7u8o - Revision history

OCA at 09:38, 14 February 2024

OCA — Wed, 14 Feb 2024 09:38:14 GMT

←Older revision		Revision as of 09:38, 14 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7u8o]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U8O FirstGlance]. <br>		<table><tr><td colspan='2'>[[7u8o]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U8O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u8o OCA], [https://pdbe.org/7u8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u8o RCSB], [https://www.ebi.ac.uk/pdbsum/7u8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u8o ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.5Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u8o OCA], [https://pdbe.org/7u8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u8o RCSB], [https://www.ebi.ac.uk/pdbsum/7u8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u8o ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/VATA_PIG VATA_PIG]] Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity). May play a role in neurite development and synaptic connectivity (By similarity).[UniProtKB:P38606]	+	[https://www.uniprot.org/uniprot/VATA_PIG VATA_PIG] Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity). May play a role in neurite development and synaptic connectivity (By similarity).[UniProtKB:P38606]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	V-ATPases are rotary proton pumps that serve as signaling hubs with numerous protein binding partners. CryoEM with exhaustive focused classification allowed detection of endogenous proteins associated with porcine kidney V-ATPase. An extra C subunit was found in approximately 3% of complexes, whereas approximately 1.6% of complexes bound mEAK-7, a protein with proposed roles in dauer formation in nematodes and mTOR signaling in mammals. High-resolution cryoEM of porcine kidney V-ATPase with recombinant mEAK-7 showed that mEAK-7's TLDc domain interacts with V-ATPase's stator, whereas its C-terminal alpha helix binds V-ATPase's rotor. This crosslink would be expected to inhibit rotary catalysis. However, unlike the yeast TLDc protein Oxr1p, exogenous mEAK-7 does not inhibit V-ATPase and mEAK-7 overexpression in cells does not alter lysosomal or phagosomal pH. Instead, cryoEM suggests that the mEAK-7:V-ATPase interaction is disrupted by ATP-induced rotation of the rotor. Comparison of Oxr1p and mEAK-7 binding explains this difference. These results show that V-ATPase binding by TLDc domain proteins can lead to effects ranging from strong inhibition to formation of labile interactions that are sensitive to the enzyme's activity.	+

-	CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7.,Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL Life Sci Alliance. 2022 Jul 6;5(11). pii: 5/11/e202201527. doi:, 10.26508/lsa.202201527. Print 2022 Nov. PMID:35794005<ref>PMID:35794005</ref>	+	==See Also==
-		+	*[[ATPase 3D structures\|ATPase 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 7u8o" style~~=~~"background-color:#fffaf0;"></div>~~	+
-	~~== References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 03:55, 8 September 2022

OCA — Thu, 08 Sep 2022 03:55:16 GMT

←Older revision		Revision as of 03:55, 8 September 2022
Line 1:		Line 1:

-	====	+	==Structure of porcine V-ATPase with mEAK7 and SidK, Rotary state 2==
-	<StructureSection load='7u8o' size='340' side='right'caption='[[7u8o]]' scene=''>	+	<StructureSection load='7u8o' size='340' side='right'caption='[[7u8o]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7u8o]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U8O FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u8o OCA], [https://pdbe.org/7u8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u8o RCSB], [https://www.ebi.ac.uk/pdbsum/7u8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u8o ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u8o OCA], [https://pdbe.org/7u8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u8o RCSB], [https://www.ebi.ac.uk/pdbsum/7u8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u8o ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/VATA_PIG VATA_PIG]] Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity). May play a role in neurite development and synaptic connectivity (By similarity).[UniProtKB:P38606]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	V-ATPases are rotary proton pumps that serve as signaling hubs with numerous protein binding partners. CryoEM with exhaustive focused classification allowed detection of endogenous proteins associated with porcine kidney V-ATPase. An extra C subunit was found in approximately 3% of complexes, whereas approximately 1.6% of complexes bound mEAK-7, a protein with proposed roles in dauer formation in nematodes and mTOR signaling in mammals. High-resolution cryoEM of porcine kidney V-ATPase with recombinant mEAK-7 showed that mEAK-7's TLDc domain interacts with V-ATPase's stator, whereas its C-terminal alpha helix binds V-ATPase's rotor. This crosslink would be expected to inhibit rotary catalysis. However, unlike the yeast TLDc protein Oxr1p, exogenous mEAK-7 does not inhibit V-ATPase and mEAK-7 overexpression in cells does not alter lysosomal or phagosomal pH. Instead, cryoEM suggests that the mEAK-7:V-ATPase interaction is disrupted by ATP-induced rotation of the rotor. Comparison of Oxr1p and mEAK-7 binding explains this difference. These results show that V-ATPase binding by TLDc domain proteins can lead to effects ranging from strong inhibition to formation of labile interactions that are sensitive to the enzyme's activity.
		+
		+	CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7.,Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL Life Sci Alliance. 2022 Jul 6;5(11). pii: 5/11/e202201527. doi:, 10.26508/lsa.202201527. Print 2022 Nov. PMID:35794005<ref>PMID:35794005</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7u8o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Legionella pneumophila]]
		+	[[Category: Sus scrofa]]
		+	[[Category: Keon KA]]
		+	[[Category: Tan YZ]]

OCA at 16:14, 6 July 2022

OCA — Wed, 06 Jul 2022 16:14:35 GMT

←Older revision		Revision as of 16:14, 6 July 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7u8o ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7u8o' size='340' side='right'caption='[[7u8o]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u8o OCA], [https://pdbe.org/7u8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u8o RCSB], [https://www.ebi.ac.uk/pdbsum/7u8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u8o ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:48, 23 March 2022

OCA — Wed, 23 Mar 2022 09:48:11 GMT

←Older revision		Revision as of 09:48, 23 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7u8o is ON HOLD	+	The entry 7u8o is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7u8o" [edit=sysop:move=sysop]

OCA — Wed, 16 Mar 2022 05:05:47 GMT

Protected "7u8o" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:05, 16 March 2022

OCA: New page: '''Unreleased structure''' The entry 7u8o is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Mar 2022 05:05:47 GMT

New page: '''Unreleased structure''' The entry 7u8o is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7u8o is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]