7ury - Revision history

OCA at 09:56, 25 October 2023

OCA — Wed, 25 Oct 2023 09:56:26 GMT

←Older revision		Revision as of 09:56, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7ury]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7URY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7URY FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ury]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7URY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7URY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.64Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ury FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ury OCA], [https://pdbe.org/7ury PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ury RCSB], [https://www.ebi.ac.uk/pdbsum/7ury PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ury ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ury FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ury OCA], [https://pdbe.org/7ury PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ury RCSB], [https://www.ebi.ac.uk/pdbsum/7ury PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ury ProSAT]</span></td></tr>
	</table>		</table>

OCA at 04:12, 25 May 2023

OCA — Thu, 25 May 2023 04:12:35 GMT

←Older revision		Revision as of 04:12, 25 May 2023
Line 1:		Line 1:

	==Tetradecameric hub domain of CaMKII beta==		==Tetradecameric hub domain of CaMKII beta==
-	<StructureSection load='7ury' size='340' side='right'caption='[[7ury]]' scene=''>	+	<StructureSection load='7ury' size='340' side='right'caption='[[7ury]], [[Resolution\|resolution]] 2.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7URY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7URY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ury]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7URY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7URY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ury FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ury OCA], [https://pdbe.org/7ury PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ury RCSB], [https://www.ebi.ac.uk/pdbsum/7ury PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ury ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ury FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ury OCA], [https://pdbe.org/7ury PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ury RCSB], [https://www.ebi.ac.uk/pdbsum/7ury PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ury ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/KCC2B_HUMAN KCC2B_HUMAN] Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.<ref>PMID:16690701</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins are long chains of amino acid residues that perform a myriad of functions in living organisms, including enzymatic reactions, signalling, and maintaining structural integrity. Protein function is determined directly by the protein structure. X-ray crystallography is the primary technique for determining the 3D structure of proteins, and facilitates understanding the effects of protein structure on function. The first step towards structure determination is crystallizing the protein of interest. We have developed a centrifugally-actuated microfluidic device that incorporates the fluid handling and metering necessary for protein crystallization. Liquid handling takes advantage of surface forces to control fluid flow and enable metering, without the need for any fluidic or pump connections. Our approach requires only the simple steps of pipetting the crystallization reagents into the device followed by either spinning or shaking to set up counter-diffusive protein crystallization trials. The use of thin, UV-curable polymers with a high level of X-ray transparency allows for in situ X-ray crystallography, eliminating the manual handling of fragile protein crystals and streamlining the process of protein structure analysis. We demonstrate the utility of our device using hen egg white lysozyme as a model system, followed by the crystallization and in situ, room temperature structural analysis of the hub domain of calcium-calmodulin dependent kinase II (CaMKIIbeta).
		+
		+	Polymer-based microfluidic device for on-chip counter-diffusive crystallization and in situ X-ray crystallography at room temperature.,Saha S, Ozden C, Samkutty A, Russi S, Cohen A, Stratton MM, Perry SL Lab Chip. 2023 Apr 12;23(8):2075-2090. doi: 10.1039/d2lc01194h. PMID:36942575<ref>PMID:36942575</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ury" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Calcium/calmodulin dependent protein kinase 3D structures\|Calcium/calmodulin dependent protein kinase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Garman SC]]		[[Category: Garman SC]]

OCA at 06:21, 12 May 2022

OCA — Thu, 12 May 2022 06:21:08 GMT

←Older revision		Revision as of 06:21, 12 May 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ury ~~is ON HOLD~~	+	==Tetradecameric hub domain of CaMKII beta==
-		+	<StructureSection load='7ury' size='340' side='right'caption='[[7ury]]' scene=''>
-	~~Authors~~: ~~Ozden, C~~., ~~Saha, S~~., ~~Samkutty, A~~., ~~Stratton, M~~.M., ~~Garman, S~~.C., ~~Perry, S~~.L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7URY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7URY FirstGlance]. <br>
-	~~Description: Tetradecameric hub domain of CaMKII beta~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ury FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ury OCA], [https://pdbe.org/7ury PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ury RCSB], [https://www.ebi.ac.uk/pdbsum/7ury PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ury ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Perry, S.L~~]]	+	__TOC__
-	[[Category: Ozden, C]]	+	</StructureSection>
-	[[Category: ~~Saha, S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Samkutty, A~~]]	+	[[Category: Garman SC]]
-	[[Category: ~~Garman, S.C~~]]	+	[[Category: Ozden C]]
-	[[Category: Stratton~~, M.M~~]]	+	[[Category: Perry SL]]
		+	[[Category: Saha S]]
		+	[[Category: Samkutty A]]
		+	[[Category: Stratton MM]]

OCA: Protected "7ury" [edit=sysop:move=sysop]

OCA — Wed, 04 May 2022 11:32:00 GMT

Protected "7ury" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:32, 4 May 2022

OCA: New page: '''Unreleased structure''' The entry 7ury is ON HOLD Authors: Ozden, C., Saha, S., Samkutty, A., Stratton, M.M., Garman, S.C., Perry, S.L. Description: Tetradecameric hub domain of CaM...

OCA — Wed, 04 May 2022 11:31:59 GMT

New page: '''Unreleased structure''' The entry 7ury is ON HOLD Authors: Ozden, C., Saha, S., Samkutty, A., Stratton, M.M., Garman, S.C., Perry, S.L. Description: Tetradecameric hub domain of CaM...

New page

'''Unreleased structure'''

The entry 7ury is ON HOLD

Authors: Ozden, C., Saha, S., Samkutty, A., Stratton, M.M., Garman, S.C., Perry, S.L.

Description: Tetradecameric hub domain of CaMKII beta
[[Category: Unreleased Structures]]
[[Category: Perry, S.L]]
[[Category: Ozden, C]]
[[Category: Saha, S]]
[[Category: Samkutty, A]]
[[Category: Garman, S.C]]
[[Category: Stratton, M.M]]