7v6b - Revision history

OCA at 04:48, 8 September 2022

OCA — Thu, 08 Sep 2022 04:48:11 GMT

←Older revision		Revision as of 04:48, 8 September 2022
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-	====	+	==Structure of the Dicer-2-R2D2 heterodimer==
-	<StructureSection load='7v6b' size='340' side='right'caption='[[7v6b]]' scene=''>	+	<StructureSection load='7v6b' size='340' side='right'caption='[[7v6b]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7v6b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V6B FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v6b OCA], [https://pdbe.org/7v6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v6b RCSB], [https://www.ebi.ac.uk/pdbsum/7v6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v6b ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v6b OCA], [https://pdbe.org/7v6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v6b RCSB], [https://www.ebi.ac.uk/pdbsum/7v6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v6b ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A1ZAW0_DROME A1ZAW0_DROME]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In flies, Argonaute2 (Ago2) and small interfering RNA (siRNA) form an RNA-induced silencing complex to repress viral transcripts(1). The RNase III enzyme Dicer-2 associates with its partner protein R2D2 and cleaves long double-stranded RNAs to produce 21-nucleotide siRNA duplexes, which are then loaded into Ago2 in a defined orientation(2-5). Here we report cryo-electron microscopy structures of the Dicer-2-R2D2 and Dicer-2-R2D2-siRNA complexes. R2D2 interacts with the helicase domain and the central linker of Dicer-2 to inhibit the promiscuous processing of microRNA precursors by Dicer-2. Notably, our structure represents the strand-selection state in the siRNA-loading process, and reveals that R2D2 asymmetrically recognizes the end of the siRNA duplex with the higher base-pairing stability, and the other end is exposed to the solvent and is accessible by Ago2. Our findings explain how R2D2 senses the thermodynamic asymmetry of the siRNA and facilitates the siRNA loading into Ago2 in a defined orientation, thereby determining which strand of the siRNA duplex is used by Ago2 as the guide strand for target silencing.
		+
		+	Structure of the Dicer-2-R2D2 heterodimer bound to a small RNA duplex.,Yamaguchi S, Naganuma M, Nishizawa T, Kusakizako T, Tomari Y, Nishimasu H, Nureki O Nature. 2022 Jul;607(7918):393-398. doi: 10.1038/s41586-022-04790-2. Epub 2022, Jun 29. PMID:35768503<ref>PMID:35768503</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7v6b" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Hirano H]]
		+	[[Category: Kusakizako T]]
		+	[[Category: Nishimasu H]]
		+	[[Category: Nishizawa T]]
		+	[[Category: Nureki O]]
		+	[[Category: Tomita A]]
		+	[[Category: Yamaguchi S]]
		+	[[Category: Yamashita K]]

OCA at 11:21, 23 March 2022

OCA — Wed, 23 Mar 2022 11:21:46 GMT

←Older revision		Revision as of 11:21, 23 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7v6b ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7v6b' size='340' side='right'caption='[[7v6b]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v6b OCA], [https://pdbe.org/7v6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v6b RCSB], [https://www.ebi.ac.uk/pdbsum/7v6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v6b ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:23, 15 September 2021

OCA — Wed, 15 Sep 2021 08:23:20 GMT

←Older revision		Revision as of 08:23, 15 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7v6b is ON HOLD	+	The entry 7v6b is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7v6b" [edit=sysop:move=sysop]

OCA — Wed, 25 Aug 2021 05:25:54 GMT

Protected "7v6b" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:25, 25 August 2021

OCA: New page: '''Unreleased structure''' The entry 7v6b is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 Aug 2021 05:25:54 GMT

New page: '''Unreleased structure''' The entry 7v6b is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7v6b is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]