7vh6 - Revision history

OCA at 11:13, 16 February 2022

OCA — Wed, 16 Feb 2022 11:13:55 GMT

←Older revision		Revision as of 11:13, 16 February 2022
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	==Cryo-EM structure of the hexameric plasma membrane H+-ATPase in the active state (pH 6.0, BeF3-, conformation 1, C1 symmetry)==		==Cryo-EM structure of the hexameric plasma membrane H+-ATPase in the active state (pH 6.0, BeF3-, conformation 1, C1 symmetry)==
-	<StructureSection load='7vh6' size='340' side='right'caption='[[7vh6]]' scene=''>	+	<StructureSection load='7vh6' size='340' side='right'caption='[[7vh6]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VH6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vh6]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VH6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vh6 OCA], [https://pdbe.org/7vh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vh6 RCSB], [https://www.ebi.ac.uk/pdbsum/7vh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vh6 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=POV:(2S)-3-(HEXADECANOYLOXY)-2-[(9Z)-OCTADEC-9-ENOYLOXY]PROPYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>POV</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/P-type_H(+)-exporting_transporter P-type H(+)-exporting transporter], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.1.2.1 7.1.2.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vh6 OCA], [https://pdbe.org/7vh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vh6 RCSB], [https://www.ebi.ac.uk/pdbsum/7vh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vh6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/PMA1_YEAST PMA1_YEAST]] The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The S. cerevisiae plasma membrane H(+)-ATPase, Pma1, is a P3A-type ATPase and the primary protein component of the membrane compartment of Pma1 (MCP). Like other plasma membrane H(+)-ATPases, Pma1 assembles and functions as a hexamer, a property unique to this subfamily among the larger family of P-type ATPases. It has been unclear how Pma1 organizes the yeast membrane into MCP microdomains, or why it is that Pma1 needs to assemble into a hexamer to establish the membrane electrochemical proton gradient. Here we report a high-resolution cryo-EM study of native Pma1 hexamers embedded in endogenous lipids. Remarkably, we found that the Pma1 hexamer encircles a liquid-crystalline membrane domain composed of 57 ordered lipid molecules. The Pma1-encircled lipid patch structure likely serves as the building block of the MCP. At pH 7.4, the carboxyl-terminal regulatory alpha-helix binds to the phosphorylation domains of two neighboring Pma1 subunits, locking the hexamer in the autoinhibited state. The regulatory helix becomes disordered at lower pH, leading to activation of the Pma1 hexamer. The activation process is accompanied by a 6.7 A downward shift and a 40 degrees rotation of transmembrane helices 1 and 2 that line the proton translocation path. The conformational changes have enabled us to propose a detailed mechanism for ATP-hydrolysis-driven proton pumping across the plasma membrane. Our structures will facilitate the development of antifungal drugs that target this essential protein.
		+
		+	Structure and activation mechanism of the hexameric plasma membrane H(+)-ATPase.,Zhao P, Zhao C, Chen D, Yun C, Li H, Bai L Nat Commun. 2021 Nov 8;12(1):6439. doi: 10.1038/s41467-021-26782-y. PMID:34750373<ref>PMID:34750373</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vh6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bai L]]	+	[[Category: Bai, L]]
-	[[Category: Chen D]]	+	[[Category: Chen, D]]
-	[[Category: Li H]]	+	[[Category: Li, H]]
-	[[Category: Yun C]]	+	[[Category: Yun, C]]
-	[[Category: Zhao C]]	+	[[Category: Zhao, C]]
-	[[Category: Zhao P]]	+	[[Category: Zhao, P]]
		+	[[Category: Hexamer]]
		+	[[Category: P type atpase]]
		+	[[Category: Plasma membrane h+-atpase]]
		+	[[Category: Translocase]]

OCA at 12:39, 24 November 2021

OCA — Wed, 24 Nov 2021 12:39:36 GMT

←Older revision		Revision as of 12:39, 24 November 2021
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-	'''Unreleased structure'''

-	~~The entry 7vh6 is ON HOLD until Paper Publication~~	+	==Cryo-EM structure of the hexameric plasma membrane H+-ATPase in the active state (pH 6.0, BeF3-, conformation 1, C1 symmetry)==
-		+	<StructureSection load='7vh6' size='340' side='right'caption='[[7vh6]]' scene=''>
-	~~Authors: Zhao, P., Zhao, C., Chen, D., Yun, C., Li, H., Bai, L.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VH6 FirstGlance]. <br>
-	~~Description:~~ Cryo-EM structure of the hexameric plasma membrane H+-ATPase in the active state (pH 6.0, BeF3-, conformation 1, C1 symmetry)	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vh6 OCA], [https://pdbe.org/7vh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vh6 RCSB], [https://www.ebi.ac.uk/pdbsum/7vh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vh6 ProSAT]</span></td></tr>
-	[[~~Category: Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Li, H]~~]	+	__TOC__
-	[[~~Category~~: ~~Zhao~~, C]]	+	</StructureSection>
-	[[Category: ~~Zhao, P~~]]	+	[[Category: Large Structures]]
-	[[Category: Bai, L]]	+	[[Category: Bai L]]
-	[[Category: Chen, D]]	+	[[Category: Chen D]]
-	[[Category: Yun, C]]	+	[[Category: Li H]]
		+	[[Category: Yun C]]
		+	[[Category: Zhao C]]
		+	[[Category: Zhao P]]

OCA at 12:18, 17 November 2021

OCA — Wed, 17 Nov 2021 12:18:10 GMT

←Older revision		Revision as of 12:18, 17 November 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vh6 is ON HOLD	+	The entry 7vh6 is ON HOLD until Paper Publication

	Authors: Zhao, P., Zhao, C., Chen, D., Yun, C., Li, H., Bai, L.		Authors: Zhao, P., Zhao, C., Chen, D., Yun, C., Li, H., Bai, L.
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	Description: Cryo-EM structure of the hexameric plasma membrane H+-ATPase in the active state (pH 6.0, BeF3-, conformation 1, C1 symmetry)		Description: Cryo-EM structure of the hexameric plasma membrane H+-ATPase in the active state (pH 6.0, BeF3-, conformation 1, C1 symmetry)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Zhao, P]]
	[[Category: Li, H]]		[[Category: Li, H]]
	[[Category: Zhao, C]]		[[Category: Zhao, C]]
		+	[[Category: Zhao, P]]
	[[Category: Bai, L]]		[[Category: Bai, L]]
	[[Category: Chen, D]]		[[Category: Chen, D]]
	[[Category: Yun, C]]		[[Category: Yun, C]]

OCA: Protected "7vh6" [edit=sysop:move=sysop]

OCA — Wed, 29 Sep 2021 07:53:26 GMT

Protected "7vh6" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:53, 29 September 2021

OCA: New page: '''Unreleased structure''' The entry 7vh6 is ON HOLD Authors: Zhao, P., Zhao, C., Chen, D., Yun, C., Li, H., Bai, L. Description: Cryo-EM structure of the hexameric plasma membrane H+-...

OCA — Wed, 29 Sep 2021 07:53:25 GMT

New page: '''Unreleased structure''' The entry 7vh6 is ON HOLD Authors: Zhao, P., Zhao, C., Chen, D., Yun, C., Li, H., Bai, L. Description: Cryo-EM structure of the hexameric plasma membrane H+-...

New page

'''Unreleased structure'''

The entry 7vh6 is ON HOLD

Authors: Zhao, P., Zhao, C., Chen, D., Yun, C., Li, H., Bai, L.

Description: Cryo-EM structure of the hexameric plasma membrane H+-ATPase in the active state (pH 6.0, BeF3-, conformation 1, C1 symmetry)
[[Category: Unreleased Structures]]
[[Category: Zhao, P]]
[[Category: Li, H]]
[[Category: Zhao, C]]
[[Category: Bai, L]]
[[Category: Chen, D]]
[[Category: Yun, C]]