7vxq - Revision history

OCA at 17:33, 29 November 2023

OCA — Wed, 29 Nov 2023 17:33:49 GMT

←Older revision		Revision as of 17:33, 29 November 2023
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-	====	+	==The Carbon Monoxide Complex of [NiFe]-hydrogenase (Hyb-type) from Citrobacter sp. S-77==
-	<StructureSection load='7vxq' size='340' side='right'caption='[[7vxq]]' scene=''>	+	<StructureSection load='7vxq' size='340' side='right'caption='[[7vxq]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vxq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_sp._S-77 Citrobacter sp. S-77]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VXQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vxq OCA], [https://pdbe.org/7vxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vxq RCSB], [https://www.ebi.ac.uk/pdbsum/7vxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vxq ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.77Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFU:FORMYL[BIS(HYDROCYANATO-1KAPPAC)]IRONNICKEL(FE-NI)'>NFU</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vxq OCA], [https://pdbe.org/7vxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vxq RCSB], [https://www.ebi.ac.uk/pdbsum/7vxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vxq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A3B6UEQ1_9ENTR A0A3B6UEQ1_9ENTR]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hydrogenases catalyze the reversible oxidation of H(2). Carbon monoxide (CO) is known to be a competitive inhibitor of O(2)-sensitive [NiFe]-hydrogenases. Although the activities of some O(2)-tolerant [NiFe]-hydrogenases are unaffected by CO, the partially O(2)-tolerant [NiFe]-hydrogenase from Citrobacter sp. S-77 (S77-HYB) is inhibited by CO. In this work, the CO-bound state of S77-HYB was characterized by activity assays, spectroscopic techniques and X-ray crystallography. Electron paramagnetic resonance spectroscopy showed a diamagnetic Ni(2+) state, and Fourier-transform infrared spectroscopy revealed the stretching vibration of the exogenous CO ligand. The crystal structure determined at 1.77 A resolution revealed that CO binds weakly to the nickel ion in the Ni-Fe active site of S77-HYB. These results suggest a positive correlation between O(2) and CO tolerance in [NiFe]-hydrogenases.
		+
		+	Structural and spectroscopic characterization of CO inhibition of [NiFe]-hydrogenase from Citrobacter sp. S-77.,Imanishi T, Nishikawa K, Taketa M, Higuchi K, Tai H, Hirota S, Hojo H, Kawakami T, Hataguchi K, Matsumoto K, Ogata H, Higuchi Y Acta Crystallogr F Struct Biol Commun. 2022 Feb 1;78(Pt 2):66-74. doi: , 10.1107/S2053230X22000188. Epub 2022 Jan 27. PMID:35102895<ref>PMID:35102895</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vxq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Citrobacter sp. S-77]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Higuchi K]]
		+	[[Category: Higuchi Y]]
		+	[[Category: Imanishi T]]
		+	[[Category: Nishikawa K]]

OCA at 07:27, 9 February 2022

OCA — Wed, 09 Feb 2022 07:27:17 GMT

←Older revision		Revision as of 07:27, 9 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vxq ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7vxq' size='340' side='right'caption='[[7vxq]]' scene=''>
-	~~Authors~~: ~~Nishikawa, K~~.~~, Higuchi, K~~.~~, Imanishi, T~~.~~, Higuchi, Y~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~The Carbon Monoxide Complex of [NiFe~~]~~-hydrogenase (Hyb-type) from Citrobacter sp~~. ~~S-77~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vxq OCA], [https://pdbe.org/7vxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vxq RCSB], [https://www.ebi.ac.uk/pdbsum/7vxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vxq ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Nishikawa~~, K]]	+	__TOC__
-	[~~[Category~~: ~~Higuchi~~, Y]]	+	</StructureSection>
-	[[Category: ~~Imanishi, T~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Higuchi, K~~]]	+	[[Category: Z-disk]]

OCA at 12:31, 29 December 2021

OCA — Wed, 29 Dec 2021 12:31:39 GMT

←Older revision		Revision as of 12:31, 29 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vxq is ON HOLD	+	The entry 7vxq is ON HOLD until Paper Publication

	Authors: Nishikawa, K., Higuchi, K., Imanishi, T., Higuchi, Y.		Authors: Nishikawa, K., Higuchi, K., Imanishi, T., Higuchi, Y.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Nishikawa, K]]		[[Category: Nishikawa, K]]
-	[[Category: Imanishi, T]]
	[[Category: Higuchi, Y]]		[[Category: Higuchi, Y]]
		+	[[Category: Imanishi, T]]
	[[Category: Higuchi, K]]		[[Category: Higuchi, K]]

OCA: Protected "7vxq" [edit=sysop:move=sysop]

OCA — Wed, 24 Nov 2021 11:05:13 GMT

Protected "7vxq" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:05, 24 November 2021

OCA: New page: '''Unreleased structure''' The entry 7vxq is ON HOLD Authors: Nishikawa, K., Higuchi, K., Imanishi, T., Higuchi, Y. Description: The Carbon Monoxide Complex of [NiFe]-hydrogenase (Hyb-...

OCA — Wed, 24 Nov 2021 11:05:12 GMT

New page: '''Unreleased structure''' The entry 7vxq is ON HOLD Authors: Nishikawa, K., Higuchi, K., Imanishi, T., Higuchi, Y. Description: The Carbon Monoxide Complex of [NiFe]-hydrogenase (Hyb-...

New page

'''Unreleased structure'''

The entry 7vxq is ON HOLD

Authors: Nishikawa, K., Higuchi, K., Imanishi, T., Higuchi, Y.

Description: The Carbon Monoxide Complex of [NiFe]-hydrogenase (Hyb-type) from Citrobacter sp. S-77
[[Category: Unreleased Structures]]
[[Category: Nishikawa, K]]
[[Category: Imanishi, T]]
[[Category: Higuchi, Y]]
[[Category: Higuchi, K]]