7vft - Revision history

OCA at 17:24, 29 November 2023

OCA — Wed, 29 Nov 2023 17:24:43 GMT

←Older revision		Revision as of 17:24, 29 November 2023
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	<StructureSection load='7vft' size='340' side='right'caption='[[7vft]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='7vft' size='340' side='right'caption='[[7vft]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7vft]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vft]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vft OCA], [https://pdbe.org/7vft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vft RCSB], [https://www.ebi.ac.uk/pdbsum/7vft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vft ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vft OCA], [https://pdbe.org/7vft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vft RCSB], [https://www.ebi.ac.uk/pdbsum/7vft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vft ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: An, Y]]	+	[[Category: An Y]]
-	[[Category: Chan~~, H Y.E~~]]	+	[[Category: Chan HYE]]
-	[[Category: Ngo~~, J C.K]]~~	+	[[Category: Ngo JCK]]
-	~~[[Category: Expanded cag rna]]~~	+
-	~~[[Category: Polyq disease]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Rna toxicity~~]]	+

OCA at 05:14, 10 August 2022

OCA — Wed, 10 Aug 2022 05:14:16 GMT

←Older revision		Revision as of 05:14, 10 August 2022
Line 1:		Line 1:

	==Crystal structure of rGGGC(CAG)5GUCC oligo==		==Crystal structure of rGGGC(CAG)5GUCC oligo==
-	<StructureSection load='7vft' size='340' side='right'caption='[[7vft]]' scene=''>	+	<StructureSection load='7vft' size='340' side='right'caption='[[7vft]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vft]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFT FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vft OCA], [https://pdbe.org/7vft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vft RCSB], [https://www.ebi.ac.uk/pdbsum/7vft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vft ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vft OCA], [https://pdbe.org/7vft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vft RCSB], [https://www.ebi.ac.uk/pdbsum/7vft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vft ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polyglutamine (polyQ) diseases are a type of inherited neurodegenerative disorders caused by cytosine-adenine-guanine (CAG) trinucleotide expansion within the coding region of the disease-associated genes. We previously demonstrated that a pathogenic interaction between expanded CAG RNA and the nucleolin (NCL) protein triggers the nucleolar stress and neuronal cell death in polyQ diseases. However, mechanisms behind the molecular interaction remain unknown. Here, we report a 1.45 A crystal structure of the r(CAG)5 oligo that comprises a full A'-form helical turn with widened grooves. Based on this structure, we simulated a model of r(CAG)5 RNA complexed with the RNA recognition motif 2 (RRM2) of NCL and identified NCL residues that are critical for its binding to CAG RNA. Combined with in vitro and in vivo site-directed mutagenesis studies, our model reveals that CAG RNA binds to NCL sites that are not important for other cellular functions like gene expression and rRNA synthesis regulation, indicating that toxic CAG RNA interferes with NCL functions by sequestering it. Accordingly, an NCL mutant that is aberrant in CAG RNA-binding could rescue RNA-induced cytotoxicity effectively. Taken together, our study provides new molecular insights into the pathogenic mechanism of polyQ diseases mediated by NCL-CAG RNA interaction.
		+
		+	Molecular insights into the interaction of CAG trinucleotide RNA repeats with nucleolin and its implication in polyglutamine diseases.,An Y, Chen ZS, Chan HYE, Ngo JCK Nucleic Acids Res. 2022 Jul 22;50(13):7655-7668. doi: 10.1093/nar/gkac532. PMID:35776134<ref>PMID:35776134</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vft" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: An Y]]	+	[[Category: An, Y]]
-	[[Category: Chan ~~HYE~~]]	+	[[Category: Chan, H Y.E]]
-	[[Category: Ngo ~~JCK~~]]	+	[[Category: Ngo, J C.K]]
		+	[[Category: Expanded cag rna]]
		+	[[Category: Polyq disease]]
		+	[[Category: Rna]]
		+	[[Category: Rna toxicity]]

OCA at 05:11, 15 June 2022

OCA — Wed, 15 Jun 2022 05:11:53 GMT

←Older revision		Revision as of 05:11, 15 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vft is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of rGGGC(CAG)5GUCC oligo==
-		+	<StructureSection load='7vft' size='340' side='right'caption='[[7vft]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFT FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vft OCA], [https://pdbe.org/7vft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vft RCSB], [https://www.ebi.ac.uk/pdbsum/7vft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vft ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: An Y]]
		+	[[Category: Chan HYE]]
		+	[[Category: Ngo JCK]]

OCA at 12:18, 17 November 2021

OCA — Wed, 17 Nov 2021 12:18:02 GMT

←Older revision		Revision as of 12:18, 17 November 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vft is ON HOLD	+	The entry 7vft is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vft" [edit=sysop:move=sysop]

OCA — Wed, 22 Sep 2021 05:44:39 GMT

Protected "7vft" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:44, 22 September 2021

OCA: New page: '''Unreleased structure''' The entry 7vft is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Sep 2021 05:44:38 GMT

New page: '''Unreleased structure''' The entry 7vft is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vft is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]