OCA at 06:04, 26 October 2022

OCA — Wed, 26 Oct 2022 06:04:58 GMT

←Older revision		Revision as of 06:04, 26 October 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7vnj is ~~ON HOLD until Paper~~ Publication	+	==Complex structure of Clostridioides difficile enzymatic component (CDTa) and binding component (CDTb) pore with short stem==
		+	<StructureSection load='7vnj' size='340' side='right'caption='[[7vnj]], [[Resolution\|resolution]] 2.56Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7vnj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile Clostridioides difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VNJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VNJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vnj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vnj OCA], [https://pdbe.org/7vnj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vnj RCSB], [https://www.ebi.ac.uk/pdbsum/7vnj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vnj ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A8DS70_CLODI A8DS70_CLODI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Some bacteria express a binary toxin translocation system, consisting of an enzymatic subunit and translocation pore, that delivers enzymes into host cells through endocytosis. The most clinically important bacterium with such a system is Clostridioides difficile (formerly Clostridium). The CDTa and CDTb proteins from its system represent important therapeutic targets. CDTb has been proposed to be a di-heptamer, but its physiological heptameric structure has not yet been reported. Here, we report the cryo-EM structure of CDTa bound to the CDTb-pore, which reveals that CDTa binding induces partial unfolding and tilting of the first CDTa alpha-helix. In the CDTb-pore, an NSS-loop exists in 'in' and 'out' conformations, suggesting its involvement in substrate translocation. Finally, 3D variability analysis revealed CDTa movements from a folded to an unfolded state. These dynamic structural information provide insights into drug design against hypervirulent C. difficile strains.

-	~~Authors~~:	+	Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore from Clostridioides difficile.,Kawamoto A, Yamada T, Yoshida T, Sato Y, Kato T, Tsuge H Nat Commun. 2022 Oct 17;13(1):6119. doi: 10.1038/s41467-022-33888-4. PMID:36253419<ref>PMID:36253419</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7vnj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Clostridioides difficile]]
		+	[[Category: Large Structures]]
		+	[[Category: Kato T]]
		+	[[Category: Kawamoto A]]
		+	[[Category: Sato Y]]
		+	[[Category: Tsuge H]]
		+	[[Category: Yamada T]]
		+	[[Category: Yoshida T]]

OCA at 05:16, 31 August 2022

OCA — Wed, 31 Aug 2022 05:16:46 GMT

←Older revision		Revision as of 05:16, 31 August 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vnj is ON HOLD	+	The entry 7vnj is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 03:30, 25 August 2022

OCA — Thu, 25 Aug 2022 03:30:46 GMT

←Older revision		Revision as of 03:30, 25 August 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vnj is ON HOLD ~~until Paper Publication~~	+	The entry 7vnj is ON HOLD

	Authors:		Authors:

OCA at 09:37, 30 March 2022

OCA — Wed, 30 Mar 2022 09:37:52 GMT

←Older revision		Revision as of 09:37, 30 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vnj is ON HOLD	+	The entry 7vnj is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vnj" [edit=sysop:move=sysop]

OCA — Wed, 20 Oct 2021 15:16:35 GMT

Protected "7vnj" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:16, 20 October 2021

OCA: New page: '''Unreleased structure''' The entry 7vnj is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Oct 2021 15:16:34 GMT

New page: '''Unreleased structure''' The entry 7vnj is ON HOLD Authors: Description: Category: Unreleased Structures

New page