OCA at 10:06, 24 November 2022

OCA — Thu, 24 Nov 2022 10:06:57 GMT

←Older revision		Revision as of 10:06, 24 November 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7uvl is ~~ON HOLD until Paper~~ Publication	+	==IgA1 Protease with IgA1 substrate==
		+	<StructureSection load='7uvl' size='340' side='right'caption='[[7uvl]], [[Resolution\|resolution]] 3.56Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7uvl]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Gemella_haemolysans Gemella haemolysans] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UVL FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uvl OCA], [https://pdbe.org/7uvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uvl RCSB], [https://www.ebi.ac.uk/pdbsum/7uvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uvl ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C5NYF3_9BACL C5NYF3_9BACL]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mucosal adaptive immune response is dependent on the production of IgA antibodies and particularly IgA1, yet opportunistic bacteria have evolved mechanisms to specifically block this response by producing IgA1 proteases (IgA1Ps). Our lab was the first to describe the structures of a metal-dependent IgA1P (metallo-IgA1P) produced from Gram-positive Streptococcus pneumoniae both in the absence and presence of its IgA1 substrate through cryo-EM single particle reconstructions. This prior study revealed an active-site gating mechanism reliant on substrate-induced conformational changes to the enzyme that begged the question of whether such a mechanism is conserved among the wider Gram-positive metallo-IgA1P subfamily of virulence factors. Here, we used cryo-EM to characterize the metallo-IgA1P of a more distantly related family member from Gemella haemolysans, an emerging opportunistic pathogen implicated in meningitis, endocarditis, and more recently bacteremia in the elderly. While the substrate-free structures of these two metallo-IgA1Ps exhibit differences in the relative starting positions of the domain responsible for gating substrate, the enzymes have similar domain orientations when bound to IgA1. Together with biochemical studies that indicate these metallo-IgA1Ps have similar binding affinities and activities, these data indicate that metallo-IgA1P binding requires the specific IgA1 substrate to open the enzymes for access to their active site and thus, largely conform to an "induced fit" model.

-	~~Authors: Eisenmesser, Z~~.E., ~~Zheng~~, H.	+	A substrate-induced gating mechanism is conserved among Gram-positive IgA1 metalloproteases.,Redzic JS, Rahkola J, Tran N, Holyoak T, Lee E, Martin-Galiano AJ, Meyer N, Zheng H, Eisenmesser E Commun Biol. 2022 Nov 7;5(1):1190. doi: 10.1038/s42003-022-04173-3. PMID:36336763<ref>PMID:36336763</ref>

-	~~Description~~: ~~IgA1 Protease with IgA1 substrate~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Zheng, H~~]]	+	<div class="pdbe-citations 7uvl" style="background-color:#fffaf0;"></div>
-	[[Category: Eisenmesser~~, Z.E~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gemella haemolysans]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Eisenmesser ZE]]
		+	[[Category: Zheng H]]

OCA at 16:29, 19 October 2022

OCA — Wed, 19 Oct 2022 16:29:17 GMT

←Older revision		Revision as of 16:29, 19 October 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uvl is ON HOLD	+	The entry 7uvl is ON HOLD until Paper Publication

	Authors: Eisenmesser, Z.E., Zheng, H.		Authors: Eisenmesser, Z.E., Zheng, H.

OCA at 15:17, 7 September 2022

OCA — Wed, 07 Sep 2022 15:17:27 GMT

←Older revision		Revision as of 15:17, 7 September 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uvl is ON HOLD ~~until Paper Publication~~	+	The entry 7uvl is ON HOLD

	Authors: Eisenmesser, Z.E., Zheng, H.		Authors: Eisenmesser, Z.E., Zheng, H.

OCA at 03:50, 15 June 2022

OCA — Wed, 15 Jun 2022 03:50:53 GMT

←Older revision		Revision as of 03:50, 15 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uvl is ON HOLD	+	The entry 7uvl is ON HOLD until Paper Publication

	Authors: Eisenmesser, Z.E., Zheng, H.		Authors: Eisenmesser, Z.E., Zheng, H.

OCA: Protected "7uvl" [edit=sysop:move=sysop]

OCA — Thu, 12 May 2022 04:48:27 GMT

Protected "7uvl" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:48, 12 May 2022

OCA: New page: '''Unreleased structure''' The entry 7uvl is ON HOLD Authors: Eisenmesser, Z.E., Zheng, H. Description: IgA1 Protease with IgA1 substrate Category: Unreleased Structures [[Category...

OCA — Thu, 12 May 2022 04:48:26 GMT

New page: '''Unreleased structure''' The entry 7uvl is ON HOLD Authors: Eisenmesser, Z.E., Zheng, H. Description: IgA1 Protease with IgA1 substrate Category: Unreleased Structures [[Category...

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