OCA at 17:09, 18 October 2023

OCA — Wed, 18 Oct 2023 17:09:27 GMT

←Older revision		Revision as of 17:09, 18 October 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7tzi is ~~ON HOLD until Paper~~ Publication	+	==Structure of a pseudomurein peptide ligase type E from Methanothermobacter thermautotrophicus==
		+	<StructureSection load='7tzi' size='340' side='right'caption='[[7tzi]], [[Resolution\|resolution]] 2.91Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7tzi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TZI FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.911Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tzi OCA], [https://pdbe.org/7tzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tzi RCSB], [https://www.ebi.ac.uk/pdbsum/7tzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tzi ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A7J4MVA9_METTF A0A7J4MVA9_METTF]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Archaea have diverse cell wall types, yet none are identical to bacterial peptidoglycan (murein). Methanogens Methanobacteria and Methanopyrus possess cell walls of pseudomurein, a structural analogue of murein. Pseudomurein differs from murein in containing the unique archaeal sugar N-acetyltalosaminuronic acid instead of N-acetylmuramic acid, beta-1,3 glycosidic bonds in place of beta-1,4 bonds and only l-amino acids in the peptide cross-links. We have determined crystal structures of methanogen pseudomurein peptide ligases (termed pMurE) from Methanothermus fervidus (Mfer762) and Methanothermobacter thermautotrophicus (Mth734) that are structurally most closely related to bacterial MurE peptide ligases. The homology of the archaeal pMurE and bacterial MurE enzymes is clear both in the overall structure and at the level of each of the three domains. In addition, we identified two UDP-binding sites in Mfer762 pMurE, one at the exterior surface of the interface of the N-terminal and middle domains, and a second site at an inner surface continuous with the highly conserved interface of the three domains. Residues involved in ATP binding in MurE are conserved in pMurE, suggesting that a similar ATP-binding pocket is present at the interface of the middle and the C-terminal domains of pMurE. The presence of pMurE ligases in members of the Methanobacteriales and Methanopyrales, that are structurally related to bacterial MurE ligases, supports the idea that the biosynthetic origins of archaeal pseudomurein and bacterial peptidoglycan cell walls are evolutionarily related.

-	~~Authors: Carbone~~, V., Schofield, ~~L.R.~~, Sutherland-Smith~~, A~~.J.~~, Ronimus, R~~.S.~~, Subedi, B~~.P.	+	Structural characterisation of methanogen pseudomurein cell wall peptide ligases homologous to bacterial MurE/F murein peptide ligases.,Subedi BP, Schofield LR, Carbone V, Wolf M, Martin WF, Ronimus RS, Sutherland-Smith AJ Microbiology (Reading). 2022 Sep;168(9). doi: 10.1099/mic.0.001235. PMID:36178458<ref>PMID:36178458</ref>

-	~~Description: Structure of~~ a ~~pseudomurein peptide ligase type E from Methanothermobacter thermautotrophicus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Ronimus, R.S~~]]	+	<div class="pdbe-citations 7tzi" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Schofield, L.R~~]]	+	== References ==
-	[[Category: ~~Carbone, V~~]]	+	<references/>
-	[[Category: ~~Sutherland-Smith, A.J~~]]	+	__TOC__
-	[[Category: Subedi~~, B.P~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Methanothermobacter thermautotrophicus]]
		+	[[Category: Carbone V]]
		+	[[Category: Ronimus RS]]
		+	[[Category: Schofield LR]]
		+	[[Category: Subedi BP]]
		+	[[Category: Sutherland-Smith AJ]]

OCA at 09:29, 30 March 2022

OCA — Wed, 30 Mar 2022 09:29:10 GMT

←Older revision		Revision as of 09:29, 30 March 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7tzi is ON HOLD	+	The entry 7tzi is ON HOLD until Paper Publication

	Authors: Carbone, V., Schofield, L.R., Sutherland-Smith, A.J., Ronimus, R.S., Subedi, B.P.		Authors: Carbone, V., Schofield, L.R., Sutherland-Smith, A.J., Ronimus, R.S., Subedi, B.P.

OCA: Protected "7tzi" [edit=sysop:move=sysop]

OCA — Wed, 23 Feb 2022 06:34:38 GMT

Protected "7tzi" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:34, 23 February 2022

OCA: New page: '''Unreleased structure''' The entry 7tzi is ON HOLD Authors: Carbone, V., Schofield, L.R., Sutherland-Smith, A.J., Ronimus, R.S., Subedi, B.P. Description: Structure of a pseudomurein...

OCA — Wed, 23 Feb 2022 06:34:37 GMT

New page: '''Unreleased structure''' The entry 7tzi is ON HOLD Authors: Carbone, V., Schofield, L.R., Sutherland-Smith, A.J., Ronimus, R.S., Subedi, B.P. Description: Structure of a pseudomurein...

New page

'''Unreleased structure'''

The entry 7tzi is ON HOLD

Authors: Carbone, V., Schofield, L.R., Sutherland-Smith, A.J., Ronimus, R.S., Subedi, B.P.

Description: Structure of a pseudomurein peptide ligase type E from Methanothermobacter thermautotrophicus
[[Category: Unreleased Structures]]
[[Category: Ronimus, R.S]]
[[Category: Schofield, L.R]]
[[Category: Carbone, V]]
[[Category: Sutherland-Smith, A.J]]
[[Category: Subedi, B.P]]

7tzi - Revision history

OCA at 17:09, 18 October 2023

OCA at 09:29, 30 March 2022

OCA: Protected "7tzi" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7tzi is ON HOLD Authors: Carbone, V., Schofield, L.R., Sutherland-Smith, A.J., Ronimus, R.S., Subedi, B.P. Description: Structure of a pseudomurein...