7uhn - Revision history

OCA at 09:53, 25 October 2023

OCA — Wed, 25 Oct 2023 09:53:05 GMT

←Older revision		Revision as of 09:53, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7uhn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stenotrophomonas_maltophilia_K279a Stenotrophomonas maltophilia K279a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UHN FirstGlance]. <br>		<table><tr><td colspan='2'>[[7uhn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stenotrophomonas_maltophilia_K279a Stenotrophomonas maltophilia K279a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UHN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XQP:(2R)-2-[(R)-carboxy{[(2R)-2-carboxy-2-(4-hydroxyphenyl)acetyl]amino}methoxymethyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic+acid'>XQP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XQP:(2R)-2-[(R)-carboxy{[(2R)-2-carboxy-2-(4-hydroxyphenyl)acetyl]amino}methoxymethyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic+acid'>XQP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uhn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uhn OCA], [https://pdbe.org/7uhn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uhn RCSB], [https://www.ebi.ac.uk/pdbsum/7uhn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uhn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uhn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uhn OCA], [https://pdbe.org/7uhn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uhn RCSB], [https://www.ebi.ac.uk/pdbsum/7uhn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uhn ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:57, 8 February 2023

OCA — Wed, 08 Feb 2023 06:57:41 GMT

←Older revision		Revision as of 06:57, 8 February 2023
Line 1:		Line 1:

	==Time-Resolved Structure of Metallo Beta-Lactamase L1 in a Complex with Cleaved Moxalactam (300 ms Snapshot)==		==Time-Resolved Structure of Metallo Beta-Lactamase L1 in a Complex with Cleaved Moxalactam (300 ms Snapshot)==
-	<StructureSection load='7uhn' size='340' side='right'caption='[[7uhn]]' scene=''>	+	<StructureSection load='7uhn' size='340' side='right'caption='[[7uhn]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UHN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7uhn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stenotrophomonas_maltophilia_K279a Stenotrophomonas maltophilia K279a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UHN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uhn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uhn OCA], [https://pdbe.org/7uhn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uhn RCSB], [https://www.ebi.ac.uk/pdbsum/7uhn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uhn ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XQP:(2R)-2-[(R)-carboxy{[(2R)-2-carboxy-2-(4-hydroxyphenyl)acetyl]amino}methoxymethyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic+acid'>XQP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uhn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uhn OCA], [https://pdbe.org/7uhn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uhn RCSB], [https://www.ebi.ac.uk/pdbsum/7uhn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uhn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B2FTM1_STRMK B2FTM1_STRMK]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serial x-ray crystallography can uncover binding events, and subsequent chemical conversions occurring during enzymatic reaction. Here, we reveal the structure, binding and cleavage of moxalactam antibiotic bound to L1 metallo-beta-lactamase (MBL) from Stenotrophomonas maltophilia. Using time-resolved serial synchrotron crystallography, we show the time course of beta-lactam hydrolysis and determine ten snapshots (20, 40, 60, 80, 100, 150, 300, 500, 2000 and 4000 ms) at 2.20 A resolution. The reaction is initiated by laser pulse releasing Zn(2+) ions from a UV-labile photocage. Two metal ions bind to the active site, followed by binding of moxalactam and the intact beta-lactam ring is observed for 100 ms after photolysis. Cleavage of beta-lactam is detected at 150 ms and the ligand is significantly displaced. The reaction product adjusts its conformation reaching steady state at 2000 ms corresponding to the relaxed state of the enzyme. Only small changes are observed in the positions of Zn(2+) ions and the active site residues. Mechanistic details captured here can be generalized to other MBLs.
		+
		+	Time-resolved beta-lactam cleavage by L1 metallo-beta-lactamase.,Wilamowski M, Sherrell DA, Kim Y, Lavens A, Henning RW, Lazarski K, Shigemoto A, Endres M, Maltseva N, Babnigg G, Burdette SC, Srajer V, Joachimiak A Nat Commun. 2022 Nov 30;13(1):7379. doi: 10.1038/s41467-022-35029-3. PMID:36450742<ref>PMID:36450742</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7uhn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Stenotrophomonas maltophilia K279a]]
	[[Category: Babnigg G]]		[[Category: Babnigg G]]
	[[Category: Endres M]]		[[Category: Endres M]]

OCA at 09:48, 13 April 2022

OCA — Wed, 13 Apr 2022 09:48:48 GMT

←Older revision		Revision as of 09:48, 13 April 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7uhn is ON HOLD~~	+	==Time-Resolved Structure of Metallo Beta-Lactamase L1 in a Complex with Cleaved Moxalactam (300 ms Snapshot)==
-		+	<StructureSection load='7uhn' size='340' side='right'caption='[[7uhn]]' scene=''>
-	Authors: Wilamowski, M., Kim, Y., Sherrell, D.A., Lavens, A., Henning, R., Maltseva, N., Endres, M., Babnigg, G., Srajer, V., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID)	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UHN FirstGlance]. <br>
-	~~Description:~~ Time-Resolved Structure of Metallo Beta-Lactamase L1 in a Complex with Cleaved Moxalactam (300 ms Snapshot)	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uhn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uhn OCA], [https://pdbe.org/7uhn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uhn RCSB], [https://www.ebi.ac.uk/pdbsum/7uhn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uhn ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Wilamowski~~, M]]	+	__TOC__
-	[[Category: ~~Srajer, V~~]]	+	</StructureSection>
-	[[Category: ~~Center For Structural Genomics Of Infectious Diseases (Csgid)~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Babnigg, G~~]]	+	[[Category: Babnigg G]]
-	[[Category: ~~Maltseva, N~~]]	+	[[Category: Endres M]]
-	[[Category: Kim, Y]]	+	[[Category: Henning R]]
-	[[Category: Lavens, A]]	+	[[Category: Joachimiak A]]
-	[[Category: ~~Sherrell, D.A~~]]	+	[[Category: Kim Y]]
-	[[Category: ~~Joachimiak, A~~]]	+	[[Category: Lavens A]]
-	[[Category: ~~Endres, M~~]]	+	[[Category: Maltseva N]]
-	[[Category: ~~Henning, R~~]]	+	[[Category: Sherrell DA]]
		+	[[Category: Srajer V]]
		+	[[Category: Wilamowski M]]

OCA: Protected "7uhn" [edit=sysop:move=sysop]

OCA — Wed, 06 Apr 2022 09:04:39 GMT

Protected "7uhn" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:04, 6 April 2022

OCA: New page: '''Unreleased structure''' The entry 7uhn is ON HOLD Authors: Wilamowski, M., Kim, Y., Sherrell, D.A., Lavens, A., Henning, R., Maltseva, N., Endres, M., Babnigg, G., Srajer, V., Joachi...

OCA — Wed, 06 Apr 2022 09:04:38 GMT

New page: '''Unreleased structure''' The entry 7uhn is ON HOLD Authors: Wilamowski, M., Kim, Y., Sherrell, D.A., Lavens, A., Henning, R., Maltseva, N., Endres, M., Babnigg, G., Srajer, V., Joachi...

New page

'''Unreleased structure'''

The entry 7uhn is ON HOLD

Authors: Wilamowski, M., Kim, Y., Sherrell, D.A., Lavens, A., Henning, R., Maltseva, N., Endres, M., Babnigg, G., Srajer, V., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: Time-Resolved Structure of Metallo Beta-Lactamase L1 in a Complex with Cleaved Moxalactam (300 ms Snapshot)
[[Category: Unreleased Structures]]
[[Category: Wilamowski, M]]
[[Category: Srajer, V]]
[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]
[[Category: Babnigg, G]]
[[Category: Maltseva, N]]
[[Category: Kim, Y]]
[[Category: Lavens, A]]
[[Category: Sherrell, D.A]]
[[Category: Joachimiak, A]]
[[Category: Endres, M]]
[[Category: Henning, R]]