous-Backbone Proteomimetic Analogues of Lasiocepsin, a Disulfide-Rich Antimicrobial Peptide with a Compact Tertiary Fold.,Cabalteja CC, Lin Q, Harmon TW, Rao SR, Di YP, Horne WS ACS Chem Biol. 2022 Apr 15;17(4):987-997. doi: 10.1021/acschembio.2c00138. Epub, 2022 Mar 15. PMID:35290019<ref>PMID:35290019</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7tv8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Lasioglossum laticeps]]
	[[Category: Cabalteja CC]]		[[Category: Cabalteja CC]]
	[[Category: Harmon TH]]		[[Category: Harmon TH]]
	[[Category: Horne WS]]		[[Category: Horne WS]]
	[[Category: Rao SR]]		[[Category: Rao SR]]

OCA at 12:53, 4 May 2022

OCA — Wed, 04 May 2022 12:53:08 GMT

←Older revision		Revision as of 12:53, 4 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7tv8 is ~~ON HOLD until Paper Publication~~	+	==Heterogeneous-backbone proteomimetic analogue of the disulfide-rich venom peptide lasiocepsin: D-Ala modified loop==
-		+	<StructureSection load='7tv8' size='340' side='right'caption='[[7tv8]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TV8 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tv8 OCA], [https://pdbe.org/7tv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tv8 RCSB], [https://www.ebi.ac.uk/pdbsum/7tv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tv8 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cabalteja CC]]
		+	[[Category: Harmon TH]]
		+	[[Category: Horne WS]]
		+	[[Category: Rao SR]]

OCA at 04:53, 9 March 2022

OCA — Wed, 09 Mar 2022 04:53:56 GMT

←Older revision		Revision as of 04:53, 9 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7tv8 is ON HOLD	+	The entry 7tv8 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7tv8" [edit=sysop:move=sysop]

OCA — Wed, 16 Feb 2022 09:09:56 GMT

Protected "7tv8" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:09, 16 February 2022

OCA: New page: '''Unreleased structure''' The entry 7tv8 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Feb 2022 09:09:55 GMT

New page: '''Unreleased structure''' The entry 7tv8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7tv8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]