OCA at 10:07, 24 November 2022

OCA — Thu, 24 Nov 2022 10:07:00 GMT

←Older revision		Revision as of 10:07, 24 November 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7uw5 is ~~ON HOLD~~	+	==EcMscK G924S mutant in a closed conformation==
		+	<StructureSection load='7uw5' size='340' side='right'caption='[[7uw5]], [[Resolution\|resolution]] 3.84Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7uw5]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UW5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UW5 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uw5 OCA], [https://pdbe.org/7uw5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uw5 RCSB], [https://www.ebi.ac.uk/pdbsum/7uw5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uw5 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MSCK_ECOLI MSCK_ECOLI] Mechanosensitive channel that opens in response to membrane tension and specific ionic conditions. Requires high concentrations of external K(+), NH(4)(+), Rb(+) or Cs(+) to gate. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Forms an ion channel of 1.0 nanosiemens conductance. The channel can remain active for between 30 seconds and over 3 minutes; it does not desensitize upon extended pressure. Its activity is masked in wild-type cells by the MscS channel.<ref>PMID:10202137</ref> <ref>PMID:11985727</ref> <ref>PMID:12374733</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mechanosensitive channels of small conductance, found in many living organisms, open under elevated membrane tension and thus play crucial roles in biological response to mechanical stress. Amongst these channels, MscK is unique in that its activation also requires external potassium ions. To better understand this dual gating mechanism by force and ligand, we elucidate distinct structures of MscK along the gating cycle using cryo-electron microscopy. The heptameric channel comprises three layers: a cytoplasmic domain, a periplasmic gating ring, and a markedly curved transmembrane domain that flattens and expands upon channel opening, which is accompanied by dilation of the periplasmic ring. Furthermore, our results support a potentially unifying mechanotransduction mechanism in ion channels depicted as flattening and expansion of the transmembrane domain.

-	~~Authors~~:	+	Structural basis for mechanotransduction in a potassium-dependent mechanosensitive ion channel.,Mount J, Maksaev G, Summers BT, Fitzpatrick JAJ, Yuan P Nat Commun. 2022 Nov 12;13(1):6904. doi: 10.1038/s41467-022-34737-0. PMID:36371466<ref>PMID:36371466</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7uw5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli K-12]]
		+	[[Category: Large Structures]]
		+	[[Category: Mount JW]]
		+	[[Category: Yuan P]]

OCA at 04:24, 28 September 2022

OCA — Wed, 28 Sep 2022 04:24:08 GMT

←Older revision		Revision as of 04:24, 28 September 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uw5 is ON HOLD ~~until Paper Publication~~	+	The entry 7uw5 is ON HOLD

	Authors:		Authors:

OCA at 09:02, 18 May 2022

OCA — Wed, 18 May 2022 09:02:04 GMT

←Older revision		Revision as of 09:02, 18 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uw5 is ON HOLD	+	The entry 7uw5 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7uw5" [edit=sysop:move=sysop]

OCA — Thu, 12 May 2022 04:49:26 GMT

Protected "7uw5" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:49, 12 May 2022

OCA: New page: '''Unreleased structure''' The entry 7uw5 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 12 May 2022 04:49:25 GMT

New page: '''Unreleased structure''' The entry 7uw5 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7uw5 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7uw5 - Revision history

OCA at 10:07, 24 November 2022

OCA at 04:24, 28 September 2022

OCA at 09:02, 18 May 2022

OCA: Protected "7uw5" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7uw5 is ON HOLD Authors: Description: Category: Unreleased Structures