OCA at 17:12, 18 October 2023

OCA — Wed, 18 Oct 2023 17:12:53 GMT

←Older revision		Revision as of 17:12, 18 October 2023
Line 1:		Line 1:

-	====	+	==Crystal structure of chimeric hemagglutinin cH15/3 in complex with broad protective antibody 31.a.83==
-	<StructureSection load='7u8l' size='340' side='right'caption='[[7u8l]]' scene=''>	+	<StructureSection load='7u8l' size='340' side='right'caption='[[7u8l]], [[Resolution\|resolution]] 4.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7u8l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Influenza_A_virus Influenza A virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U8L FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u8l OCA], [https://pdbe.org/7u8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u8l RCSB], [https://www.ebi.ac.uk/pdbsum/7u8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u8l ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.56Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u8l OCA], [https://pdbe.org/7u8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u8l RCSB], [https://www.ebi.ac.uk/pdbsum/7u8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u8l ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A385F4V1_9INFA A0A385F4V1_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324][https://www.uniprot.org/uniprot/HEMA_I56A1 HEMA_I56A1] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[HAMAP-Rule:MF_04072][https://www.uniprot.org/uniprot/A0A0G2YG46_9INFA A0A0G2YG46_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[HAMAP-Rule:MF_04072] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Influenza virus hemagglutinin (HA) has been the primary target for influenza vaccine development. Broadly protective antibodies targeting conserved regions of the HA unlock the possibility of generating universal influenza immunity. Two group 2 influenza A chimeric HAs, cH4/3 and cH15/3, were previously designed to elicit antibodies to the conserved HA stem. Here, we show by X-ray crystallography and negative-stain electron microscopy that a broadly protective antistem antibody can stably bind to cH4/3 and cH15/3 HAs, thereby validating their potential as universal vaccine immunogens. Furthermore, flexibility was observed in the head domain of the chimeric HA structures, suggesting that antibodies could also potentially interact with the head interface epitope. Our structural and binding studies demonstrated that a broadly protective antihead trimeric interface antibody could indeed target the more open head domain of the cH15/3 HA trimer. Thus, in addition to inducing broadly protective antibodies against the conserved HA stem, chimeric HAs may also be able to elicit antibodies against the conserved trimer interface in the HA head domain, thereby increasing the vaccine efficacy.
		+
		+	Influenza chimeric hemagglutinin structures in complex with broadly protective antibodies to the stem and trimer interface.,Zhu X, Han J, Sun W, Puente-Massaguer E, Yu W, Palese P, Krammer F, Ward AB, Wilson IA Proc Natl Acad Sci U S A. 2022 May 24;119(21):e2200821119. doi: , 10.1073/pnas.2200821119. Epub 2022 May 20. PMID:35594401<ref>PMID:35594401</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7u8l" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Influenza A virus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Wilson IA]]
		+	[[Category: Zhu X]]

OCA at 05:30, 8 June 2022

OCA — Wed, 08 Jun 2022 05:30:27 GMT

←Older revision		Revision as of 05:30, 8 June 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7u8l ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7u8l' size='340' side='right'caption='[[7u8l]]' scene=''>
-	~~Authors~~: ~~Zhu, X~~.~~, Wilson, I~~.A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal structure of chimeric hemagglutinin cH15~~/~~3 in complex with broad protective antibody 31~~.a.83	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u8l OCA], [https://pdbe.org/7u8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u8l RCSB], [https://www.ebi.ac.uk/pdbsum/7u8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u8l ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Wilson~~, I.A]]	+	__TOC__
-	[[Category: ~~Zhu, X~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7u8l" [edit=sysop:move=sysop]

OCA — Wed, 16 Mar 2022 05:05:41 GMT

Protected "7u8l" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:05, 16 March 2022

OCA: New page: '''Unreleased structure''' The entry 7u8l is ON HOLD until Paper Publication Authors: Zhu, X., Wilson, I.A. Description: Crystal structure of chimeric hemagglutinin cH15/3 in complex w...

OCA — Wed, 16 Mar 2022 05:05:40 GMT

New page: '''Unreleased structure''' The entry 7u8l is ON HOLD until Paper Publication Authors: Zhu, X., Wilson, I.A. Description: Crystal structure of chimeric hemagglutinin cH15/3 in complex w...

New page

'''Unreleased structure'''

The entry 7u8l is ON HOLD until Paper Publication

Authors: Zhu, X., Wilson, I.A.

Description: Crystal structure of chimeric hemagglutinin cH15/3 in complex with broad protective antibody 31.a.83
[[Category: Unreleased Structures]]
[[Category: Wilson, I.A]]
[[Category: Zhu, X]]

7u8l - Revision history

OCA at 17:12, 18 October 2023

OCA at 05:30, 8 June 2022

OCA: Protected "7u8l" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7u8l is ON HOLD until Paper Publication Authors: Zhu, X., Wilson, I.A. Description: Crystal structure of chimeric hemagglutinin cH15/3 in complex w...