OCA at 07:34, 11 January 2023

OCA — Wed, 11 Jan 2023 07:34:33 GMT

←Older revision		Revision as of 07:34, 11 January 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7u6v is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of Shiga toxin 2 in complex with the native ribosomal P-stalk==
		+	<StructureSection load='7u6v' size='340' side='right'caption='[[7u6v]], [[Resolution\|resolution]] 4.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7u6v]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Shigella_dysenteriae Shigella dysenteriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U6V FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u6v OCA], [https://pdbe.org/7u6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u6v RCSB], [https://www.ebi.ac.uk/pdbsum/7u6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u6v ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G8GWP6_9CAUD G8GWP6_9CAUD]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Shiga toxin 2a (Stx2a) is the virulence factor of enterohemorrhagic Escherichia coli. The catalytic A1 subunit of Stx2a (Stx2A1) interacts with the ribosomal P-stalk for loading onto the ribosome and depurination of the sarcin-ricin loop, which halts protein synthesis. Because of the intrinsic flexibility of the P-stalk, a structure of the Stx2a-P-stalk complex is currently unknown. We demonstrated that the native P-stalk pentamer binds to Stx2a with nanomolar affinity, and we employed cryo-EM to determine a structure of the 72 kDa Stx2a complexed with the P-stalk. The structure identifies Stx2A1 residues involved in binding and reveals that Stx2a is anchored to the P-stalk via only the last six amino acids from the C-terminal domain of a single P-protein. For the first time, the cryo-EM structure shows the loop connecting Stx2A1 and Stx2A2, which is critical for activation of the toxin. Our principal component analysis of the cryo-EM data reveals the intrinsic dynamics of the Stx2a-P-stalk interaction, including conformational changes in the P-stalk binding site occurring upon complex formation. Our computational analysis unveils the propensity for structural rearrangements within the C-terminal domain, with its C-terminal six amino acids transitioning from a random coil to an alpha-helix upon binding to Stx2a. In conclusion, our cryo-EM structure sheds new light into the dynamics of the Stx2a-P-stalk interaction and indicates that the binding interface between Stx2a and the P-stalk is the potential target for drug discovery.

-	~~Authors~~:	+	Cryo-EM structure of Shiga toxin 2 in complex with the native ribosomal P-stalk reveals residues involved in the binding interaction.,Kulczyk AW, Sorzano COS, Grela P, Tchorzewski M, Tumer NE, Li XP J Biol Chem. 2022 Dec 15;299(1):102795. doi: 10.1016/j.jbc.2022.102795. PMID:36528064<ref>PMID:36528064</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7u6v" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Shigella dysenteriae]]
		+	[[Category: Kulczyk AW]]

OCA at 09:02, 6 April 2022

OCA — Wed, 06 Apr 2022 09:02:08 GMT

←Older revision		Revision as of 09:02, 6 April 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7u6v is ON HOLD	+	The entry 7u6v is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7u6v" [edit=sysop:move=sysop]

OCA — Wed, 16 Mar 2022 05:03:12 GMT

Protected "7u6v" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:03, 16 March 2022

OCA: New page: '''Unreleased structure''' The entry 7u6v is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Mar 2022 05:03:11 GMT

New page: '''Unreleased structure''' The entry 7u6v is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7u6v is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7u6v - Revision history

OCA at 07:34, 11 January 2023

OCA at 09:02, 6 April 2022

OCA: Protected "7u6v" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7u6v is ON HOLD Authors: Description: Category: Unreleased Structures