7u0x - Revision history

OCA at 16:05, 14 March 2024

OCA — Thu, 14 Mar 2024 16:05:22 GMT

←Older revision		Revision as of 16:05, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7u0x]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U0X FirstGlance]. <br>		<table><tr><td colspan='2'>[[7u0x]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U0X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.82Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u0x OCA], [https://pdbe.org/7u0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u0x RCSB], [https://www.ebi.ac.uk/pdbsum/7u0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u0x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u0x OCA], [https://pdbe.org/7u0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u0x RCSB], [https://www.ebi.ac.uk/pdbsum/7u0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u0x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]		[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:58, 2 March 2023

OCA — Thu, 02 Mar 2023 05:58:07 GMT

←Older revision		Revision as of 05:58, 2 March 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7u0x is ~~ON HOLD until Paper Publication~~	+	==SARS-Cov2 S protein structure in complex with neutralizing monoclonal antibody 002-13==
-		+	<StructureSection load='7u0x' size='340' side='right'caption='[[7u0x]], [[Resolution\|resolution]] 3.82Å' scene=''>
-	~~Authors~~: ~~Patel~~, A., ~~Ortlund~~, E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[7u0x]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U0X FirstGlance]. <br>
-	~~Description~~: ~~SARS~~-~~Cov2 S~~ protein structure in ~~complex with neutralizing monoclonal antibody 002~~-13	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u0x OCA], [https://pdbe.org/7u0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u0x RCSB], [https://www.ebi.ac.uk/pdbsum/7u0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u0x ProSAT]</span></td></tr>
-	[[Category: Ortlund, E]]	+	</table>
-	[[Category: Patel, A]]	+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Ortlund E]]
		+	[[Category: Patel A]]

OCA at 05:01, 16 March 2022

OCA — Wed, 16 Mar 2022 05:01:19 GMT

←Older revision		Revision as of 05:01, 16 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7u0x is ON HOLD	+	The entry 7u0x is ON HOLD until Paper Publication

	Authors: Patel, A., Ortlund, E.		Authors: Patel, A., Ortlund, E.

OCA: Protected "7u0x" [edit=sysop:move=sysop]

OCA — Wed, 02 Mar 2022 05:06:48 GMT

Protected "7u0x" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:06, 2 March 2022

OCA: New page: '''Unreleased structure''' The entry 7u0x is ON HOLD Authors: Patel, A., Ortlund, E. Description: SARS-Cov2 S protein structure in complex with neutralizing monoclonal antibody 002-13 ...

OCA — Wed, 02 Mar 2022 05:06:47 GMT

New page: '''Unreleased structure''' The entry 7u0x is ON HOLD Authors: Patel, A., Ortlund, E. Description: SARS-Cov2 S protein structure in complex with neutralizing monoclonal antibody 002-13 ...

New page

'''Unreleased structure'''

The entry 7u0x is ON HOLD

Authors: Patel, A., Ortlund, E.

Description: SARS-Cov2 S protein structure in complex with neutralizing monoclonal antibody 002-13
[[Category: Unreleased Structures]]
[[Category: Ortlund, E]]
[[Category: Patel, A]]