7vbq - Revision history

OCA at 17:22, 29 November 2023

OCA — Wed, 29 Nov 2023 17:22:42 GMT

←Older revision		Revision as of 17:22, 29 November 2023
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-	====	+	==Heterodimer structure of Fe(II)/(alpha)ketoglutarate-dependent dioxygenase TlxIJ==
-	<StructureSection load='7vbq' size='340' side='right'caption='[[7vbq]]' scene=''>	+	<StructureSection load='7vbq' size='340' side='right'caption='[[7vbq]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vbq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Talaromyces_purpureogenus Talaromyces purpureogenus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VBQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vbq OCA], [https://pdbe.org/7vbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vbq RCSB], [https://www.ebi.ac.uk/pdbsum/7vbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vbq ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=OGA:N-OXALYLGLYCINE'>OGA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vbq OCA], [https://pdbe.org/7vbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vbq RCSB], [https://www.ebi.ac.uk/pdbsum/7vbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vbq ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Talaromyolides (1-6) are a group of unusual 6/6/6/6/6/6 hexacyclic meroterpenoids with (3R)-6-hydroxymellein and 4,5-seco-drimane substructures, isolated from the marine fungus Talaromyces purpureogenus. We have identified the biosynthetic gene cluster tlxA-J by heterologous expression in Aspergillus, in vitro enzyme assays, and CRISPR-Cas9-based gene inactivation. Remarkably, the heterodimer of non-heme iron (NHI) enzymes, TlxJ-TlxI, catalyzes three steps of oxidation including a key reaction, hydroxylation at C-5 and C-9 of 12, the intermediate with 3-ketohydroxydrimane scaffold, to facilitate a retro-aldol reaction, leading to the construction of the 4,5-secodrimane skeleton and characteristic ketal scaffold of 1-6. The products of TlxJ-TlxI, 1 and 4, were further hydroxylated at C-4'beta by another NHI heterodimer, TlxA-TlxC, and acetylated by TlxB to yield the final products, 3 and 6. The X-ray structural analysis coupled with site-directed mutagenesis provided insights into the heterodimer TlxJ-TlxI formation and its catalysis. This is the first report to show that two NHI proteins form a heterodimer for catalysis and utilizes a novel methodology to create functional oxygenase structures in secondary metabolite biosynthesis.
		+
		+	Heterodimeric Non-heme Iron Enzymes in Fungal Meroterpenoid Biosynthesis.,Li X, Awakawa T, Mori T, Ling M, Hu D, Wu B, Abe I J Am Chem Soc. 2021 Dec 22;143(50):21425-21432. doi: 10.1021/jacs.1c11548. Epub, 2021 Dec 9. PMID:34881885<ref>PMID:34881885</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vbq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Talaromyces purpureogenus]]
		+	[[Category: Abe I]]
		+	[[Category: Awakawa T]]
		+	[[Category: Li X]]
		+	[[Category: Mori T]]

OCA at 02:57, 21 April 2022

OCA — Thu, 21 Apr 2022 02:57:56 GMT

←Older revision		Revision as of 02:57, 21 April 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vbq ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7vbq' size='340' side='right'caption='[[7vbq]]' scene=''>
-	~~Authors~~: ~~Li, X~~.~~, Awakawa, T~~.~~, Mori, T~~.~~, Abe, I~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Heterodimer~~ structure ~~of Fe(II)~~/~~(alpha)ketoglutarate-dependent dioxygenase TlxIJ~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vbq OCA], [https://pdbe.org/7vbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vbq RCSB], [https://www.ebi.ac.uk/pdbsum/7vbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vbq ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures]~~]	+	</table>
-	[[~~Category~~: ~~Mori~~, T]]	+	__TOC__
-	[~~[Category~~: ~~Awakawa~~, T]]	+	</StructureSection>
-	[[Category: ~~Li, X~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Abe, I~~]]	+	[[Category: Z-disk]]

OCA at 08:25, 15 September 2021

OCA — Wed, 15 Sep 2021 08:25:25 GMT

←Older revision		Revision as of 08:25, 15 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vbq is ON HOLD	+	The entry 7vbq is ON HOLD until Paper Publication

	Authors: Li, X., Awakawa, T., Mori, T., Abe, I.		Authors: Li, X., Awakawa, T., Mori, T., Abe, I.

OCA: Protected "7vbq" [edit=sysop:move=sysop]

OCA — Wed, 08 Sep 2021 08:03:38 GMT

Protected "7vbq" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:03, 8 September 2021

OCA: New page: '''Unreleased structure''' The entry 7vbq is ON HOLD Authors: Li, X., Awakawa, T., Mori, T., Abe, I. Description: Heterodimer structure of Fe(II)/(alpha)ketoglutarate-dependent dioxyge...

OCA — Wed, 08 Sep 2021 08:03:37 GMT

New page: '''Unreleased structure''' The entry 7vbq is ON HOLD Authors: Li, X., Awakawa, T., Mori, T., Abe, I. Description: Heterodimer structure of Fe(II)/(alpha)ketoglutarate-dependent dioxyge...

New page

'''Unreleased structure'''

The entry 7vbq is ON HOLD

Authors: Li, X., Awakawa, T., Mori, T., Abe, I.

Description: Heterodimer structure of Fe(II)/(alpha)ketoglutarate-dependent dioxygenase TlxIJ
[[Category: Unreleased Structures]]
[[Category: Mori, T]]
[[Category: Awakawa, T]]
[[Category: Li, X]]
[[Category: Abe, I]]