7u6g - Revision history

OCA at 17:11, 18 October 2023

OCA — Wed, 18 Oct 2023 17:11:49 GMT

←Older revision		Revision as of 17:11, 18 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7u6g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U6G FirstGlance]. <br>		<table><tr><td colspan='2'>[[7u6g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U6G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u6g OCA], [https://pdbe.org/7u6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u6g RCSB], [https://www.ebi.ac.uk/pdbsum/7u6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u6g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u6g OCA], [https://pdbe.org/7u6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u6g RCSB], [https://www.ebi.ac.uk/pdbsum/7u6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u6g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A0H2Z6F6_PSEAB A0A0H2Z6F6_PSEAB]]	+	[https://www.uniprot.org/uniprot/A0A0H2Z6F6_PSEAB A0A0H2Z6F6_PSEAB]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:27, 7 September 2022

OCA — Wed, 07 Sep 2022 19:27:37 GMT

←Older revision		Revision as of 19:27, 7 September 2022
Line 1:		Line 1:

	==Structure of PQS Response Protein PqsE(E182W,E280A) Variant==		==Structure of PQS Response Protein PqsE(E182W,E280A) Variant==
-	<StructureSection load='7u6g' size='340' side='right'caption='[[7u6g]]' scene=''>	+	<StructureSection load='7u6g' size='340' side='right'caption='[[7u6g]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U6G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7u6g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U6G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u6g OCA], [https://pdbe.org/7u6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u6g RCSB], [https://www.ebi.ac.uk/pdbsum/7u6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u6g ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u6g OCA], [https://pdbe.org/7u6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u6g RCSB], [https://www.ebi.ac.uk/pdbsum/7u6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u6g ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A0H2Z6F6_PSEAB A0A0H2Z6F6_PSEAB]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The opportunistic pathogen Pseudomonas aeruginosa causes antibiotic-resistant, nosocomial infections in immuno-compromised individuals and is a high priority for antimicrobial development. Key to pathogenicity in P. aeruginosa are biofilm formation and virulence factor production. Both traits are controlled by the cell-to-cell communication process called quorum sensing (QS). QS involves the synthesis, release, and population-wide detection of signal molecules called autoinducers. We previously reported that the activity of the RhlR QS transcription factor depends on a protein-protein interaction with the hydrolase, PqsE, and PqsE catalytic activity is dispensable for this interaction. Nonetheless, the PqsE-RhlR interaction could be disrupted by the substitution of an active site glutamate residue with tryptophan [PqsE(E182W)]. Here, we show that disruption of the PqsE-RhlR interaction via either the E182W change or alteration of PqsE surface residues that are essential for the interaction with RhlR attenuates P. aeruginosa infection in a murine host. We use crystallography to characterize the conformational changes induced by the PqsE(E182W) substitution to define the mechanism underlying disruption of the PqsE-RhlR interaction. A loop rearrangement that repositions the E280 residue in PqsE(E182W) is responsible for the loss of interaction. We verify the implications garnered from the PqsE(E182W) structure using mutagenic, biochemical, and additional structural analyses. We present the next generation of molecules targeting the PqsE active site, including a structure of the tightest binding of these compounds, BB584, in complex with PqsE. The findings presented here provide insights into drug discovery against P. aeruginosa with PqsE as the target.
		+
		+	The PqsE Active Site as a Target for Small Molecule Antimicrobial Agents against Pseudomonas aeruginosa.,Taylor IR, Jeffrey PD, Moustafa DA, Goldberg JB, Bassler BL Biochemistry. 2022 Aug 19. doi: 10.1021/acs.biochem.2c00334. PMID:35985643<ref>PMID:35985643</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7u6g" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
	[[Category: Bassler BL]]		[[Category: Bassler BL]]
	[[Category: Jeffrey PD]]		[[Category: Jeffrey PD]]
	[[Category: Taylor IR]]		[[Category: Taylor IR]]

OCA at 06:37, 31 August 2022

OCA — Wed, 31 Aug 2022 06:37:14 GMT

←Older revision		Revision as of 06:37, 31 August 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7u6g ~~is ON HOLD until Paper Publication~~	+	==Structure of PQS Response Protein PqsE(E182W,E280A) Variant==
-		+	<StructureSection load='7u6g' size='340' side='right'caption='[[7u6g]]' scene=''>
-	~~Authors~~: ~~Jeffrey~~, P.D., ~~Taylor~~, I.R., ~~Bassler~~, B.L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U6G FirstGlance]. <br>
-	~~Description: Structure of PQS Response Protein PqsE(E182W,E280A) Variant~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u6g OCA], [https://pdbe.org/7u6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u6g RCSB], [https://www.ebi.ac.uk/pdbsum/7u6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u6g ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Taylor, I.R~~]]	+	__TOC__
-	[[Category: ~~Bassler, B.L~~]]	+	</StructureSection>
-	[[Category: ~~Jeffrey, P.D~~]]	+	[[Category: Large Structures]]
		+	[[Category: Bassler BL]]
		+	[[Category: Jeffrey PD]]
		+	[[Category: Taylor IR]]

OCA at 09:02, 6 April 2022

OCA — Wed, 06 Apr 2022 09:02:05 GMT

←Older revision		Revision as of 09:02, 6 April 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7u6g is ON HOLD	+	The entry 7u6g is ON HOLD until Paper Publication

	Authors: Jeffrey, P.D., Taylor, I.R., Bassler, B.L.		Authors: Jeffrey, P.D., Taylor, I.R., Bassler, B.L.
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Taylor, I.R]]		[[Category: Taylor, I.R]]
-	[[Category: Jeffrey, P.D]]
	[[Category: Bassler, B.L]]		[[Category: Bassler, B.L]]
		+	[[Category: Jeffrey, P.D]]

OCA: Protected "7u6g" [edit=sysop:move=sysop]

OCA — Wed, 16 Mar 2022 05:02:20 GMT

Protected "7u6g" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:02, 16 March 2022

OCA: New page: '''Unreleased structure''' The entry 7u6g is ON HOLD Authors: Jeffrey, P.D., Taylor, I.R., Bassler, B.L. Description: Structure of PQS Response Protein PqsE(E182W,E280A) Variant [[Cate...

OCA — Wed, 16 Mar 2022 05:02:19 GMT

New page: '''Unreleased structure''' The entry 7u6g is ON HOLD Authors: Jeffrey, P.D., Taylor, I.R., Bassler, B.L. Description: Structure of PQS Response Protein PqsE(E182W,E280A) Variant [[Cate...

New page

'''Unreleased structure'''

The entry 7u6g is ON HOLD

Authors: Jeffrey, P.D., Taylor, I.R., Bassler, B.L.

Description: Structure of PQS Response Protein PqsE(E182W,E280A) Variant
[[Category: Unreleased Structures]]
[[Category: Taylor, I.R]]
[[Category: Jeffrey, P.D]]
[[Category: Bassler, B.L]]