OCA at 06:16, 28 September 2022

OCA — Wed, 28 Sep 2022 06:16:53 GMT

←Older revision		Revision as of 06:16, 28 September 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7uo5 is ~~ON HOLD until Paper~~ Publication	+	==E.coli RNaseP Holoenzyme with Mg2+==
		+	<StructureSection load='7uo5' size='340' side='right'caption='[[7uo5]], [[Resolution\|resolution]] 3.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7uo5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UO5 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uo5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uo5 OCA], [https://pdbe.org/7uo5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uo5 RCSB], [https://www.ebi.ac.uk/pdbsum/7uo5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uo5 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/C3SLK7_ECOLX C3SLK7_ECOLX]] RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.[ARBA:ARBA00002663][HAMAP-Rule:MF_00227]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Binding of precursor tRNAs (ptRNAs) by bacterial ribonuclease P (RNase P) involves an encounter complex (ES) that isomerizes to a catalytic conformation (ES). However, the structures of intermediates and the conformational changes that occur during binding are poorly understood. Here, we show that pairing between the 5' leader and 3'RCCA extending the acceptor stem of ptRNA inhibits ES formation. Cryo-electron microscopy single particle analysis reveals a dynamic enzyme that becomes ordered upon formation of ES* in which extended acceptor stem pairing is unwound. Comparisons of structures with alternative ptRNAs reveals that once unwinding is completed RNase P primarily uses stacking interactions and shape complementarity to accommodate alternative sequences at its cleavage site. Our study reveals active site interactions and conformational changes that drive molecular recognition by RNase P and lays the foundation for understanding how binding interactions are linked to helix unwinding and catalysis.

-	~~Authors: Huang~~, W., Taylor, D.J.	+	Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P.,Zhu J, Huang W, Zhao J, Huynh L, Taylor DJ, Harris ME Nat Commun. 2022 Aug 31;13(1):5120. doi: 10.1038/s41467-022-32843-7. PMID:36045135<ref>PMID:36045135</ref>

-	~~Description: E~~.coli ~~RNaseP Holoenzyme with Mg2+~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Huang, W]]	+	<div class="pdbe-citations 7uo5" style="background-color:#fffaf0;"></div>
-	[[Category: Taylor~~, D.J~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Huang W]]
		+	[[Category: Taylor DJ]]

OCA at 09:00, 18 May 2022

OCA — Wed, 18 May 2022 09:00:43 GMT

←Older revision		Revision as of 09:00, 18 May 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uo5 is ON HOLD	+	The entry 7uo5 is ON HOLD until Paper Publication

	Authors: Huang, W., Taylor, D.J.		Authors: Huang, W., Taylor, D.J.

OCA: Protected "7uo5" [edit=sysop:move=sysop]

OCA — Thu, 21 Apr 2022 01:36:36 GMT

Protected "7uo5" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:36, 21 April 2022

OCA: New page: '''Unreleased structure''' The entry 7uo5 is ON HOLD Authors: Huang, W., Taylor, D.J. Description: E.coli RNaseP Holoenzyme with Mg2+ Category: Unreleased Structures [[Category: Hu...

OCA — Thu, 21 Apr 2022 01:36:35 GMT

New page: '''Unreleased structure''' The entry 7uo5 is ON HOLD Authors: Huang, W., Taylor, D.J. Description: E.coli RNaseP Holoenzyme with Mg2+ Category: Unreleased Structures [[Category: Hu...

New page

'''Unreleased structure'''

The entry 7uo5 is ON HOLD

Authors: Huang, W., Taylor, D.J.

Description: E.coli RNaseP Holoenzyme with Mg2+
[[Category: Unreleased Structures]]
[[Category: Huang, W]]
[[Category: Taylor, D.J]]

7uo5 - Revision history

OCA at 06:16, 28 September 2022

OCA at 09:00, 18 May 2022

OCA: Protected "7uo5" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7uo5 is ON HOLD Authors: Huang, W., Taylor, D.J. Description: E.coli RNaseP Holoenzyme with Mg2+ Category: Unreleased Structures [[Category: Hu...