7vt9 - Revision history

OCA at 17:32, 29 November 2023

OCA — Wed, 29 Nov 2023 17:32:12 GMT

←Older revision		Revision as of 17:32, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7vt9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VT9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7vt9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VT9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vt9 OCA], [https://pdbe.org/7vt9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vt9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vt9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vt9 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vt9 OCA], [https://pdbe.org/7vt9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vt9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vt9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vt9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 06:05, 26 October 2022

OCA — Wed, 26 Oct 2022 06:05:22 GMT

←Older revision		Revision as of 06:05, 26 October 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vt9 is ~~ON HOLD until Paper~~ Publication	+	==CRYSTAL STRUCTURE AT 3.4 ANGSTROMS RESOLUTION OF Maltodextrin glucosidase, MalZ, FROM Escherichia coli==
		+	<StructureSection load='7vt9' size='340' side='right'caption='[[7vt9]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7vt9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VT9 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vt9 OCA], [https://pdbe.org/7vt9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vt9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vt9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vt9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MALZ_ECOLI MALZ_ECOLI] May play a role in regulating the intracellular level of maltotriose. Cleaves glucose from the reducing end of maltotriose and longer maltodextrins with a chain length of up to 7 glucose units.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Maltodextrin glucosidase (MalZ) is a key enzyme in the maltose utilization pathway in Escherichia coli that liberates glucose from the reducing end of the short malto-oligosaccharides. Unlike other enzymes in the GH13_21 subfamily, the hydrolytic activity of MalZ is limited to maltodextrin rather than long starch substrates, forming various transglycosylation products in alpha-1,3, alpha-1,4 or alpha-1,6 linkages. The mechanism for the substrate binding and hydrolysis of this enzyme is not well understood yet. Here, we present the dimeric crystal structure of MalZ, with the N-domain generating a unique substrate binding groove. The N-domain bears CBM34 architecture and forms a part of the active site in the catalytic domain of the adjacent molecule. The groove found between the N-domain and catalytic domain from the adjacent molecule, shapes active sites suitable for short malto-oligosaccharides, but hinders long stretches of oligosaccharides. The conserved residue of E44 protrudes at subsite +2, elucidating the hydrolysis pattern of the substrate by the glucose unit from the reducing end. The structural analysis provides a molecular basis for the substrate specificity and the enzymatic property, and has potential industrial application for protein engineering.

-	~~Authors~~:	+	Dimeric architecture of maltodextrin glucosidase (MalZ) provides insights into the substrate recognition and hydrolysis mechanism.,Ahn WC, An Y, Song KM, Park KH, Lee SJ, Oh BH, Park JT, Woo EJ Biochem Biophys Res Commun. 2022 Jan 1;586:49-54. doi:, 10.1016/j.bbrc.2021.11.070. Epub 2021 Nov 20. PMID:34826700<ref>PMID:34826700</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7vt9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli K-12]]
		+	[[Category: Large Structures]]
		+	[[Category: Ahn W-C]]
		+	[[Category: Ahn Y]]
		+	[[Category: Woo E-J]]

OCA at 12:19, 17 November 2021

OCA — Wed, 17 Nov 2021 12:19:17 GMT

←Older revision		Revision as of 12:19, 17 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vt9 is ON HOLD	+	The entry 7vt9 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vt9" [edit=sysop:move=sysop]

OCA — Wed, 03 Nov 2021 13:04:09 GMT

Protected "7vt9" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:04, 3 November 2021

OCA: New page: '''Unreleased structure''' The entry 7vt9 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Nov 2021 13:04:08 GMT

New page: '''Unreleased structure''' The entry 7vt9 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vt9 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]