7vvz - Revision history

OCA at 06:27, 26 October 2022

OCA — Wed, 26 Oct 2022 06:27:28 GMT

←Older revision		Revision as of 06:27, 26 October 2022
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-	====	+	==NuA4 bound to the nucleosome==
-	<StructureSection load='7vvz' size='340' side='right'caption='[[7vvz]]' scene=''>	+	<StructureSection load='7vvz' size='340' side='right'caption='[[7vvz]], [[Resolution\|resolution]] 8.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vvz]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VVZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vvz OCA], [https://pdbe.org/7vvz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vvz RCSB], [https://www.ebi.ac.uk/pdbsum/7vvz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vvz ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CMC:CARBOXYMETHYL+COENZYME+*A'>CMC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vvz OCA], [https://pdbe.org/7vvz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vvz RCSB], [https://www.ebi.ac.uk/pdbsum/7vvz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vvz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/EAF6_YEAST EAF6_YEAST] Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Component of the NuA3 histone acetyltransferase complex, that acetylates Lys-14 of histone H3. Recruitment of NuA3 to nucleosomes requires methylated histone H3. In conjunction with the FACT complex, NuA3 may be involved in transcriptional regulation.<ref>PMID:17157260</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Deoxyribonucleic acid in eukaryotes wraps around the histone octamer to form nucleosomes(1), the fundamental unit of chromatin. The N termini of histone H4 interact with nearby nucleosomes and play an important role in the formation of high-order chromatin structure and heterochromatin silencing(2-4). NuA4 in yeast and its homologue Tip60 complex in mammalian cells are the key enzymes that catalyse H4 acetylation, which in turn regulates chromatin packaging and function in transcription activation and DNA repair(5-10). Here we report the cryo-electron microscopy structure of NuA4 from Saccharomyces cerevisiae bound to the nucleosome. NuA4 comprises two major modules: the catalytic histone acetyltransferase (HAT) module and the transcription activator-binding (TRA) module. The nucleosome is mainly bound by the HAT module and is positioned close to a polybasic surface of the TRA module, which is important for the optimal activity of NuA4. The nucleosomal linker DNA carrying the upstream activation sequence is oriented towards the conserved, transcription activator-binding surface of the Tra1 subunit, which suggests a potential mechanism of NuA4 to act as a transcription co-activator. The HAT module recognizes the disk face of the nucleosome through the H2A-H2B acidic patch and nucleosomal DNA, projecting the catalytic pocket of Esa1 to the N-terminal tail of H4 and supporting its function in selective acetylation of H4. Together, our findings illustrate how NuA4 is assembled and provide mechanistic insights into nucleosome recognition and transcription co-activation by a HAT.
		+
		+	Structure of the NuA4 acetyltransferase complex bound to the nucleosome.,Qu K, Chen K, Wang H, Li X, Chen Z Nature. 2022 Oct;610(7932):569-574. doi: 10.1038/s41586-022-05303-x. Epub 2022, Oct 5. PMID:36198799<ref>PMID:36198799</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vvz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z~~-disk~~]]	+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Saccharomyces cerevisiae S288C]]
		+	[[Category: Chen Z]]
		+	[[Category: Qu K]]

OCA at 05:01, 10 August 2022

OCA — Wed, 10 Aug 2022 05:01:41 GMT

←Older revision		Revision as of 05:01, 10 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vvz ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7vvz' size='340' side='right'caption='[[7vvz]]' scene=''>
-	~~Authors~~: ~~Qu, K~~.~~, Chen, Z~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: NuA4 bound to~~ the ~~nucleosome~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vvz OCA], [https://pdbe.org/7vvz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vvz RCSB], [https://www.ebi.ac.uk/pdbsum/7vvz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vvz ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Qu, K~~]]	+	__TOC__
-	[[Category: ~~Chen,~~ Z]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 14:53, 6 July 2022

OCA — Wed, 06 Jul 2022 14:53:10 GMT

←Older revision		Revision as of 14:53, 6 July 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vvz is ON HOLD ~~until Paper Publication~~	+	The entry 7vvz is ON HOLD

	Authors: Qu, K., Chen, Z.		Authors: Qu, K., Chen, Z.

OCA at 06:42, 25 May 2022

OCA — Wed, 25 May 2022 06:42:26 GMT

←Older revision		Revision as of 06:42, 25 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vvz is ON HOLD	+	The entry 7vvz is ON HOLD until Paper Publication

	Authors: Qu, K., Chen, Z.		Authors: Qu, K., Chen, Z.

OCA: Protected "7vvz" [edit=sysop:move=sysop]

OCA — Wed, 17 Nov 2021 12:20:56 GMT

Protected "7vvz" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:20, 17 November 2021

OCA: New page: '''Unreleased structure''' The entry 7vvz is ON HOLD Authors: Qu, K., Chen, Z. Description: NuA4 bound to the nucleosome Category: Unreleased Structures Category: Qu, K [[Categ...

OCA — Wed, 17 Nov 2021 12:20:55 GMT

New page: '''Unreleased structure''' The entry 7vvz is ON HOLD Authors: Qu, K., Chen, Z. Description: NuA4 bound to the nucleosome Category: Unreleased Structures Category: Qu, K [[Categ...

New page

'''Unreleased structure'''

The entry 7vvz is ON HOLD

Authors: Qu, K., Chen, Z.

Description: NuA4 bound to the nucleosome
[[Category: Unreleased Structures]]
[[Category: Qu, K]]
[[Category: Chen, Z]]