OCA at 10:24, 13 April 2022

OCA — Wed, 13 Apr 2022 10:24:51 GMT

←Older revision		Revision as of 10:24, 13 April 2022
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	==Cryo-EM structure of Vaccinia virus scaffolding protein D13 trimer sextet==		==Cryo-EM structure of Vaccinia virus scaffolding protein D13 trimer sextet==
-	<StructureSection load='7vfh' size='340' side='right'caption='[[7vfh]]' scene=''>	+	<StructureSection load='7vfh' size='340' side='right'caption='[[7vfh]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vfh]] is a 18 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFH FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vfh OCA], [https://pdbe.org/7vfh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vfh RCSB], [https://www.ebi.ac.uk/pdbsum/7vfh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vfh ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vfh OCA], [https://pdbe.org/7vfh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vfh RCSB], [https://www.ebi.ac.uk/pdbsum/7vfh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vfh ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/D13_VACCW D13_VACCW]] Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). This association occurs concomitantly with viral membrane formation. Targeted by the drug rifampicin, which prevents the formation of this lattice, and hence virus morphogenesis. In the presence of rifampicin, irregularly shaped membranes that lack the honeycomb layer accumulate around areas of electron-dense viroplasm. This layer is lost from virions during maturation from IV to mature virion (MV), through the proteolysis of A17 N-terminus.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In Vaccinia virus (VACV), the prototype poxvirus, scaffold protein D13 forms a honeycomb-like lattice on the viral membrane that results in formation of the pleomorphic immature virion (IV). The structure of D13 is similar to those of major capsid proteins that readily form icosahedral capsids in nucleocytoplasmic large DNA viruses (NCLDVs). However, the detailed assembly mechanism of the nonicosahedral poxvirus scaffold has never been understood. Here we show the cryo-EM structures of the D13 trimer and scaffold intermediates produced in vitro. The structures reveal that the displacement of the short N-terminal alpha-helix is critical for initiation of D13 self-assembly. The continuous curvature of the IV is mediated by electrostatic interactions that induce torsion between trimers. The assembly mechanism explains the semiordered capsid-like arrangement of D13 that is distinct from icosahedral NCLDVs. Our structures explain how a single protein can self-assemble into different capsid morphologies and represent a local exception to the universal Caspar-Klug theory of quasi-equivalence.
		+
		+	Assembly mechanism of the pleomorphic immature poxvirus scaffold.,Hyun J, Matsunami H, Kim TG, Wolf M Nat Commun. 2022 Mar 31;13(1):1704. doi: 10.1038/s41467-022-29305-5. PMID:35361762<ref>PMID:35361762</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vfh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hyun J]]	+	[[Category: Hyun, J]]
-	[[Category: Kim TG]]	+	[[Category: Kim, T G]]
-	[[Category: Matsunami H]]	+	[[Category: Matsunami, H]]
-	[[Category: Wolf M]]	+	[[Category: Wolf, M]]
		+	[[Category: Capsid]]
		+	[[Category: Double-jelly-roll]]
		+	[[Category: Scaffold]]
		+	[[Category: Viral protein]]

OCA at 07:45, 23 February 2022

OCA — Wed, 23 Feb 2022 07:45:17 GMT

←Older revision		Revision as of 07:45, 23 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vfh is ~~ON HOLD until Paper Publication~~	+	==Cryo-EM structure of Vaccinia virus scaffolding protein D13 trimer sextet==
-		+	<StructureSection load='7vfh' size='340' side='right'caption='[[7vfh]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFH FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vfh OCA], [https://pdbe.org/7vfh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vfh RCSB], [https://www.ebi.ac.uk/pdbsum/7vfh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vfh ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hyun J]]
		+	[[Category: Kim TG]]
		+	[[Category: Matsunami H]]
		+	[[Category: Wolf M]]

OCA at 15:11, 20 October 2021

OCA — Wed, 20 Oct 2021 15:11:46 GMT

←Older revision		Revision as of 15:11, 20 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vfh is ON HOLD	+	The entry 7vfh is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vfh" [edit=sysop:move=sysop]

OCA — Wed, 22 Sep 2021 05:44:05 GMT

Protected "7vfh" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:44, 22 September 2021

OCA: New page: '''Unreleased structure''' The entry 7vfh is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Sep 2021 05:44:04 GMT

New page: '''Unreleased structure''' The entry 7vfh is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vfh is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7vfh - Revision history

OCA at 10:24, 13 April 2022

OCA at 07:45, 23 February 2022

OCA at 15:11, 20 October 2021

OCA: Protected "7vfh" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7vfh is ON HOLD Authors: Description: Category: Unreleased Structures