OCA at 05:58, 2 March 2023

OCA — Thu, 02 Mar 2023 05:58:13 GMT

←Older revision		Revision as of 05:58, 2 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7uik is ~~ON HOLD~~	+	==Mediator-PIC Early (Tail A + Upstream DNA & Activator)==
		+	<StructureSection load='7uik' size='340' side='right'caption='[[7uik]], [[Resolution\|resolution]] 7.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7uik]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UIK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uik OCA], [https://pdbe.org/7uik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uik RCSB], [https://www.ebi.ac.uk/pdbsum/7uik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uik ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Most eukaryotic promoter regions are divergently transcribed. As the RNA polymerase II pre-initiation complex (PIC) is intrinsically asymmetric and responsible for transcription in a single direction, it is unknown how divergent transcription arises. Here, the Saccharomyces cerevisiae Mediator complexed with a PIC (Med-PIC) was assembled on a divergent promoter and analyzed by cryoelectron microscopy. The structure reveals two distinct Med-PICs forming a dimer through the Mediator tail module, induced by a homodimeric activator protein localized near the dimerization interface. The tail dimer is associated with approximately 80-bp upstream DNA, such that two flanking core promoter regions are positioned and oriented in a suitable form for PIC assembly in opposite directions. Also, cryoelectron tomography visualized the progress of the PIC assembly on the two core promoter regions, providing direct evidence for the role of the Med-PIC dimer in divergent transcription.

-	~~Authors~~:	+	Structural basis of a transcription pre-initiation complex on a divergent promoter.,Gorbea Colon JJ, Palao L 3rd, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K Mol Cell. 2023 Jan 26:S1097-2765(23)00031-X. doi: 10.1016/j.molcel.2023.01.011. PMID:36731470<ref>PMID:36731470</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7uik" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae S288C]]
		+	[[Category: Chen S-F]]
		+	[[Category: Gorbea Colon JJ]]
		+	[[Category: Murakami K]]
		+	[[Category: Tsai KL]]

OCA: Protected "7uik" [edit=sysop:move=sysop]

OCA — Wed, 06 Apr 2022 09:06:08 GMT

Protected "7uik" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:06, 6 April 2022

OCA: New page: '''Unreleased structure''' The entry 7uik is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 Apr 2022 09:06:07 GMT

New page: '''Unreleased structure''' The entry 7uik is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7uik is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7uik - Revision history

OCA at 05:58, 2 March 2023

OCA: Protected "7uik" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7uik is ON HOLD Authors: Description: Category: Unreleased Structures