7ute - Revision history

OCA at 09:39, 14 February 2024

OCA — Wed, 14 Feb 2024 09:39:13 GMT

←Older revision		Revision as of 09:39, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='7ute' size='340' side='right'caption='[[7ute]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='7ute' size='340' side='right'caption='[[7ute]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ute]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UTE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ute]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UTE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ute FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ute OCA], [https://pdbe.org/7ute PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ute RCSB], [https://www.ebi.ac.uk/pdbsum/7ute PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ute ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ute FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ute OCA], [https://pdbe.org/7ute PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ute RCSB], [https://www.ebi.ac.uk/pdbsum/7ute PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ute ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q9YFF4_AERPE Q9YFF4_AERPE]
-	Biocatalytic carbene transfer from diazo compounds is a versatile strategy in asymmetric synthesis. However, the limited pool of stable diazo compounds constrains the variety of accessible products. To overcome this restriction, we have engineered variants of Aeropyrum pernix protoglobin (ApePgb) that use diazirines as carbene precursors. While the enhanced stability of diazirines relative to their diazo isomers enables access to a diverse array of carbenes, they have previously resisted catalytic activation. Our engineered ApePgb variants represent the first example of catalysts for selective carbene transfer from these species at room temperature. The structure of an ApePgb variant, determined by microcrystal electron diffraction (MicroED), reveals that evolution has enhanced access to the heme active site to facilitate this new-to-nature catalysis. Using readily prepared aryl diazirines as model substrates, we demonstrate the application of these highly stable carbene precursors in biocatalytic cyclopropanation, N-H insertion, and Si-H insertion reactions.	+
-		+
-	~~Biocatalytic Carbene Transfer Using Diazirines.,Porter NJ, Danelius E, Gonen T, Arnold FH J Am Chem Soc. 2022 May 13. doi~~: ~~10.1021~~/~~jacs.2c02723. PMID:35561334<ref>PMID:35561334<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 7ute" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aeropyrum pernix]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Danelius, E]]	+	[[Category: Danelius E]]
-	[[Category: Gonen, T]]	+	[[Category: Gonen T]]
-	[[Category: Unge~~, J T]]~~	+	[[Category: Unge JT]]
-	~~[[Category: Heme]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Microed]]~~	+
-	~~[[Category: Protoglobin~~]]	+

OCA at 05:37, 8 June 2022

OCA — Wed, 08 Jun 2022 05:37:27 GMT

←Older revision		Revision as of 05:37, 8 June 2022
Line 1:		Line 1:

	==MicroED structure of Aeropyrum pernix protoglobin mutant==		==MicroED structure of Aeropyrum pernix protoglobin mutant==
-	<StructureSection load='7ute' size='340' side='right'caption='[[7ute]]' scene=''>	+	<StructureSection load='7ute' size='340' side='right'caption='[[7ute]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UTE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ute]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UTE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ute FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ute OCA], [https://pdbe.org/7ute PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ute RCSB], [https://www.ebi.ac.uk/pdbsum/7ute PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ute ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ute FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ute OCA], [https://pdbe.org/7ute PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ute RCSB], [https://www.ebi.ac.uk/pdbsum/7ute PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ute ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Biocatalytic carbene transfer from diazo compounds is a versatile strategy in asymmetric synthesis. However, the limited pool of stable diazo compounds constrains the variety of accessible products. To overcome this restriction, we have engineered variants of Aeropyrum pernix protoglobin (ApePgb) that use diazirines as carbene precursors. While the enhanced stability of diazirines relative to their diazo isomers enables access to a diverse array of carbenes, they have previously resisted catalytic activation. Our engineered ApePgb variants represent the first example of catalysts for selective carbene transfer from these species at room temperature. The structure of an ApePgb variant, determined by microcrystal electron diffraction (MicroED), reveals that evolution has enhanced access to the heme active site to facilitate this new-to-nature catalysis. Using readily prepared aryl diazirines as model substrates, we demonstrate the application of these highly stable carbene precursors in biocatalytic cyclopropanation, N-H insertion, and Si-H insertion reactions.
		+
		+	Biocatalytic Carbene Transfer Using Diazirines.,Porter NJ, Danelius E, Gonen T, Arnold FH J Am Chem Soc. 2022 May 13. doi: 10.1021/jacs.2c02723. PMID:35561334<ref>PMID:35561334</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ute" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Danelius E]]	+	[[Category: Danelius, E]]
-	[[Category: Gonen T]]	+	[[Category: Gonen, T]]
-	[[Category: Unge JT]]	+	[[Category: Unge, J T]]
		+	[[Category: Heme]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Microed]]
		+	[[Category: Protoglobin]]

OCA at 07:59, 25 May 2022

OCA — Wed, 25 May 2022 07:59:51 GMT

←Older revision		Revision as of 07:59, 25 May 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ute is ~~ON HOLD until Paper Publication~~	+	==MicroED structure of Aeropyrum pernix protoglobin mutant==
-		+	<StructureSection load='7ute' size='340' side='right'caption='[[7ute]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UTE FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ute FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ute OCA], [https://pdbe.org/7ute PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ute RCSB], [https://www.ebi.ac.uk/pdbsum/7ute PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ute ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Danelius E]]
		+	[[Category: Gonen T]]
		+	[[Category: Unge JT]]

OCA at 09:01, 18 May 2022

OCA — Wed, 18 May 2022 09:01:46 GMT

←Older revision		Revision as of 09:01, 18 May 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ute is ON HOLD	+	The entry 7ute is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7ute" [edit=sysop:move=sysop]

OCA — Wed, 04 May 2022 11:34:25 GMT

Protected "7ute" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:34, 4 May 2022

OCA: New page: '''Unreleased structure''' The entry 7ute is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 04 May 2022 11:34:23 GMT

New page: '''Unreleased structure''' The entry 7ute is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ute is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]