7vq6 - Revision history

OCA at 17:30, 29 November 2023

OCA — Wed, 29 Nov 2023 17:30:14 GMT

←Older revision		Revision as of 17:30, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7vq6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gossypium_hirsutum Gossypium hirsutum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VQ6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7vq6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gossypium_hirsutum Gossypium hirsutum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VQ6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.39Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vq6 OCA], [https://pdbe.org/7vq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vq6 RCSB], [https://www.ebi.ac.uk/pdbsum/7vq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vq6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vq6 OCA], [https://pdbe.org/7vq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vq6 RCSB], [https://www.ebi.ac.uk/pdbsum/7vq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vq6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A1U8Q028_GOSHI A0A1U8Q028_GOSHI]] Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.[ARBA:ARBA00003610][RuleBase:RU361179]	+	[https://www.uniprot.org/uniprot/A0A1U8Q028_GOSHI A0A1U8Q028_GOSHI] Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.[ARBA:ARBA00003610][RuleBase:RU361179]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7vq6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7vq6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glyoxalase 3D structures\|Glyoxalase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:18, 8 September 2022

OCA — Thu, 08 Sep 2022 04:18:04 GMT

←Older revision		Revision as of 04:18, 8 September 2022
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	==Structure of a specialized glyoxalase from Gossypium hirsutum==		==Structure of a specialized glyoxalase from Gossypium hirsutum==
-	<StructureSection load='7vq6' size='340' side='right'caption='[[7vq6]]' scene=''>	+	<StructureSection load='7vq6' size='340' side='right'caption='[[7vq6]], [[Resolution\|resolution]] 1.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VQ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vq6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gossypium_hirsutum Gossypium hirsutum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VQ6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vq6 OCA], [https://pdbe.org/7vq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vq6 RCSB], [https://www.ebi.ac.uk/pdbsum/7vq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vq6 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vq6 OCA], [https://pdbe.org/7vq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vq6 RCSB], [https://www.ebi.ac.uk/pdbsum/7vq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vq6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A1U8Q028_GOSHI A0A1U8Q028_GOSHI]] Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.[ARBA:ARBA00003610][RuleBase:RU361179]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Deoxynivalenol (DON) and its acetylated derivatives such as 3-acetyldeoxynivalenol (3A-DON) and 15-acetyldeoxynivalenol (15A-DON) are notorious mycotoxins in Fusarium contaminated cereals, which pose a great threat to human and livestock health. The specialized glyoxalase I from Gossypium hirsutum (SPG) can lower the toxicity of 3A-DON by conducting isomerization to transfer C8 carbonyl to C7 and double bond from C9-C10 to C8-C9. Here we report that the substrate-flexible SPG can also recognize 15A-DON and DON, probably following the same isomerization mechanism as that for 3A-DON. The crystallographic, mutagenesis, and biochemical analyses revealed that SPG provides a hydrophobic pocket to accommodate the substrate and residue E167 might serve as the catalytic base. A variant SPG(Y62A) that was constructed based on structure-based protein engineering exhibited elevated catalytic activity towards DON, 3A-DON, and 15A-DON by >70%. Furthermore, variant SPG(Y62A) was successfully expressed in Pichia pastoris, whose catalytic activity was also compared to that produced in Escherichia coli. These results provide a blueprint for further protein engineering of SPG and reveal the potential applications of the enzyme in detoxifying DON, 3A-DON and 15A-DON.
		+
		+	Crystal structure and biochemical analysis of the specialized deoxynivalenol-detoxifying glyoxalase SPG from Gossypium hirsutum.,Hu Y, Li H, Min J, Yu Y, Liu W, Huang JW, Zhang L, Yang Y, Dai L, Chen CC, Guo RT Int J Biol Macromol. 2022 Mar 1;200:388-396. doi: 10.1016/j.ijbiomac.2022.01.055., Epub 2022 Jan 18. PMID:35051496<ref>PMID:35051496</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vq6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gossypium hirsutum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chen CC]]		[[Category: Chen CC]]

OCA at 18:06, 27 July 2022

OCA — Wed, 27 Jul 2022 18:06:14 GMT

←Older revision		Revision as of 18:06, 27 July 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vq6 is ~~ON HOLD until Paper Publication~~	+	==Structure of a specialized glyoxalase from Gossypium hirsutum==
-		+	<StructureSection load='7vq6' size='340' side='right'caption='[[7vq6]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VQ6 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vq6 OCA], [https://pdbe.org/7vq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vq6 RCSB], [https://www.ebi.ac.uk/pdbsum/7vq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vq6 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chen CC]]
		+	[[Category: Dai LH]]
		+	[[Category: Guo RT]]
		+	[[Category: Hu YM]]
		+	[[Category: Huang JW]]
		+	[[Category: Li H]]
		+	[[Category: Liu WD]]

OCA at 05:20, 10 November 2021

OCA — Wed, 10 Nov 2021 05:20:30 GMT

←Older revision		Revision as of 05:20, 10 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vq6 is ON HOLD	+	The entry 7vq6 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vq6" [edit=sysop:move=sysop]

OCA — Wed, 27 Oct 2021 12:52:14 GMT

Protected "7vq6" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:52, 27 October 2021

OCA: New page: '''Unreleased structure''' The entry 7vq6 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Oct 2021 12:52:12 GMT

New page: '''Unreleased structure''' The entry 7vq6 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vq6 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]