7vt8 - Revision history

OCA at 17:32, 29 November 2023

OCA — Wed, 29 Nov 2023 17:32:09 GMT

←Older revision		Revision as of 17:32, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7vt8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Meiothermus_taiwanensis_WR-220 Meiothermus taiwanensis WR-220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VT8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7vt8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Meiothermus_taiwanensis_WR-220 Meiothermus taiwanensis WR-220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VT8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.99Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vt8 OCA], [https://pdbe.org/7vt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7vt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vt8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vt8 OCA], [https://pdbe.org/7vt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7vt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vt8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A399DY85_9DEIN A0A399DY85_9DEIN]]	+	[https://www.uniprot.org/uniprot/A0A399DY85_9DEIN A0A399DY85_9DEIN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:43, 7 September 2022

OCA — Wed, 07 Sep 2022 18:43:31 GMT

←Older revision		Revision as of 18:43, 7 September 2022
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/A0A399DY85_9DEIN A0A399DY85_9DEIN]]		[[https://www.uniprot.org/uniprot/A0A399DY85_9DEIN A0A399DY85_9DEIN]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Most known cellulase-associated carbohydrate-binding modules (CBMs) are attached to the N- or C-terminus of the enzyme or are expressed separately and assembled into multi-enzyme complexes (for example to form cellulosomes), rather than being an insertion into the catalytic domain. Here, by solving the crystal structure, it is shown that MtGlu5 from Meiothermus taiwanensis WR-220, a GH5-family endo-beta-1,4-glucanase (EC 3.2.1.4), has a bipartite architecture consisting of a Cel5A-like catalytic domain with a (beta/alpha)8 TIM-barrel fold and an inserted CBM29-like noncatalytic domain with a beta-jelly-roll fold. Deletion of the CBM significantly reduced the catalytic efficiency of MtGlu5, as determined by isothermal titration calorimetry using inactive mutants of full-length and CBM-deleted MtGlu5 proteins. Conversely, insertion of the CBM from MtGlu5 into TmCel5A from Thermotoga maritima greatly enhanced the substrate affinity of TmCel5A. Bound sugars observed between two tryptophan side chains in the catalytic domains of active full-length and CBM-deleted MtGlu5 suggest an important stacking force. The synergistic action of the catalytic domain and CBM of MtGlu5 in binding to single-chain polysaccharides was visualized by substrate modeling, in which additional surface tryptophan residues were identified in a cross-domain groove. Subsequent site-specific mutagenesis results confirmed the pivotal role of several other tryptophan residues from both domains of MtGlu5 in substrate binding. These findings reveal a way to incorporate a CBM into the catalytic domain of an existing enzyme to make a robust cellulase.
		+
		+	Synergic action of an inserted carbohydrate-binding module in a glycoside hydrolase family 5 endoglucanase.,Ye TJ, Huang KF, Ko TP, Wu SH Acta Crystallogr D Struct Biol. 2022 May 1;78(Pt 5):633-646. doi:, 10.1107/S2059798322002601. Epub 2022 Apr 20. PMID:35503211<ref>PMID:35503211</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vt8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:39, 7 September 2022

OCA — Wed, 07 Sep 2022 16:39:09 GMT

←Older revision		Revision as of 16:39, 7 September 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vt8 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of MtGlu5 from Meiothermus taiwanensis WR-220==
-		+	<StructureSection load='7vt8' size='340' side='right'caption='[[7vt8]], [[Resolution\|resolution]] 2.99Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[7vt8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Meiothermus_taiwanensis_WR-220 Meiothermus taiwanensis WR-220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VT8 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vt8 OCA], [https://pdbe.org/7vt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7vt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vt8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A399DY85_9DEIN A0A399DY85_9DEIN]]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Meiothermus taiwanensis WR-220]]
		+	[[Category: Huang KF]]
		+	[[Category: Ko PT]]
		+	[[Category: Wu SH]]
		+	[[Category: Ye TJ]]

OCA at 12:19, 17 November 2021

OCA — Wed, 17 Nov 2021 12:19:15 GMT

←Older revision		Revision as of 12:19, 17 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vt8 is ON HOLD	+	The entry 7vt8 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vt8" [edit=sysop:move=sysop]

OCA — Wed, 03 Nov 2021 13:04:06 GMT

Protected "7vt8" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:04, 3 November 2021

OCA: New page: '''Unreleased structure''' The entry 7vt8 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Nov 2021 13:04:05 GMT

New page: '''Unreleased structure''' The entry 7vt8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vt8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]