OCA at 10:02, 14 December 2022

OCA — Wed, 14 Dec 2022 10:02:31 GMT

←Older revision		Revision as of 10:02, 14 December 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7vy9 is ~~ON HOLD~~	+	==Membrane arm of active state CI from Q10-NADH dataset==
		+	<StructureSection load='7vy9' size='340' side='right'caption='[[7vy9]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7vy9]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VY9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VY9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8Q1:S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]+dodecanethioate'>8Q1</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene>, <scene name='pdbligand=PEE:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>PEE</scene>, <scene name='pdbligand=PLX:(9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL'>PLX</scene>, <scene name='pdbligand=UQ:COENZYME+Q10,+(2Z,6E,10Z,14E,18E,22E,26Z)-ISOMER'>UQ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vy9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vy9 OCA], [https://pdbe.org/7vy9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vy9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vy9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vy9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A480JYS1_PIG A0A480JYS1_PIG]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mammalian respiratory complex I (CI) is a 45-subunit, redox-driven proton pump that generates an electrochemical gradient across the mitochondrial inner membrane to power ATP synthesis in mitochondria. In the present study, we report cryo-electron microscopy structures of CI from Sus scrofa in six treatment conditions at a resolution of 2.4-3.5 A, in which CI structures of each condition can be classified into two biochemical classes (active or deactive), with a notably higher proportion of active CI particles. These structures illuminate how hydrophobic ubiquinone-10 (Q10) with its long isoprenoid tail is bound and reduced in a narrow Q chamber comprising four different Q10-binding sites. Structural comparisons of active CI structures from our decylubiquinone-NADH and rotenone-NADH datasets reveal that Q10 reduction at site 1 is not coupled to proton pumping in the membrane arm, which might instead be coupled to Q10 oxidation at site 2. Our data overturn the widely accepted previous proposal about the coupling mechanism of CI.

-	~~Authors: Gu~~, J~~.K.~~, ~~Yang~~, M.J.	+	The coupling mechanism of mammalian mitochondrial complex I.,Gu J, Liu T, Guo R, Zhang L, Yang M Nat Struct Mol Biol. 2022 Feb;29(2):172-182. doi: 10.1038/s41594-022-00722-w. , Epub 2022 Feb 10. PMID:35145322<ref>PMID:35145322</ref>

-	~~Description: Membrane arm~~ of ~~active state CI from Q10~~-~~NADH dataset~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Gu~~, J.K~~]]	+	<div class="pdbe-citations 7vy9" style="background-color:#fffaf0;"></div>
-	[[Category: Yang~~, M.J~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Sus scrofa]]
		+	[[Category: Gu JK]]
		+	[[Category: Yang MJ]]

OCA: Protected "7vy9" [edit=sysop:move=sysop]

OCA — Wed, 24 Nov 2021 11:06:15 GMT

Protected "7vy9" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:06, 24 November 2021

OCA: New page: '''Unreleased structure''' The entry 7vy9 is ON HOLD Authors: Gu, J.K., Yang, M.J. Description: Membrane arm of active state CI from Q10-NADH dataset [[Category: Unreleased Structures]...

OCA — Wed, 24 Nov 2021 11:06:14 GMT

New page: '''Unreleased structure''' The entry 7vy9 is ON HOLD Authors: Gu, J.K., Yang, M.J. Description: Membrane arm of active state CI from Q10-NADH dataset [[Category: Unreleased Structures]...

New page

'''Unreleased structure'''

The entry 7vy9 is ON HOLD

Authors: Gu, J.K., Yang, M.J.

Description: Membrane arm of active state CI from Q10-NADH dataset
[[Category: Unreleased Structures]]
[[Category: Gu, J.K]]
[[Category: Yang, M.J]]

7vy9 - Revision history

OCA at 10:02, 14 December 2022

OCA: Protected "7vy9" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7vy9 is ON HOLD Authors: Gu, J.K., Yang, M.J. Description: Membrane arm of active state CI from Q10-NADH dataset [[Category: Unreleased Structures]...