OCA at 07:07, 18 May 2023

OCA — Thu, 18 May 2023 07:07:47 GMT

←Older revision		Revision as of 07:07, 18 May 2023
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	==Thiol-disulfide oxidoreductase TsdA from Corynebacterium diphtheriae==		==Thiol-disulfide oxidoreductase TsdA from Corynebacterium diphtheriae==
-	<StructureSection load='7unn' size='340' side='right'caption='[[7unn]]' scene=''>	+	<StructureSection load='7unn' size='340' side='right'caption='[[7unn]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UNN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UNN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7unn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UNN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UNN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7unn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7unn OCA], [https://pdbe.org/7unn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7unn RCSB], [https://www.ebi.ac.uk/pdbsum/7unn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7unn ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7unn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7unn OCA], [https://pdbe.org/7unn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7unn RCSB], [https://www.ebi.ac.uk/pdbsum/7unn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7unn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6NJK4_CORDI Q6NJK4_CORDI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In many gram-positive Actinobacteria, including Actinomyces oris and Corynebacterium matruchotii, the conserved thiol-disulfide oxidoreductase MdbA that catalyzes oxidative folding of exported proteins is essential for bacterial viability by an unidentified mechanism. Intriguingly, in Corynebacterium diphtheriae, the deletion of mdbA blocks cell growth only at 37 degrees C but not at 30 degrees C, suggesting the presence of alternative oxidoreductase enzyme(s). By isolating spontaneous thermotolerant revertants of the mdbA mutant at 37 degrees C, we obtained genetic suppressors, all mapped to a single T-to-G mutation within the promoter region of tsdA, causing its elevated expression. Strikingly, increased expression of tsdA-via suppressor mutations or a constitutive promoter-rescues the pilus assembly and toxin production defects of this mutant, hence compensating for the loss of mdbA. Structural, genetic, and biochemical analyses demonstrated TsdA is a membrane-tethered thiol-disulfide oxidoreductase with a conserved CxxC motif that can substitute for MdbA in mediating oxidative folding of pilin and toxin substrates. Together with our observation that tsdA expression is upregulated at nonpermissive temperature (40 degrees C) in wild-type cells, we posit that TsdA has evolved as a compensatory thiol-disulfide oxidoreductase that safeguards oxidative protein folding in C. diphtheriae against thermal stress.
		+
		+	A cryptic oxidoreductase safeguards oxidative protein folding in Corynebacterium diphtheriae.,Reardon-Robinson ME, Nguyen MT, Sanchez BC, Osipiuk J, Ruckert C, Chang C, Chen B, Nagvekar R, Joachimiak A, Tauch A, Das A, Ton-That H Proc Natl Acad Sci U S A. 2023 Feb 21;120(8):e2208675120. doi: , 10.1073/pnas.2208675120. Epub 2023 Feb 14. PMID:36787356<ref>PMID:36787356</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7unn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Corynebacterium diphtheriae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Joachimiak A]]		[[Category: Joachimiak A]]

OCA: Protected "7unn" [edit=sysop:move=sysop]

OCA — Thu, 21 Apr 2022 02:57:48 GMT

Protected "7unn" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:57, 21 April 2022

OCA: New page: ==Thiol-disulfide oxidoreductase TsdA from Corynebacterium diphtheriae== == Structural highlights ==
OCA — Thu, 21 Apr 2022 02:57:47 GMT

New page: ==Thiol-disulfide oxidoreductase TsdA from Corynebacterium diphtheriae== <StructureSection load='7unn' size='340' side='right'caption='7unn' scene=''> == Structural highlights == <tab...

New page

==Thiol-disulfide oxidoreductase TsdA from Corynebacterium diphtheriae==
<StructureSection load='7unn' size='340' side='right'caption='[[7unn]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UNN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UNN FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7unn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7unn OCA], [https://pdbe.org/7unn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7unn RCSB], [https://www.ebi.ac.uk/pdbsum/7unn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7unn ProSAT]</span></td></tr>
</table>
TOC
</StructureSection>
[[Category: Large Structures]]
[[Category: Joachimiak A]]
[[Category: Nguyen MT]]
[[Category: Osipiuk J]]
[[Category: Reardon-Robinson M]]
[[Category: Sanchez B]]
[[Category: Ton-That H]]

7unn - Revision history

OCA at 07:07, 18 May 2023

OCA: Protected "7unn" [edit=sysop:move=sysop]