7veh - Revision history

OCA at 19:42, 29 May 2024

OCA — Wed, 29 May 2024 19:42:46 GMT

←Older revision		Revision as of 19:42, 29 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7veh]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Moraxella_catarrhalis Moraxella catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VEH FirstGlance]. <br>		<table><tr><td colspan='2'>[[7veh]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Moraxella_catarrhalis Moraxella catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VEH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7veh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7veh OCA], [https://pdbe.org/7veh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7veh RCSB], [https://www.ebi.ac.uk/pdbsum/7veh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7veh ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.95Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7veh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7veh OCA], [https://pdbe.org/7veh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7veh RCSB], [https://www.ebi.ac.uk/pdbsum/7veh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7veh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A3A9QXE8_MORCA A0A3A9QXE8_MORCA]]	+	[https://www.uniprot.org/uniprot/A0A3A9QXE8_MORCA A0A3A9QXE8_MORCA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins provide prokaryotes with nucleic acid-based adaptive immunity against infections of mobile genetic elements, including phages. To counteract this immune process, phages have evolved various anti-CRISPR (Acr) proteins which deactivate CRISPR-Cas-based immunity. However, the mechanisms of many of these Acr-mediated inhibitions are not clear. Here, we report the crystal structure of AcrIF13 and explore its inhibition mechanism. The structure of AcrIF13 is unique and displays a negatively charged surface. Additionally, biochemical studies identified that AcrIF13 interacts with the type I-F CRISPR-Cas surveillance complex (Csy complex) to block target DNA recognition and that the Cas5f-8f tail and Cas7.6f subunit of the Csy complex are specific binding targets of AcrIF13. Further mutational studies demonstrated that several negatively charged residues of AcrIF13 and positively charged residues of Cas8f and Cas7f of the Csy complex are involved in AcrIF13-Csy binding. Together, our findings provide mechanistic insights into the inhibition mechanism of AcrIF13 and further suggest the prevalence of the function of Acr proteins as DNA mimics.
		+
		+	Mechanistic insights into the inhibition of the CRISPR-Cas surveillance complex by anti-CRISPR protein AcrIF13.,Wang H, Gao T, Zhou Y, Ren J, Guo J, Zeng J, Xiao Y, Zhang Y, Feng Y J Biol Chem. 2022 Mar;298(3):101636. doi: 10.1016/j.jbc.2022.101636. Epub 2022 , Jan 25. PMID:35085557<ref>PMID:35085557</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7veh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:47, 8 September 2022

OCA — Thu, 08 Sep 2022 06:47:53 GMT

←Older revision		Revision as of 06:47, 8 September 2022
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	==Type I-F Anti-CRISPR protein AcrIF13==		==Type I-F Anti-CRISPR protein AcrIF13==
-	<StructureSection load='7veh' size='340' side='right'caption='[[7veh]]' scene=''>	+	<StructureSection load='7veh' size='340' side='right'caption='[[7veh]], [[Resolution\|resolution]] 2.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VEH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7veh]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Moraxella_catarrhalis Moraxella catarrhalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VEH FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7veh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7veh OCA], [https://pdbe.org/7veh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7veh RCSB], [https://www.ebi.ac.uk/pdbsum/7veh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7veh ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7veh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7veh OCA], [https://pdbe.org/7veh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7veh RCSB], [https://www.ebi.ac.uk/pdbsum/7veh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7veh ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A3A9QXE8_MORCA A0A3A9QXE8_MORCA]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Moraxella catarrhalis]]
	[[Category: Feng Y]]		[[Category: Feng Y]]
	[[Category: Gao T]]		[[Category: Gao T]]

OCA at 16:15, 6 July 2022

OCA — Wed, 06 Jul 2022 16:15:18 GMT

←Older revision		Revision as of 16:15, 6 July 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7veh ~~is ON HOLD until Paper Publication~~	+	==Type I-F Anti-CRISPR protein AcrIF13==
-		+	<StructureSection load='7veh' size='340' side='right'caption='[[7veh]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VEH FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7veh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7veh OCA], [https://pdbe.org/7veh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7veh RCSB], [https://www.ebi.ac.uk/pdbsum/7veh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7veh ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Feng Y]]
		+	[[Category: Gao T]]

OCA at 07:52, 29 September 2021

OCA — Wed, 29 Sep 2021 07:52:44 GMT

←Older revision		Revision as of 07:52, 29 September 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7veh is ON HOLD	+	The entry 7veh is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7veh" [edit=sysop:move=sysop]

OCA — Wed, 15 Sep 2021 08:28:54 GMT

Protected "7veh" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:28, 15 September 2021

OCA: New page: '''Unreleased structure''' The entry 7veh is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Sep 2021 08:28:53 GMT

New page: '''Unreleased structure''' The entry 7veh is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7veh is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]