OCA at 17:08, 18 October 2023

OCA — Wed, 18 Oct 2023 17:08:23 GMT

←Older revision		Revision as of 17:08, 18 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7tvn is ~~ON HOLD until Paper~~ Publication	+	==Viral AMG chitosanase V-Csn, D148N mutant==
		+	<StructureSection load='7tvn' size='340' side='right'caption='[[7tvn]], [[Resolution\|resolution]] 1.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7tvn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Unclassified_sequences Unclassified sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TVN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TVN FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tvn OCA], [https://pdbe.org/7tvn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tvn RCSB], [https://www.ebi.ac.uk/pdbsum/7tvn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tvn ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metagenomics is unearthing the previously hidden world of soil viruses. Many soil viral sequences in metagenomes contain putative auxiliary metabolic genes (AMGs) that are not associated with viral replication. Here, we establish that AMGs on soil viruses actually produce functional, active proteins. We focus on AMGs that potentially encode chitosanase enzymes that metabolize chitin - a common carbon polymer. We express and functionally screen several chitosanase genes identified from environmental metagenomes. One expressed protein showing endo-chitosanase activity (V-Csn) is crystalized and structurally characterized at ultra-high resolution, thus representing the structure of a soil viral AMG product. This structure provides details about the active site, and together with structure models determined using AlphaFold, facilitates understanding of substrate specificity and enzyme mechanism. Our findings support the hypothesis that soil viruses contribute auxiliary functions to their hosts.

-	~~Authors: Smith, C.A~~., Wu, R., Buchko, ~~G.W.~~, ~~Cort~~, ~~J.R.~~, Hofmockel, ~~K.S.~~, Jansson~~, J~~.K.	+	Structural characterization of a soil viral auxiliary metabolic gene product - a functional chitosanase.,Wu R, Smith CA, Buchko GW, Blaby IK, Paez-Espino D, Kyrpides NC, Yoshikuni Y, McDermott JE, Hofmockel KS, Cort JR, Jansson JK Nat Commun. 2022 Sep 19;13(1):5485. doi: 10.1038/s41467-022-32993-8. PMID:36123347<ref>PMID:36123347</ref>

-	~~Description: Viral AMG chitosanase V-Csn~~, ~~D148N mutant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Hofmockel, K.S~~]]	+	<div class="pdbe-citations 7tvn" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Wu, R~~]]	+	== References ==
-	[[Category: ~~Smith, C.A~~]]	+	<references/>
-	[[Category: ~~Buchko, G.W~~]]	+	__TOC__
-	[[Category: Jansson~~, J.K~~]]	+	</StructureSection>
-	[[Category: ~~Cort, J.~~R]]	+	[[Category: Large Structures]]
		+	[[Category: Unclassified sequences]]
		+	[[Category: Buchko GW]]
		+	[[Category: Cort JR]]
		+	[[Category: Hofmockel KS]]
		+	[[Category: Jansson JK]]
		+	[[Category: Smith CA]]
		+	[[Category: Wu R]]

OCA: Protected "7tvn" [edit=sysop:move=sysop]

OCA — Wed, 16 Feb 2022 09:10:43 GMT

Protected "7tvn" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:10, 16 February 2022

OCA: New page: '''Unreleased structure''' The entry 7tvn is ON HOLD until Paper Publication Authors: Smith, C.A., Wu, R., Buchko, G.W., Cort, J.R., Hofmockel, K.S., Jansson, J.K. Description: Viral A...

OCA — Wed, 16 Feb 2022 09:10:42 GMT

New page: '''Unreleased structure''' The entry 7tvn is ON HOLD until Paper Publication Authors: Smith, C.A., Wu, R., Buchko, G.W., Cort, J.R., Hofmockel, K.S., Jansson, J.K. Description: Viral A...

New page

'''Unreleased structure'''

The entry 7tvn is ON HOLD until Paper Publication

Authors: Smith, C.A., Wu, R., Buchko, G.W., Cort, J.R., Hofmockel, K.S., Jansson, J.K.

Description: Viral AMG chitosanase V-Csn, D148N mutant
[[Category: Unreleased Structures]]
[[Category: Hofmockel, K.S]]
[[Category: Wu, R]]
[[Category: Smith, C.A]]
[[Category: Buchko, G.W]]
[[Category: Jansson, J.K]]
[[Category: Cort, J.R]]

7tvn - Revision history

OCA at 17:08, 18 October 2023

OCA: Protected "7tvn" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7tvn is ON HOLD until Paper Publication Authors: Smith, C.A., Wu, R., Buchko, G.W., Cort, J.R., Hofmockel, K.S., Jansson, J.K. Description: Viral A...