7ufi - Revision history

OCA at 18:58, 7 September 2022

OCA — Wed, 07 Sep 2022 18:58:02 GMT

←Older revision		Revision as of 18:58, 7 September 2022
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ufi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ufi OCA], [https://pdbe.org/7ufi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ufi RCSB], [https://www.ebi.ac.uk/pdbsum/7ufi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ufi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ufi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ufi OCA], [https://pdbe.org/7ufi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ufi RCSB], [https://www.ebi.ac.uk/pdbsum/7ufi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ufi ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial transposons are pervasive mobile genetic elements that use distinct DNA-binding proteins for horizontal transmission. For example, Escherichia coli Tn7 homes to a specific attachment site using TnsD(1), whereas CRISPR-associated transposons use type I or type V Cas effectors to insert downstream of target sites specified by guide RNAs(2,3). Despite this targeting diversity, transposition invariably requires TnsB, a DDE-family transposase that catalyses DNA excision and insertion, and TnsC, a AAA+ ATPase that is thought to communicate between transposase and targeting proteins(4). How TnsC mediates this communication and thereby regulates transposition fidelity has remained unclear. Here we use chromatin immunoprecipitation with sequencing to monitor in vivo formation of the type I-F RNA-guided transpososome, enabling us to resolve distinct protein recruitment events before integration. DNA targeting by the TniQ-Cascade complex is surprisingly promiscuous-hundreds of genomic off-target sites are sampled, but only a subset of those sites is licensed for TnsC and TnsB recruitment, revealing a crucial proofreading checkpoint. To advance the mechanistic understanding of interactions responsible for transpososome assembly, we determined structures of TnsC using cryogenic electron microscopy and found that ATP binding drives the formation of heptameric rings that thread DNA through the central pore, thereby positioning the substrate for downstream integration. Collectively, our results highlight the molecular specificity imparted by consecutive factor binding to genomic target sites during RNA-guided transposition, and provide a structural roadmap to guide future engineering efforts.
		+
		+	Selective TnsC recruitment enhances the fidelity of RNA-guided transposition.,Hoffmann FT, Kim M, Beh LY, Wang J, Vo PLH, Gelsinger DR, George JT, Acree C, Mohabir JT, Fernandez IS, Sternberg SH Nature. 2022 Aug 24. pii: 10.1038/s41586-022-05059-4. doi:, 10.1038/s41586-022-05059-4. PMID:36002573<ref>PMID:36002573</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ufi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:49, 7 September 2022

OCA — Wed, 07 Sep 2022 16:49:02 GMT

←Older revision		Revision as of 16:49, 7 September 2022
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-	====	+	==VchTnsC AAA+ ATPase with DNA, single heptamer==
-	<StructureSection load='7ufi' size='340' side='right'caption='[[7ufi]]' scene=''>	+	<StructureSection load='7ufi' size='340' side='right'caption='[[7ufi]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ufi]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UFI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ufi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ufi OCA], [https://pdbe.org/7ufi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ufi RCSB], [https://www.ebi.ac.uk/pdbsum/7ufi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ufi ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ufi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ufi OCA], [https://pdbe.org/7ufi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ufi RCSB], [https://www.ebi.ac.uk/pdbsum/7ufi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ufi ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Vibrio cholerae]]
		+	[[Category: Fernandez IS]]
		+	[[Category: Sternberg SH]]

OCA at 05:30, 8 June 2022

OCA — Wed, 08 Jun 2022 05:30:38 GMT

←Older revision		Revision as of 05:30, 8 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7ufi ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7ufi' size='340' side='right'caption='[[7ufi]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ufi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ufi OCA], [https://pdbe.org/7ufi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ufi RCSB], [https://www.ebi.ac.uk/pdbsum/7ufi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ufi ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 10:23, 27 April 2022

OCA — Wed, 27 Apr 2022 10:23:49 GMT

←Older revision		Revision as of 10:23, 27 April 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ufi is ON HOLD	+	The entry 7ufi is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7ufi" [edit=sysop:move=sysop]

OCA — Wed, 30 Mar 2022 09:35:39 GMT

Protected "7ufi" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:35, 30 March 2022

OCA: New page: '''Unreleased structure''' The entry 7ufi is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 30 Mar 2022 09:35:38 GMT

New page: '''Unreleased structure''' The entry 7ufi is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ufi is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]