7vco - Revision history

OCA at 17:23, 29 November 2023

OCA — Wed, 29 Nov 2023 17:23:07 GMT

←Older revision		Revision as of 17:23, 29 November 2023
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	<StructureSection load='7vco' size='340' side='right'caption='[[7vco]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='7vco' size='340' side='right'caption='[[7vco]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7vco]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VCO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vco]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Frischella_perrara Frischella perrara]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VCO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~, ~~<scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Beta-fructofuranosidase Beta-fructofuranosidase]~~, ~~with EC number [https~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.26 3.2.1.26]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vco OCA], [https://pdbe.org/7vco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vco RCSB], [https://www.ebi.ac.uk/pdbsum/7vco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vco ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vco OCA], [https://pdbe.org/7vco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vco RCSB], [https://www.ebi.ac.uk/pdbsum/7vco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vco ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A0A7S0J1_FRIPE A0A0A7S0J1_FRIPE]] Enables the bacterium to metabolize sucrose as a sole carbon source.[RuleBase:RU365015]	+	[https://www.uniprot.org/uniprot/A0A0A7S0J1_FRIPE A0A0A7S0J1_FRIPE] Enables the bacterium to metabolize sucrose as a sole carbon source.[RuleBase:RU365015]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Beta-fructofuranosidase~~]]	+	[[Category: Frischella perrara]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Tonozuka, T]]	+	[[Category: Tonozuka T]]
-	~~[[Category: Fructooligosaccharide]]~~	+
-	~~[[Category: Gh32]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 12:02, 23 March 2022

OCA — Wed, 23 Mar 2022 12:02:13 GMT

←Older revision		Revision as of 12:02, 23 March 2022
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/A0A0A7S0J1_FRIPE A0A0A7S0J1_FRIPE]] Enables the bacterium to metabolize sucrose as a sole carbon source.[RuleBase:RU365015]		[[https://www.uniprot.org/uniprot/A0A0A7S0J1_FRIPE A0A0A7S0J1_FRIPE]] Enables the bacterium to metabolize sucrose as a sole carbon source.[RuleBase:RU365015]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fructooligosaccharide is a mixture of mostly the trisaccharide 1-kestose (GF2), tetrasaccharide nystose (GF3), and fructosyl nystose (GF4). Enzymes that hydrolyze GF3 may be useful for preparing GF2 from the fructooligosaccharide mixture. A beta-fructofuranosidase belonging to glycoside hydrolase family 32 (GH32) from the honeybee gut bacterium Frischella perrara (FperFFase) was expressed in Escherichia coli and purified. The time course of the hydrolysis of 60 mM sucrose, GF2, and GF3 by FperFFase was analyzed, showing that the hydrolytic activity of FperFFase for trisaccharide GF2 was lower than those for disaccharide sucrose and tetrasaccharide GF3. The crystal structure of FperFFase and its structure in complex with fructose were determined. FperFFase was found to be structurally homologous to bifidobacterial beta-fructofuranosidases even though bifidobacterial enzymes preferably hydrolyze GF2 and the amino acid residues interacting with fructose at subsite - 1 are mostly conserved between them. A proline residue was inserted between Asp298 and Ser299 using site-directed mutagenesis, and the activity of the variant 298P299 was measured. The ratio of activities for 60 mM GF2/GF3 by wild-type FperFFase was 35.5%, while that of 298P299 was 23.6%, indicating that the structure of the loop comprising Trp297-Asp298-Ser299 correlated with the substrate preference of FperFFase. The crystal structure also shows that a loop consisting of residues 117-127 is likely to contribute to the substrate binding of FperFFase. The results obtained herein suggest that FperFFase is potentially useful for the manufacture of GF2. KEY POINTS: * Frischella beta-fructofuranosidase hydrolyzed nystose more efficiently than 1-kestose. * Trp297-Asp298-Ser299 was shown to be correlated with the substrate preference. * Loop consisting of residues 117-127 appears to contribute to the substrate binding.
		+
		+	Enzymatic and structural characterization of beta-fructofuranosidase from the honeybee gut bacterium Frischella perrara.,Kubota A, Kawai R, Li D, Kozono T, Sasaki N, Nishikawa A, Fujii T, Tochio T, Tonozuka T Appl Microbiol Biotechnol. 2022 Mar 10. pii: 10.1007/s00253-022-11863-9. doi:, 10.1007/s00253-022-11863-9. PMID:35267055<ref>PMID:35267055</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vco" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:25, 16 March 2022

OCA — Wed, 16 Mar 2022 07:25:47 GMT

←Older revision		Revision as of 07:25, 16 March 2022
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	==Frischella perrara beta-fructofuranosidase==		==Frischella perrara beta-fructofuranosidase==
-	<StructureSection load='7vco' size='340' side='right'caption='[[7vco]]' scene=''>	+	<StructureSection load='7vco' size='340' side='right'caption='[[7vco]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VCO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vco]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VCO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vco OCA], [https://pdbe.org/7vco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vco RCSB], [https://www.ebi.ac.uk/pdbsum/7vco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vco ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-fructofuranosidase Beta-fructofuranosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.26 3.2.1.26] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vco OCA], [https://pdbe.org/7vco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vco RCSB], [https://www.ebi.ac.uk/pdbsum/7vco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vco ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A0A7S0J1_FRIPE A0A0A7S0J1_FRIPE]] Enables the bacterium to metabolize sucrose as a sole carbon source.[RuleBase:RU365015]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Beta-fructofuranosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Tonozuka T]]	+	[[Category: Tonozuka, T]]
		+	[[Category: Fructooligosaccharide]]
		+	[[Category: Gh32]]
		+	[[Category: Hydrolase]]

OCA at 06:08, 9 March 2022

OCA — Wed, 09 Mar 2022 06:08:29 GMT

←Older revision		Revision as of 06:08, 9 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vco ~~is ON HOLD until Sep 03 2023~~	+	==Frischella perrara beta-fructofuranosidase==
-		+	<StructureSection load='7vco' size='340' side='right'caption='[[7vco]]' scene=''>
-	~~Authors~~: ~~Tonozuka, T~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VCO FirstGlance]. <br>
-	~~Description~~: ~~Frischella perrara beta~~-~~fructofuranosidase~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vco OCA], [https://pdbe.org/7vco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vco RCSB], [https://www.ebi.ac.uk/pdbsum/7vco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vco ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Tonozuka, T]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Tonozuka T]]

OCA at 07:52, 29 September 2021

OCA — Wed, 29 Sep 2021 07:52:32 GMT

←Older revision		Revision as of 07:52, 29 September 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vco is ON HOLD	+	The entry 7vco is ON HOLD until Sep 03 2023

	Authors: Tonozuka, T.		Authors: Tonozuka, T.

OCA: Protected "7vco" [edit=sysop:move=sysop]

OCA — Wed, 08 Sep 2021 08:05:18 GMT

Protected "7vco" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:05, 8 September 2021

OCA: New page: '''Unreleased structure''' The entry 7vco is ON HOLD Authors: Tonozuka, T. Description: Frischella perrara beta-fructofuranosidase Category: Unreleased Structures [[Category: Tonoz...

OCA — Wed, 08 Sep 2021 08:05:17 GMT

New page: '''Unreleased structure''' The entry 7vco is ON HOLD Authors: Tonozuka, T. Description: Frischella perrara beta-fructofuranosidase Category: Unreleased Structures [[Category: Tonoz...

New page

'''Unreleased structure'''

The entry 7vco is ON HOLD

Authors: Tonozuka, T.

Description: Frischella perrara beta-fructofuranosidase
[[Category: Unreleased Structures]]
[[Category: Tonozuka, T]]