7uch - Revision history

OCA at 17:13, 18 October 2023

OCA — Wed, 18 Oct 2023 17:13:56 GMT

←Older revision		Revision as of 17:13, 18 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7uch]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorena_bouillonii Moorena bouillonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCH FirstGlance]. <br>		<table><tr><td colspan='2'>[[7uch]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorena_bouillonii Moorena bouillonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MU6:dimethylpropanedioic+acid'>MU6</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.18Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MU6:dimethylpropanedioic+acid'>MU6</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uch OCA], [https://pdbe.org/7uch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uch RCSB], [https://www.ebi.ac.uk/pdbsum/7uch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uch ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uch OCA], [https://pdbe.org/7uch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uch RCSB], [https://www.ebi.ac.uk/pdbsum/7uch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uch ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A1U7N2Z8_9CYAN A0A1U7N2Z8_9CYAN]]	+	[https://www.uniprot.org/uniprot/A0A1U7N2Z8_9CYAN A0A1U7N2Z8_9CYAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:49, 7 September 2022

OCA — Wed, 07 Sep 2022 19:49:50 GMT

←Older revision		Revision as of 19:49, 7 September 2022
Line 1:		Line 1:

	==AprA Methyltransferase 1 - GNAT in complex with Mn2+ , SAM, and Di-methyl-malonate==		==AprA Methyltransferase 1 - GNAT in complex with Mn2+ , SAM, and Di-methyl-malonate==
-	<StructureSection load='7uch' size='340' side='right'caption='[[7uch]]' scene=''>	+	<StructureSection load='7uch' size='340' side='right'caption='[[7uch]], [[Resolution\|resolution]] 2.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7uch]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorena_bouillonii Moorena bouillonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uch OCA], [https://pdbe.org/7uch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uch RCSB], [https://www.ebi.ac.uk/pdbsum/7uch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uch ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MU6:dimethylpropanedioic+acid'>MU6</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uch OCA], [https://pdbe.org/7uch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uch RCSB], [https://www.ebi.ac.uk/pdbsum/7uch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uch ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A1U7N2Z8_9CYAN A0A1U7N2Z8_9CYAN]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Installation of methyl groups can significantly improve the binding of small-molecule drugs to protein targets; however, site-selective methylation often presents a significant synthetic challenge. Metal- and S-adenosyl-methionine (SAM)-dependent methyltransferases (MTs) in natural-product biosynthetic pathways are powerful enzymatic tools for selective or chemically challenging C-methylation reactions. Each of these MTs selectively catalyzes one or two methyl transfer reactions. Crystal structures and biochemical assays of the Mn(2+)-dependent monomethyltransferase from the saxitoxin biosynthetic pathway (SxtA MT) revealed the structural basis for control of methylation extent. The SxtA monomethyltransferase was converted to a dimethyltransferase by modification of the metal binding site, addition of an active site base, and an amino acid substitution to provide space in the substrate pocket for two methyl substituents. A reciprocal change converted a related dimethyltransferase into a monomethyltransferase, supporting our hypothesis that steric hindrance can prevent a second methylation event. A novel understanding of MTs will accelerate the development of MT-based catalysts and MT engineering for use in small-molecule synthesis.
		+
		+	Structural Basis for Control of Methylation Extent in Polyketide Synthase Metal-Dependent C-Methyltransferases.,Lao Y, Skiba MA, Chun SW, Narayan ARH, Smith JL ACS Chem Biol. 2022 Aug 19;17(8):2088-2098. doi: 10.1021/acschembio.2c00085. Epub, 2022 May 20. PMID:35594521<ref>PMID:35594521</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7uch" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Moorena bouillonii]]
	[[Category: Lao Y]]		[[Category: Lao Y]]
	[[Category: Skiba MA]]		[[Category: Skiba MA]]
	[[Category: Smith JL]]		[[Category: Smith JL]]

OCA at 06:44, 31 August 2022

OCA — Wed, 31 Aug 2022 06:44:31 GMT

←Older revision		Revision as of 06:44, 31 August 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7uch is ON HOLD until Paper Publication~~	+	==AprA Methyltransferase 1 - GNAT in complex with Mn2+ , SAM, and Di-methyl-malonate==
-		+	<StructureSection load='7uch' size='340' side='right'caption='[[7uch]]' scene=''>
-	~~Authors: Skiba, M.A., Lao, Y., Smith, J.L.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCH FirstGlance]. <br>
-	~~Description:~~ AprA Methyltransferase 1 -GNAT in complex with Mn2+ , SAM, and Di-methyl-malonate	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uch OCA], [https://pdbe.org/7uch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uch RCSB], [https://www.ebi.ac.uk/pdbsum/7uch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uch ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Lao, Y]]	+	__TOC__
-	[[Category: ~~Smith, J.L~~]]	+	</StructureSection>
-	[[Category: ~~Skiba, M.A~~]]	+	[[Category: Large Structures]]
		+	[[Category: Lao Y]]
		+	[[Category: Skiba MA]]
		+	[[Category: Smith JL]]

OCA at 01:33, 21 April 2022

OCA — Thu, 21 Apr 2022 01:33:35 GMT

←Older revision		Revision as of 01:33, 21 April 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uch is ON HOLD	+	The entry 7uch is ON HOLD until Paper Publication

	Authors: Skiba, M.A., Lao, Y., Smith, J.L.		Authors: Skiba, M.A., Lao, Y., Smith, J.L.

OCA: Protected "7uch" [edit=sysop:move=sysop]

OCA — Wed, 23 Mar 2022 09:53:44 GMT

Protected "7uch" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:53, 23 March 2022

OCA: New page: '''Unreleased structure''' The entry 7uch is ON HOLD Authors: Skiba, M.A., Lao, Y., Smith, J.L. Description: AprA Methyltransferase 1 -GNAT in complex with Mn2+ , SAM, and Di-methyl-ma...

OCA — Wed, 23 Mar 2022 09:53:43 GMT

New page: '''Unreleased structure''' The entry 7uch is ON HOLD Authors: Skiba, M.A., Lao, Y., Smith, J.L. Description: AprA Methyltransferase 1 -GNAT in complex with Mn2+ , SAM, and Di-methyl-ma...

New page

'''Unreleased structure'''

The entry 7uch is ON HOLD

Authors: Skiba, M.A., Lao, Y., Smith, J.L.

Description: AprA Methyltransferase 1 -GNAT in complex with Mn2+ , SAM, and Di-methyl-malonate
[[Category: Unreleased Structures]]
[[Category: Lao, Y]]
[[Category: Smith, J.L]]
[[Category: Skiba, M.A]]