7vo0 - Revision history

OCA at 03:21, 8 September 2022

OCA — Thu, 08 Sep 2022 03:21:37 GMT

←Older revision		Revision as of 03:21, 8 September 2022
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	==Streptomyces coelicolor zinc uptake regulator complexed with zinc and DNA (trimer of dimers)==		==Streptomyces coelicolor zinc uptake regulator complexed with zinc and DNA (trimer of dimers)==
-	<StructureSection load='7vo0' size='340' side='right'caption='[[7vo0]]' scene=''>	+	<StructureSection load='7vo0' size='340' side='right'caption='[[7vo0]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VO0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vo0]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VO0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vo0 OCA], [https://pdbe.org/7vo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vo0 RCSB], [https://www.ebi.ac.uk/pdbsum/7vo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vo0 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vo0 OCA], [https://pdbe.org/7vo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vo0 RCSB], [https://www.ebi.ac.uk/pdbsum/7vo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vo0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/Q9L2H5_STRCO Q9L2H5_STRCO]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Streptomyces coelicolor (Sc) is a model organism of actinobacteria to study morphological differentiation and production of bioactive metabolites. Sc zinc uptake regulator (Zur) affects both processes by controlling zinc homeostasis. It activates transcription by binding to palindromic Zur-box sequences upstream of -35 elements. Here we deciphered the molecular mechanism by which ScZur interacts with promoter DNA and Sc RNA polymerase (RNAP) by cryo-EM structures and biochemical assays. The ScZur-DNA structures reveal a sequential and cooperative binding of three ScZur dimers surrounding a Zur-box spaced 8 nt upstream from a -35 element. The ScRNAPsigmaHrdB-Zur-DNA structures define protein-protein and protein-DNA interactions involved in the principal housekeeping sigmaHrdB-dependent transcription initiation from a noncanonical promoter with a -10 element lacking the critical adenine residue at position -11 and a TTGCCC -35 element deviating from the canonical TTGACA motif. ScZur interacts with the C-terminal domain of ScRNAP alpha subunit (alphaCTD) in a complex structure trapped in an active conformation. Key ScZur-alphaCTD interfacial residues accounting for ScZur-dependent transcription activation were confirmed by mutational studies. Together, our structural and biochemical results provide a comprehensive model for transcription activation of Zur family regulators.
		+
		+	Structural basis of Streptomyces transcription activation by zinc uptake regulator.,Yang X, Wang Y, Liu G, Deng Z, Lin S, Zheng J Nucleic Acids Res. 2022 Aug 12;50(14):8363-8376. doi: 10.1093/nar/gkac627. PMID:35871291<ref>PMID:35871291</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vo0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:12, 31 August 2022

OCA — Wed, 31 Aug 2022 11:12:27 GMT

←Older revision		Revision as of 11:12, 31 August 2022
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-	====	+	==Streptomyces coelicolor zinc uptake regulator complexed with zinc and DNA (trimer of dimers)==
	<StructureSection load='7vo0' size='340' side='right'caption='[[7vo0]]' scene=''>		<StructureSection load='7vo0' size='340' side='right'caption='[[7vo0]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VO0 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vo0 OCA], [https://pdbe.org/7vo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vo0 RCSB], [https://www.ebi.ac.uk/pdbsum/7vo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vo0 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vo0 OCA], [https://pdbe.org/7vo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vo0 RCSB], [https://www.ebi.ac.uk/pdbsum/7vo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vo0 ProSAT]</span></td></tr>
	</table>		</table>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Yang X]]
		+	[[Category: Zheng J]]

OCA at 04:54, 3 August 2022

OCA — Wed, 03 Aug 2022 04:54:25 GMT

←Older revision		Revision as of 04:54, 3 August 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7vo0 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7vo0' size='340' side='right'caption='[[7vo0]]' scene=''>
-	~~Authors~~: ~~Yang, X~~.~~, Zheng, J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Streptomyces coelicolor zinc uptake regulator complexed with zinc and DNA (trimer of dimers)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vo0 OCA], [https://pdbe.org/7vo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vo0 RCSB], [https://www.ebi.ac.uk/pdbsum/7vo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vo0 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Yang, X~~]]	+	__TOC__
-	[[Category: ~~Zheng, J~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 12:49, 27 October 2021

OCA — Wed, 27 Oct 2021 12:49:44 GMT

←Older revision		Revision as of 12:49, 27 October 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vo0 is ON HOLD	+	The entry 7vo0 is ON HOLD until Paper Publication

	Authors: Yang, X., Zheng, J.		Authors: Yang, X., Zheng, J.

OCA: Protected "7vo0" [edit=sysop:move=sysop]

OCA — Wed, 20 Oct 2021 15:17:23 GMT

Protected "7vo0" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:17, 20 October 2021

OCA: New page: '''Unreleased structure''' The entry 7vo0 is ON HOLD Authors: Yang, X., Zheng, J. Description: Streptomyces coelicolor zinc uptake regulator complexed with zinc and DNA (trimer of dime...

OCA — Wed, 20 Oct 2021 15:17:22 GMT

New page: '''Unreleased structure''' The entry 7vo0 is ON HOLD Authors: Yang, X., Zheng, J. Description: Streptomyces coelicolor zinc uptake regulator complexed with zinc and DNA (trimer of dime...

New page

'''Unreleased structure'''

The entry 7vo0 is ON HOLD

Authors: Yang, X., Zheng, J.

Description: Streptomyces coelicolor zinc uptake regulator complexed with zinc and DNA (trimer of dimers)
[[Category: Unreleased Structures]]
[[Category: Yang, X]]
[[Category: Zheng, J]]