7udt - Revision history

OCA at 03:19, 8 September 2022

OCA — Thu, 08 Sep 2022 03:19:38 GMT

←Older revision		Revision as of 03:19, 8 September 2022
Line 1:		Line 1:

-	====	+	==cryo-EM structure of the rigor state wild type myosin-15-F-actin complex (symmetry expansion and re-centering)==
-	<StructureSection load='7udt' size='340' side='right'caption='[[7udt]]' scene=''>	+	<StructureSection load='7udt' size='340' side='right'caption='[[7udt]], [[Resolution\|resolution]] 3.17Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7udt]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UDT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7udt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7udt OCA], [https://pdbe.org/7udt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7udt RCSB], [https://www.ebi.ac.uk/pdbsum/7udt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7udt ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7udt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7udt OCA], [https://pdbe.org/7udt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7udt RCSB], [https://www.ebi.ac.uk/pdbsum/7udt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7udt ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/ACTS_CHICK ACTS_CHICK]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The motor protein myosin-15 is necessary for the development and maintenance of mechanosensory stereocilia, and mutations in myosin-15 cause hereditary deafness. In addition to transporting actin regulatory machinery to stereocilia tips, myosin-15 directly nucleates actin filament ("F-actin") assembly, which is disrupted by a progressive hearing loss mutation (p.D1647G, "jordan"). Here, we present cryo-electron microscopy structures of myosin-15 bound to F-actin, providing a framework for interpreting the impacts of deafness mutations on motor activity and actin nucleation. Rigor myosin-15 evokes conformational changes in F-actin yet maintains flexibility in actin's D-loop, which mediates inter-subunit contacts, while the jordan mutant locks the D-loop in a single conformation. Adenosine diphosphate-bound myosin-15 also locks the D-loop, which correspondingly blunts actin-polymerization stimulation. We propose myosin-15 enhances polymerization by bridging actin protomers, regulating nucleation efficiency by modulating actin's structural plasticity in a myosin nucleotide state-dependent manner. This tunable regulation of actin polymerization could be harnessed to precisely control stereocilium height.
		+
		+	Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia.,Gong R, Jiang F, Moreland ZG, Reynolds MJ, de Los Reyes SE, Gurel P, Shams A, Heidings JB, Bowl MR, Bird JE, Alushin GM Sci Adv. 2022 Jul 22;8(29):eabl4733. doi: 10.1126/sciadv.abl4733. Epub 2022 Jul, 20. PMID:35857845<ref>PMID:35857845</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7udt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Actin 3D structures\|Actin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Alushin GM]]
		+	[[Category: Gong R]]
		+	[[Category: Reynolds MJ]]

OCA at 04:53, 3 August 2022

OCA — Wed, 03 Aug 2022 04:53:59 GMT

←Older revision		Revision as of 04:53, 3 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7udt ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7udt' size='340' side='right'caption='[[7udt]]' scene=''>
-	~~Authors~~: ~~Gong, R~~.~~, Reynolds, M~~.J.~~, Alushin, G~~.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~cryo-EM structure of the rigor state wild type myosin-15-F-actin complex (symmetry expansion and re-centering)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7udt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7udt OCA], [https://pdbe.org/7udt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7udt RCSB], [https://www.ebi.ac.uk/pdbsum/7udt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7udt ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Reynolds, M~~.J]]	+	__TOC__
-	[[Category: ~~Alushin, G.M~~]]	+	</StructureSection>
-	[[Category: ~~Gong, R~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7udt" [edit=sysop:move=sysop]

OCA — Wed, 30 Mar 2022 09:33:04 GMT

Protected "7udt" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:33, 30 March 2022

OCA: New page: '''Unreleased structure''' The entry 7udt is ON HOLD Authors: Gong, R., Reynolds, M.J., Alushin, G.M. Description: cryo-EM structure of the rigor state wild type myosin-15-F-actin comp...

OCA — Wed, 30 Mar 2022 09:33:02 GMT

New page: '''Unreleased structure''' The entry 7udt is ON HOLD Authors: Gong, R., Reynolds, M.J., Alushin, G.M. Description: cryo-EM structure of the rigor state wild type myosin-15-F-actin comp...

New page

'''Unreleased structure'''

The entry 7udt is ON HOLD

Authors: Gong, R., Reynolds, M.J., Alushin, G.M.

Description: cryo-EM structure of the rigor state wild type myosin-15-F-actin complex (symmetry expansion and re-centering)
[[Category: Unreleased Structures]]
[[Category: Reynolds, M.J]]
[[Category: Alushin, G.M]]
[[Category: Gong, R]]