7uon - Revision history

OCA at 09:55, 25 October 2023

OCA — Wed, 25 Oct 2023 09:55:31 GMT

←Older revision		Revision as of 09:55, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7uon]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UON FirstGlance]. <br>		<table><tr><td colspan='2'>[[7uon]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UON FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.35Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uon OCA], [https://pdbe.org/7uon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uon RCSB], [https://www.ebi.ac.uk/pdbsum/7uon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uon ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uon OCA], [https://pdbe.org/7uon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uon RCSB], [https://www.ebi.ac.uk/pdbsum/7uon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uon ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:30, 25 January 2023

OCA — Wed, 25 Jan 2023 06:30:27 GMT

←Older revision		Revision as of 06:30, 25 January 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7uon is ~~ON HOLD until Paper~~ Publication	+	==CTX-M-14 Y105W mutant==
		+	<StructureSection load='7uon' size='340' side='right'caption='[[7uon]], [[Resolution\|resolution]] 1.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7uon]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UON FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uon OCA], [https://pdbe.org/7uon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uon RCSB], [https://www.ebi.ac.uk/pdbsum/7uon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uon ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/H6UQI0_ECOLX H6UQI0_ECOLX]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CTX-M beta-lactamases are prevalent antibiotic resistance enzymes and are notable for their ability to rapidly hydrolyze the extended-spectrum cephalosporin, cefotaxime. We hypothesized that the active site sequence requirements of CTX-M-mediated hydrolysis differ between classes of beta-lactam antibiotics. Accordingly, we use codon randomization, antibiotic selection, and deep sequencing to determine the CTX-M active-site residues required for hydrolysis of cefotaxime and the penicillin, ampicillin. The study reveals positions required for hydrolysis of all beta-lactams, as well as residues controlling substrate specificity. Further, CTX-M enzymes poorly hydrolyze the extended-spectrum cephalosporin, ceftazidime. We further show that the sequence requirements for ceftazidime hydrolysis follow those of cefotaxime, with the exception that key active-site omega loop residues are not required, and may be detrimental, for ceftazidime hydrolysis. These results provide insights into cephalosporin hydrolysis and demonstrate that changes to the active-site omega loop are likely required for the evolution of CTX-M-mediated ceftazidime resistance.

-	~~Authors~~:	+	Mapping the determinants of catalysis and substrate specificity of the antibiotic resistance enzyme CTX-M beta-lactamase.,Judge A, Hu L, Sankaran B, Van Riper J, Venkataram Prasad BV, Palzkill T Commun Biol. 2023 Jan 12;6(1):35. doi: 10.1038/s42003-023-04422-z. PMID:36635385<ref>PMID:36635385</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7uon" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Hu L]]
		+	[[Category: Judge A]]
		+	[[Category: Palzkill T]]
		+	[[Category: Prasad BVV]]
		+	[[Category: Sankaran B]]
		+	[[Category: Van Riper J]]

OCA at 15:17, 7 September 2022

OCA — Wed, 07 Sep 2022 15:17:15 GMT

←Older revision		Revision as of 15:17, 7 September 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uon is ON HOLD	+	The entry 7uon is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 03:40, 10 August 2022

OCA — Wed, 10 Aug 2022 03:40:39 GMT

←Older revision		Revision as of 03:40, 10 August 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uon is ON HOLD ~~until Paper Publication~~	+	The entry 7uon is ON HOLD

	Authors:		Authors:

OCA at 10:25, 27 April 2022

OCA — Wed, 27 Apr 2022 10:25:24 GMT

←Older revision		Revision as of 10:25, 27 April 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uon is ON HOLD	+	The entry 7uon is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7uon" [edit=sysop:move=sysop]

OCA — Thu, 21 Apr 2022 01:37:34 GMT

Protected "7uon" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:37, 21 April 2022

OCA: New page: '''Unreleased structure''' The entry 7uon is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 21 Apr 2022 01:37:33 GMT

New page: '''Unreleased structure''' The entry 7uon is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7uon is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]