OCA at 10:06, 24 November 2022

OCA — Thu, 24 Nov 2022 10:06:57 GMT

←Older revision		Revision as of 10:06, 24 November 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7uvk is ~~ON HOLD until Paper~~ Publication	+	==G. haemolysans IgA1 protease==
		+	<StructureSection load='7uvk' size='340' side='right'caption='[[7uvk]], [[Resolution\|resolution]] 3.28Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7uvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gemella_haemolysans Gemella haemolysans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UVK FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uvk OCA], [https://pdbe.org/7uvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uvk RCSB], [https://www.ebi.ac.uk/pdbsum/7uvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uvk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C5NYF3_9BACL C5NYF3_9BACL]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mucosal adaptive immune response is dependent on the production of IgA antibodies and particularly IgA1, yet opportunistic bacteria have evolved mechanisms to specifically block this response by producing IgA1 proteases (IgA1Ps). Our lab was the first to describe the structures of a metal-dependent IgA1P (metallo-IgA1P) produced from Gram-positive Streptococcus pneumoniae both in the absence and presence of its IgA1 substrate through cryo-EM single particle reconstructions. This prior study revealed an active-site gating mechanism reliant on substrate-induced conformational changes to the enzyme that begged the question of whether such a mechanism is conserved among the wider Gram-positive metallo-IgA1P subfamily of virulence factors. Here, we used cryo-EM to characterize the metallo-IgA1P of a more distantly related family member from Gemella haemolysans, an emerging opportunistic pathogen implicated in meningitis, endocarditis, and more recently bacteremia in the elderly. While the substrate-free structures of these two metallo-IgA1Ps exhibit differences in the relative starting positions of the domain responsible for gating substrate, the enzymes have similar domain orientations when bound to IgA1. Together with biochemical studies that indicate these metallo-IgA1Ps have similar binding affinities and activities, these data indicate that metallo-IgA1P binding requires the specific IgA1 substrate to open the enzymes for access to their active site and thus, largely conform to an "induced fit" model.

-	~~Authors: Eisenmesser~~, E~~.Z.~~, ~~Zheng~~, H.	+	A substrate-induced gating mechanism is conserved among Gram-positive IgA1 metalloproteases.,Redzic JS, Rahkola J, Tran N, Holyoak T, Lee E, Martin-Galiano AJ, Meyer N, Zheng H, Eisenmesser E Commun Biol. 2022 Nov 7;5(1):1190. doi: 10.1038/s42003-022-04173-3. PMID:36336763<ref>PMID:36336763</ref>

-	~~Description: G~~. haemolysans ~~IgA1 protease~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Zheng, H~~]]	+	<div class="pdbe-citations 7uvk" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Eisenmesser, E.Z~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gemella haemolysans]]
		+	[[Category: Large Structures]]
		+	[[Category: Eisenmesser EZ]]
		+	[[Category: Zheng H]]

OCA at 03:50, 15 June 2022

OCA — Wed, 15 Jun 2022 03:50:51 GMT

←Older revision		Revision as of 03:50, 15 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uvk is ON HOLD	+	The entry 7uvk is ON HOLD until Paper Publication

	Authors: Eisenmesser, E.Z., Zheng, H.		Authors: Eisenmesser, E.Z., Zheng, H.

OCA: Protected "7uvk" [edit=sysop:move=sysop]

OCA — Thu, 12 May 2022 04:48:25 GMT

Protected "7uvk" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:48, 12 May 2022

OCA: New page: '''Unreleased structure''' The entry 7uvk is ON HOLD Authors: Eisenmesser, E.Z., Zheng, H. Description: G. haemolysans IgA1 protease Category: Unreleased Structures [[Category: Zhe...

OCA — Thu, 12 May 2022 04:48:23 GMT

New page: '''Unreleased structure''' The entry 7uvk is ON HOLD Authors: Eisenmesser, E.Z., Zheng, H. Description: G. haemolysans IgA1 protease Category: Unreleased Structures [[Category: Zhe...

New page

'''Unreleased structure'''

The entry 7uvk is ON HOLD

Authors: Eisenmesser, E.Z., Zheng, H.

Description: G. haemolysans IgA1 protease
[[Category: Unreleased Structures]]
[[Category: Zheng, H]]
[[Category: Eisenmesser, E.Z]]

7uvk - Revision history

OCA at 10:06, 24 November 2022

OCA at 03:50, 15 June 2022

OCA: Protected "7uvk" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7uvk is ON HOLD Authors: Eisenmesser, E.Z., Zheng, H. Description: G. haemolysans IgA1 protease Category: Unreleased Structures [[Category: Zhe...