7vk9 - Revision history

OCA at 17:27, 29 November 2023

OCA — Wed, 29 Nov 2023 17:27:23 GMT

←Older revision		Revision as of 17:27, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7vk9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_M1 Streptococcus pyogenes serotype M1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VK9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7vk9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_M1 Streptococcus pyogenes serotype M1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VK9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vk9 OCA], [https://pdbe.org/7vk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vk9 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vk9 OCA], [https://pdbe.org/7vk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vk9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>		[https://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>
		+
		+	==See Also==
		+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:33, 19 October 2022

OCA — Wed, 19 Oct 2022 19:33:54 GMT

←Older revision		Revision as of 19:33, 19 October 2022
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	==Crystal structure of xCas9 P411T==		==Crystal structure of xCas9 P411T==
-	<StructureSection load='7vk9' size='340' side='right'caption='[[7vk9]]' scene=''>	+	<StructureSection load='7vk9' size='340' side='right'caption='[[7vk9]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VK9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vk9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_M1 Streptococcus pyogenes serotype M1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VK9 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vk9 OCA], [https://pdbe.org/7vk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vk9 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vk9 OCA], [https://pdbe.org/7vk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vk9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Streptococcus pyogenes serotype M1]]
	[[Category: Bao R]]		[[Category: Bao R]]
	[[Category: Liu HY]]		[[Category: Liu HY]]
	[[Category: Luo YZ]]		[[Category: Luo YZ]]
	[[Category: Song YJ]]		[[Category: Song YJ]]

OCA at 10:27, 6 April 2022

OCA — Wed, 06 Apr 2022 10:27:29 GMT

←Older revision		Revision as of 10:27, 6 April 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vk9 ~~is ON HOLD until Apr 05 2023~~	+	==Crystal structure of xCas9 P411T==
-		+	<StructureSection load='7vk9' size='340' side='right'caption='[[7vk9]]' scene=''>
-	~~Authors~~: ~~Bao~~, R., ~~Liu~~, H.Y., ~~Luo, Y~~.Z., ~~Song, Y~~.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VK9 FirstGlance]. <br>
-	~~Description: Crystal structure of SpCas9 P411T~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vk9 OCA], [https://pdbe.org/7vk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vk9 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Liu, H.Y~~]]	+	__TOC__
-	[[Category: ~~Song, Y.J~~]]	+	</StructureSection>
-	[[Category: Luo~~, Y.Z~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Bao, R~~]]	+	[[Category: Bao R]]
		+	[[Category: Liu HY]]
		+	[[Category: Luo YZ]]
		+	[[Category: Song YJ]]

OCA at 09:37, 30 March 2022

OCA — Wed, 30 Mar 2022 09:37:49 GMT

←Older revision		Revision as of 09:37, 30 March 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vk9 is ON HOLD until ~~Mar 29~~ 2023	+	The entry 7vk9 is ON HOLD until Apr 05 2023

	Authors: Bao, R., Liu, H.Y., Luo, Y.Z., Song, Y.J.		Authors: Bao, R., Liu, H.Y., Luo, Y.Z., Song, Y.J.

OCA at 12:23, 29 December 2021

OCA — Wed, 29 Dec 2021 12:23:18 GMT

←Older revision		Revision as of 12:23, 29 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vk9 is ON HOLD	+	The entry 7vk9 is ON HOLD until Mar 29 2023

	Authors: Bao, R., Liu, H.Y., Luo, Y.Z., Song, Y.J.		Authors: Bao, R., Liu, H.Y., Luo, Y.Z., Song, Y.J.
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	Description: Crystal structure of SpCas9 P411T		Description: Crystal structure of SpCas9 P411T
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Liu, H.Y]]
	[[Category: Song, Y.J]]		[[Category: Song, Y.J]]
	[[Category: Luo, Y.Z]]		[[Category: Luo, Y.Z]]
	[[Category: Bao, R]]		[[Category: Bao, R]]
-	[[Category: Liu, H.Y]]

OCA: Protected "7vk9" [edit=sysop:move=sysop]

OCA — Wed, 06 Oct 2021 04:25:31 GMT

Protected "7vk9" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:25, 6 October 2021

OCA: New page: '''Unreleased structure''' The entry 7vk9 is ON HOLD Authors: Bao, R., Liu, H.Y., Luo, Y.Z., Song, Y.J. Description: Crystal structure of SpCas9 P411T [[Category: Unreleased Structures...

OCA — Wed, 06 Oct 2021 04:25:30 GMT

New page: '''Unreleased structure''' The entry 7vk9 is ON HOLD Authors: Bao, R., Liu, H.Y., Luo, Y.Z., Song, Y.J. Description: Crystal structure of SpCas9 P411T [[Category: Unreleased Structures...

New page

'''Unreleased structure'''

The entry 7vk9 is ON HOLD

Authors: Bao, R., Liu, H.Y., Luo, Y.Z., Song, Y.J.

Description: Crystal structure of SpCas9 P411T
[[Category: Unreleased Structures]]
[[Category: Song, Y.J]]
[[Category: Luo, Y.Z]]
[[Category: Bao, R]]
[[Category: Liu, H.Y]]